Salmonella Typhoid Toxin PltB Subunit and Its Non-typhoidal Salmonella Ortholog Confer Differential Host Adaptation and Virulence

Lee, Sohyoung; Yang, Yi-An; Milano, Shawn K.; Nguyen, Tri; Ahn, Changhwan; Sim, Ji Hyun; Thompson, Andrew J.; Hillpot, Eric C.; Yoo, Gyeongshik; Paulson, James C.; Song, Jeongmin

doi:10.1016/j.chom.2020.04.005

Salmonella Typhoid Toxin PltB Subunit and Its Non-typhoidal Salmonella Ortholog Confer Differential Host Adaptation and Virulence

Journal Article · Mon May 11 00:00:00 EDT 2020 · Cell Host & Microbe

DOI:https://doi.org/10.1016/j.chom.2020.04.005· OSTI ID:1834871

Lee, Sohyoung ^[1]; Yang, Yi-An ^[1]; Milano, Shawn K. ^[2]; Nguyen, Tri ^[1]; Ahn, Changhwan ^[1]; Sim, Ji Hyun ^[1]; Thompson, Andrew J. ^[3]; Hillpot, Eric C. ^[2]; Yoo, Gyeongshik ^[1]; Paulson, James C. ^[3]; Song, Jeongmin ^[1]

Cornell Univ. College of Veterinary Medicine, Ithaca, NY (United States). Dept. of Microbiology and Immunology
Cornell Univ. College of Veterinary Medicine, Ithaca, NY (United States). Dept. of Molecular Medicine
The Scripps Research Inst., La Jolla, CA (United States). Dept. of Molecular Medicine

Typhoidal and non-typhoidal Salmonelleae (NTS) cause typhoid fever and gastroenteritis, respectively, in humans. Salmonella typhoid toxin contributes to typhoid disease progression and chronic infection, but little is known about the role of its NTS ortholog. We found that typhoid toxin and its NTS ortholog induce different clinical presentations. The PltB subunit of each toxin exhibits different glycan-binding preferences that correlate with glycan expression profiles of host cells targeted by each bacterium at the primary infection or intoxication sites. Through co-crystal structures of PltB subunits bound to specific glycan receptor moieties, we show that they induce markedly different glycan-binding preferences and virulence outcomes. Furthermore, immunization with the NTS S. Javiana or its toxin offers cross-reactive protection against lethal-dose typhoid toxin challenge. Cumulatively, these results offer insights into the evolution of host adaptations in Salmonella AB toxins, their cell and tissue tropisms, and the design for improved typhoid vaccines and therapeutics.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC)

Grant/Contract Number:: AC02-06CH11357

OSTI ID:: 1834871

Alternate ID(s):: OSTI ID: 1839763
OSTI ID: 1632976

Journal Information:: Cell Host & Microbe, Journal Name: Cell Host & Microbe Journal Issue: 6 Vol. 27; ISSN 1931-3128

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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59 BASIC BIOLOGICAL SCIENCES
Salmonella
bacterial AB toxins
cross-reactive protection or immunity
glycan expression
glycans
host adaptations
receptor-binding
structure of protein toxins
typhoid fever
typhoid toxin

Salmonella Typhoid Toxin PltB Subunit and Its Non-typhoidal Salmonella Ortholog Confer Differential Host Adaptation and Virulence

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