Mechanisms of substrate recognition by a typhoid toxin secretion-associated muramidase

Geiger, Tobias; Lara-Tejero, Maria; Xiong, Yong; Galán, Jorge E.

doi:10.7554/eLife.53473

Mechanisms of substrate recognition by a typhoid toxin secretion-associated muramidase

Journal Article · Mon Jan 20 00:00:00 EST 2020 · eLife

DOI:https://doi.org/10.7554/eLife.53473· OSTI ID:1600820

^[1]; ^[1]; Xiong, Yong ^[1]; ^[1]

Yale Univ. School of Medicine, New Haven, CT (United States)

Typhoid toxin is a virulence factor for the bacterial pathogen Salmonella Typhi, which causes typhoid fever in humans. After its synthesis by intracellular bacteria, typhoid toxin is secreted into the lumen of the Salmonella-containing vacuole by a secretion mechanism strictly dependent on TtsA, a specific muramidase that facilitates toxin transport through the peptidoglycan layer. Here we show that substrate recognition by TtsA depends on a discrete domain within its carboxy terminus, which targets the enzyme to the bacterial poles to recognize YcbB-edited peptidoglycan. Comparison of the atomic structures of TtsA bound to its substrate and that of a close homolog with different specificity identified specific determinants involved in substrate recognition. Combined with structure-guided mutagenesis and in vitro and in vivo crosslinking experiments, this study provides an unprecedented view of the mechanisms by which a muramidase recognizes its peptidoglycan substrate to facilitate protein secretion.

View Accepted Manuscript (DOE)

Research Organization:: Argonne National Laboratory (ANL), Argonne, IL (United States)

Sponsoring Organization:: National Inst. of Allergy and Infectious Diseases; National Inst. of General Medical Sciences (NIGMS); USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)

Grant/Contract Number:: AC02-06CH11357; SC0012704

OSTI ID:: 1600820

Alternate ID(s):: OSTI ID: 1760481

Journal Information:: eLife, Journal Name: eLife Journal Issue: 01, 2020 Vol. 9; ISSN 2050-084X

Publisher:: eLife Sciences Publications, Ltd.Copyright Statement

Country of Publication:: United States

Language:: ENGLISH

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