D1-S169A substitution of photosystem II reveals a novel S2-state structure

Ghosh, Ipsita; Banerjee, Gourab; Reiss, Krystle; Kim, Christopher J.; Debus, Richard J.; Batista, Victor S.; Brudvig, Gary W.

doi:10.1016/j.bbabio.2020.148301

Title: D1-S169A substitution of photosystem II reveals a novel S2-state structure

Journal Article · Tue Dec 01 00:00:00 EST 2020 · Biochimica et Biophysica Acta - Bioenergetics

DOI:https://doi.org/10.1016/j.bbabio.2020.148301· OSTI ID:1833470

Ghosh, Ipsita; Banerjee, Gourab; Reiss, Krystle; Kim, Christopher J.; Debus, Richard J.; Batista, Victor S.; Brudvig, Gary W.

In photosystem II (PSII), photosynthetic water oxidation occurs at the O₂-evolving complex (OEC), a tetramanganese-calcium cluster that cycles through light-induced redox intermediates (S₀ - S₄) to produce oxygen from two substrate water molecules. The OEC is surrounded by a hydrogen-bonded network of amino-acid residues that plays a crucial role in proton transfer and substrate water delivery. Previously, we found that D1-S169 was crucial for water oxidation and its mutation to alanine perturbed the hydrogen-bonding network. In this study, we demonstrate that the activation energy for the S₂ to S₁ transition of D1-S169A PSII is higher than wild-type PSII with a ~1.7 - 2.7x slower rate of charge recombination with Q_A¯ relative to wild-type PSII. Arrhenius analysis of the decay kinetics shows an Ea of 5.87 ± 1.15 kcal mol^–1 for decay back to the S1 state, compared to 0.80 ± 0.13 kcal mol^–1 for the wild-type S₂ state. In addition, we find that ammonia does not affect the S₂-state EPR signal, indicating that ammonia does not bind to the Mn cluster in D1-S169A PSII. Lastly, a QM/MM analysis indicates that an additional water molecule binds to the Mn4 ion in place of an oxo ligand O5 in the S₂ state of D1-S169A PSII. The altered S₂ state of D1-S169A PSII provides insight into the S₂ → S₃ state transition.

View Journal Article

Cite

Export

Save

Research Organization:: Yale Univ., New Haven, CT (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: FG02-05ER15646; SC0005291; SC0001423

OSTI ID:: 1833470

Alternate ID(s):: OSTI ID: 1658414; OSTI ID: 1812293

Journal Information:: Biochimica et Biophysica Acta - Bioenergetics, Journal Name: Biochimica et Biophysica Acta - Bioenergetics Vol. 1861 Journal Issue: 12; ISSN 0005-2728

Publisher:: ElsevierCopyright Statement

Country of Publication:: Netherlands

Language:: English

References (69)

S ₀ -State Model of the Oxygen-Evolving Complex of Photosystem II Pal, Rhitankar; Negre, Christian F. A.; Vogt, Leslie Biochemistry, Vol. 52, Issue 44 https://doi.org/10.1021/bi401214v	journal	October 2013
Structure, ligands and substrate coordination of the oxygen-evolving complex of photosystem II in the S2 state: a combined EPR and DFT study Lohmiller, Thomas; Krewald, Vera; Navarro, Montserrat Pérez Physical Chemistry Chemical Physics, Vol. 16, Issue 24 https://doi.org/10.1039/c3cp55017f	journal	January 2014
Evidence from Biosynthetically Incorporated Strontium and FTIR Difference Spectroscopy that the C-Terminus of the D1 Polypeptide of Photosystem II Does Not Ligate Calcium ^† Strickler, Melodie A.; Walker, Lee M.; Hillier, Warwick Biochemistry, Vol. 44, Issue 24 https://doi.org/10.1021/bi050653y	journal	June 2005
Thermodynamics of the S ₂ -to-S ₃ state transition of the oxygen-evolving complex of photosystem II Amin, Muhamed; Kaur, Divya; Yang, Ke R. Physical Chemistry Chemical Physics, Vol. 21, Issue 37 https://doi.org/10.1039/C9CP02308A	journal	January 2019
Mechanism of tyrosine D oxidation in Photosystem II Saito, K.; Rutherford, A. W.; Ishikita, H. Proceedings of the National Academy of Sciences, Vol. 110, Issue 19 https://doi.org/10.1073/pnas.1300817110	journal	April 2013
Ab initio effective core potentials for molecular calculations. Potentials for K to Au including the outermost core orbitals Hay, P. Jeffrey; Wadt, Willard R. The Journal of Chemical Physics, Vol. 82, Issue 1, p. 299-310 https://doi.org/10.1063/1.448975	journal	January 1985
Oxygen-evolving complex of photosystem II: correlating structure with spectroscopy Pokhrel, Ravi; Brudvig, Gary W. Physical Chemistry Chemical Physics, Vol. 16, Issue 24 https://doi.org/10.1039/c4cp00493k	journal	January 2014
Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å Umena, Yasufumi; Kawakami, Keisuke; Shen, Jian-Ren Nature, Vol. 473, Issue 7345 https://doi.org/10.1038/nature09913	journal	April 2011
EPR signals from modified charge accumulation states of the oxygen-evolving enzyme in calcium-deficient photosystem II Boussac, Alain; Zimmermann, Jean Luc; Rutherford, A. William Biochemistry, Vol. 28, Issue 23 https://doi.org/10.1021/bi00449a005	journal	November 1989
Photosystem II and the unique role of bicarbonate: A historical perspective Shevela, Dmitriy; Eaton-Rye, Julian J.; Shen, Jian-Ren Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 8 https://doi.org/10.1016/j.bbabio.2012.04.003	journal	August 2012
Ammonia binds to the manganese site of the oxygen-evolving complex of photosystem II in the S2 state Beck, Warren F.; De Paula, Julio C.; Brudvig, Gary W. Journal of the American Chemical Society, Vol. 108, Issue 14 https://doi.org/10.1021/ja00274a027	journal	July 1986
Glutamate 189 of the D1 Polypeptide Modulates the Magnetic and Redox Properties of the Manganese Cluster and Tyrosine Y _Z in Photosystem II ^† Debus, Richard J.; Campbell, Kristy A.; Pham, Donna P. Biochemistry, Vol. 39, Issue 21 https://doi.org/10.1021/bi992749w	journal	May 2000
18O-Water exchange in photosystem II: Substrate binding and intermediates of the water splitting cycle Hillier, W.; Wydrzynski, T. Coordination Chemistry Reviews, Vol. 252, Issue 3-4 https://doi.org/10.1016/j.ccr.2007.09.004	journal	February 2008
Functional Roles of D2-Lys317 and the Interacting Chloride Ion in the Water Oxidation Reaction of Photosystem II As Revealed by Fourier Transform Infrared Analysis Suzuki, Hiroyuki; Yu, Jianfeng; Kobayashi, Takashi Biochemistry, Vol. 52, Issue 28 https://doi.org/10.1021/bi301699h	journal	July 2013
Missing Pieces in the Puzzle of Biological Water Oxidation Pantazis, Dimitrios A. ACS Catalysis, Vol. 8, Issue 10 https://doi.org/10.1021/acscatal.8b01928	journal	September 2018
Insights into Substrate Binding to the Oxygen-Evolving Complex of Photosystem II from Ammonia Inhibition Studies Vinyard, David J.; Brudvig, Gary W. Biochemistry, Vol. 54, Issue 2 https://doi.org/10.1021/bi5014134	journal	December 2014
Probing the role of Valine 185 of the D1 protein in the Photosystem II oxygen evolution Sugiura, Miwa; Tibiletti, Tania; Takachi, Itsuki Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1859, Issue 12 https://doi.org/10.1016/j.bbabio.2018.10.003	journal	December 2018
Directed alteration of the D1 polypeptide of photosystem II: evidence that tyrosine-161 is the redox component, Z, connecting the oxygen-evolving complex to the primary electron donor, P680 Metz, James G.; Nixon, Peter J.; Rogner, Matthias Biochemistry, Vol. 28, Issue 17 https://doi.org/10.1021/bi00443a028	journal	August 1989
Eight steps preceding O–O bond formation in oxygenic photosynthesis—A basic reaction cycle of the Photosystem II manganese complex Dau, Holger; Haumann, Michael Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1767, Issue 6 https://doi.org/10.1016/j.bbabio.2007.02.022	journal	June 2007
Perturbing the Water Cavity Surrounding the Manganese Cluster by Mutating the Residue D1-Valine 185 Has a Strong Effect on the Water Oxidation Mechanism of Photosystem II Dilbeck, Preston L.; Bao, Han; Neveu, Curtis L. Biochemistry, Vol. 52, Issue 39 https://doi.org/10.1021/bi400930g	journal	September 2013
Oxygen-evolving complex of Photosystem II: an analysis of second-shell residues and hydrogen-bonding networks Vogt, Leslie; Vinyard, David J.; Khan, Sahr Current Opinion in Chemical Biology, Vol. 25 https://doi.org/10.1016/j.cbpa.2014.12.040	journal	April 2015
Light-induced quinone reduction in photosystem II Müh, Frank; Glöckner, Carina; Hellmich, Julia Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1817, Issue 1 https://doi.org/10.1016/j.bbabio.2011.05.021	journal	January 2012
Interaction of ammonia with the water splitting enzyme of photosystem II Boussac, Alain; Rutherford, A. William; Styring, Stenbjorn Biochemistry, Vol. 29, Issue 1 https://doi.org/10.1021/bi00453a003	journal	January 1990
Ammonia Binds to the Dangler Manganese of the Photosystem II Oxygen-Evolving Complex Oyala, Paul H.; Stich, Troy A.; Debus, Richard J. Journal of the American Chemical Society, Vol. 137, Issue 27 https://doi.org/10.1021/jacs.5b04768	journal	July 2015
Effect of Ca ²⁺ /Sr ²⁺ Substitution on the Electronic Structure of the Oxygen-Evolving Complex of Photosystem II: A Combined Multifrequency EPR, ⁵⁵ Mn-ENDOR, and DFT Study of the S ₂ State Cox, Nicholas; Rapatskiy, Leonid; Su, Ji-Hu Journal of the American Chemical Society, Vol. 133, Issue 10 https://doi.org/10.1021/ja110145v	journal	March 2011
One of the Substrate Waters for O ₂ Formation in Photosystem II Is Provided by the Water-Splitting Mn ₄ CaO ₅ Cluster’s Ca ²⁺ Ion Kim, Christopher J.; Debus, Richard J. Biochemistry, Vol. 58, Issue 29 https://doi.org/10.1021/acs.biochem.9b00418	journal	June 2019
Density‐functional thermochemistry. III. The role of exact exchange Becke, Axel D. The Journal of Chemical Physics, Vol. 98, Issue 7, p. 5648-5652 https://doi.org/10.1063/1.464913	journal	April 1993
Structural Effects of Ammonia Binding to the Mn ₄ CaO ₅ Cluster of Photosystem II Marchiori, David A.; Oyala, Paul H.; Debus, Richard J. The Journal of Physical Chemistry B, Vol. 122, Issue 5 https://doi.org/10.1021/acs.jpcb.7b11101	journal	January 2018
Inhibitory and Non-Inhibitory NH ₃ Binding at the Water-Oxidizing Manganese Complex of Photosystem II Suggests Possible Sites and a Rearrangement Mode of Substrate Water Molecules Schuth, Nils; Liang, Zhiyong; Schönborn, Matthias Biochemistry, Vol. 56, Issue 47 https://doi.org/10.1021/acs.biochem.7b00743	journal	November 2017
Site-Directed Photosystem II Mutants with Perturbed Oxygen-Evolving Properties. 1. Instability or Inefficient Assembly of the Manganese Cluster In vivo Chu, Hsiu-An; Nguyen, Anh P.; Debus, Richard J. Biochemistry, Vol. 33, Issue 20 https://doi.org/10.1021/bi00186a013	journal	May 1994
Mechanism of Methanol Inhibition of Photosynthetic Water Oxidation As Studied by Fourier Transform Infrared Difference and Time-Resolved Infrared Spectroscopies Yata, Haruna; Noguchi, Takumi Biochemistry, Vol. 57, Issue 32 https://doi.org/10.1021/acs.biochem.8b00596	journal	July 2018
The influence of polarization functions on molecular orbital hydrogenation energies Hariharan, P. C.; Pople, J. A. Theoretica Chimica Acta, Vol. 28, Issue 3 https://doi.org/10.1007/BF00533485	journal	January 1973
Impact of D1-V185 on the Water Molecules That Facilitate O ₂ Formation by the Catalytic Mn ₄ CaO ₅ Cluster in Photosystem II Kim, Christopher J.; Bao, Han; Burnap, Robert L. Biochemistry, Vol. 57, Issue 29 https://doi.org/10.1021/acs.biochem.8b00630	journal	June 2018
Progress Toward a Molecular Mechanism of Water Oxidation in Photosystem II Vinyard, David J.; Brudvig, Gary W. Annual Review of Physical Chemistry, Vol. 68, Issue 1 https://doi.org/10.1146/annurev-physchem-052516-044820	journal	May 2017
Native structure of photosystem II at 1.95 Å resolution viewed by femtosecond X-ray pulses Suga, Michihiro; Akita, Fusamichi; Hirata, Kunio Nature, Vol. 517, Issue 7532 https://doi.org/10.1038/nature13991	journal	November 2014
The ONIOM (our own N-layered integrated molecular orbital + molecular mechanics) method for the first singlet excited (S1) state photoisomerization path of a retinal protonated Schiff base Vreven, Thom; Morokuma, Keiji The Journal of Chemical Physics, Vol. 113, Issue 8 https://doi.org/10.1063/1.1287059	journal	August 2000
Bicarbonate rescues damaged proton-transfer pathway in photosystem II Banerjee, Gourab; Ghosh, Ipsita; Kim, Christopher J. Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1860, Issue 8 https://doi.org/10.1016/j.bbabio.2019.06.014	journal	August 2019
Pulse Electron Paramagnetic Resonance Studies of the Interaction of Methanol with the S ₂ State of the Mn ₄ O ₅ Ca Cluster of Photosystem II Oyala, Paul H.; Stich, Troy A.; Stull, Jamie A. Biochemistry, Vol. 53, Issue 50 https://doi.org/10.1021/bi501323h	journal	December 2014
Hole-vibronic coupling in oligothiophenes: impact of backbone torsional flexibility on relaxation energies da Silva Filho, Demetrio A.; Coropceanu, Veaceslav; Fichou, Denis Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences, Vol. 365, Issue 1855 https://doi.org/10.1098/rsta.2007.2025	journal	April 2007
Light-induced structural changes and the site of O=O bond formation in PSII caught by XFEL Suga, Michihiro; Akita, Fusamichi; Sugahara, Michihiro Nature, Vol. 543, Issue 7643 https://doi.org/10.1038/nature21400	journal	February 2017
An oxyl/oxo mechanism for oxygen-oxygen coupling in PSII revealed by an x-ray free-electron laser Suga, Michihiro; Akita, Fusamichi; Yamashita, Keitaro Science, Vol. 366, Issue 6463 https://doi.org/10.1126/science.aax6998	journal	October 2019
Relative stability of the S2 isomers of the oxygen evolving complex of photosystem II Kaur, Divya; Szejgis, Witold; Mao, Junjun Photosynthesis Research, Vol. 141, Issue 3 https://doi.org/10.1007/s11120-019-00637-6	journal	April 2019
Interaction of methanol with the oxygen-evolving complex: atomistic models, channel identification, species dependence, and mechanistic implications Retegan, Marius; Pantazis, Dimitrios A. Chemical Science, Vol. 7, Issue 10 https://doi.org/10.1039/C6SC02340A	journal	January 2016
Two Interconvertible Structures that Explain the Spectroscopic Properties of the Oxygen-Evolving Complex of Photosystem II in the S ₂ State Pantazis, Dimitrios A.; Ames, William; Cox, Nicholas Angewandte Chemie International Edition, Vol. 51, Issue 39 https://doi.org/10.1002/anie.201204705	journal	August 2012
Thermodynamically accurate modeling of the catalytic cycle of photosynthetic oxygen evolution: A mathematical solution to asymmetric Markov chains Vinyard, David J.; Zachary, Chase E.; Ananyev, Gennady Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 1827, Issue 7 https://doi.org/10.1016/j.bbabio.2013.04.008	journal	July 2013
The O ₂ -Evolving Complex of Photosystem II: Recent Insights from Quantum Mechanics/Molecular Mechanics (QM/MM), Extended X-ray Absorption Fine Structure (EXAFS), and Femtosecond X-ray Crystallography Data Askerka, Mikhail; Brudvig, Gary W.; Batista, Victor S. Accounts of Chemical Research, Vol. 50, Issue 1 https://doi.org/10.1021/acs.accounts.6b00405	journal	December 2016
The Basic Properties of the Electronic Structure of the Oxygen-evolving Complex of Photosystem II Are Not Perturbed by Ca ²⁺ Removal Lohmiller, Thomas; Cox, Nicholas; Su, Ji-Hu Journal of Biological Chemistry, Vol. 287, Issue 29 https://doi.org/10.1074/jbc.M112.365288	journal	May 2012
Deactivation kinetics and temperature dependence of the S-state transitions in the oxygen-evolving system of Photosystem II measured by EPR spectroscopy Styring, Stenbjörn; Rutherford, A. William Biochimica et Biophysica Acta (BBA) - Bioenergetics, Vol. 933, Issue 2 https://doi.org/10.1016/0005-2728(88)90046-1	journal	April 1988
D1-Asp170 Is Structurally Coupled to the Oxygen Evolving Complex in Photosystem II As Revealed by Light-Induced Fourier Transform Infrared Difference Spectroscopy ^† Chu, Hsiu-An; Debus, Richard J.; Babcock, Gerald T. Biochemistry, Vol. 40, Issue 7 https://doi.org/10.1021/bi0022994	journal	February 2001
Biochemical and Spectroscopic Characterization of a New Oxygen-Evolving Photosystem II Core Complex from the Cyanobacterium Synechocystis PCC 6803 Tang, Xiao-Song; Diner, Bruce A. Biochemistry, Vol. 33, Issue 15 https://doi.org/10.1021/bi00181a021	journal	April 1994
Structural Changes in the Oxygen-Evolving Complex of Photosystem II Induced by the S ₁ to S ₂ Transition: A Combined XRD and QM/MM Study Askerka, Mikhail; Wang, Jimin; Brudvig, Gary W. Biochemistry, Vol. 53, Issue 44 https://doi.org/10.1021/bi5011915	journal	October 2014
Stability of the S ₃ and S ₂ State Intermediates in Photosystem II Directly Probed by EPR Spectroscopy Chen, Guiying; Han, Guangye; Göransson, Erik Biochemistry, Vol. 51, Issue 1 https://doi.org/10.1021/bi200627j	journal	December 2011
Location of Methanol on the S ₂ State Mn Cluster in Photosystem II Studied by Proton Matrix Electron Nuclear Double Resonance Nagashima, Hiroki; Mino, Hiroyuki The Journal of Physical Chemistry Letters, Vol. 8, Issue 3 https://doi.org/10.1021/acs.jpclett.7b00110	journal	January 2017
Electronic structure of the oxygen-evolving complex in photosystem II prior to O-O bond formation Cox, N.; Retegan, M.; Neese, F. Science, Vol. 345, Issue 6198 https://doi.org/10.1126/science.1254910	journal	August 2014
COOPERATION OF CHARGES IN PHOTOSYNTHETIC O ₂ EVOLUTION–I. A LINEAR FOUR STEP MECHANISM Kok, Bessel; Forbush, Bliss; McGLOIN, Marion Photochemistry and Photobiology, Vol. 11, Issue 6 https://doi.org/10.1111/j.1751-1097.1970.tb06017.x	journal	June 1970
Recent developments in biological water oxidation Pérez-Navarro, Montserrat; Neese, Frank; Lubitz, Wolfgang Current Opinion in Chemical Biology, Vol. 31 https://doi.org/10.1016/j.cbpa.2016.02.007	journal	April 2016
Ammonia binding to the oxygen-evolving complex of photosystem II identifies the solvent-exchangeable oxygen bridge ( -oxo) of the manganese tetramer Perez Navarro, M.; Ames, W. M.; Nilsson, H. Proceedings of the National Academy of Sciences, Vol. 110, Issue 39 https://doi.org/10.1073/pnas.1304334110	journal	September 2013
Substrate–water exchange in photosystem II is arrested before dioxygen formation Nilsson, Håkan; Rappaport, Fabrice; Boussac, Alain Nature Communications, Vol. 5, Issue 1 https://doi.org/10.1038/ncomms5305	journal	July 2014
D1-S169A Substitution of Photosystem II Perturbs Water Oxidation Ghosh, Ipsita; Banerjee, Gourab; Kim, Christopher J. Biochemistry, Vol. 58, Issue 10 https://doi.org/10.1021/acs.biochem.8b01184	journal	February 2019
The microwave power saturation of SIIslow varies with the redox state of the oxygen-evolving complex in photosystem II Styring, Stenbjoern A.; Rutherford, A. William Biochemistry, Vol. 27, Issue 13 https://doi.org/10.1021/bi00413a049	journal	June 1988
Binding of amines to the oxygen-evolving center of photosystem II Beck, Warren F.; Brudvig, Gary W. Biochemistry, Vol. 25, Issue 21 https://doi.org/10.1021/bi00369a021	journal	October 1986
Insights into Proton-Transfer Pathways during Water Oxidation in Photosystem II Ghosh, Ipsita; Khan, Sahr; Banerjee, Gourab The Journal of Physical Chemistry B, Vol. 123, Issue 39 https://doi.org/10.1021/acs.jpcb.9b06244	journal	September 2019
Substitution of the D1-Asn87 site in photosystem II of cyanobacteria mimics the chloride-binding characteristics of spinach photosystem II Banerjee, Gourab; Ghosh, Ipsita; Kim, Christopher J. Journal of Biological Chemistry, Vol. 293, Issue 7 https://doi.org/10.1074/jbc.M117.813170	journal	February 2018
Role of the O4 Channel in Photosynthetic Water Oxidation as Revealed by Fourier Transform Infrared Difference and Time-Resolved Infrared Analysis of the D1-S169A Mutant Shimada, Yuichiro; Kitajima-Ihara, Tomomi; Nagao, Ryo The Journal of Physical Chemistry B, Vol. 124, Issue 8 https://doi.org/10.1021/acs.jpcb.9b11946	journal	February 2020
Structures of the intermediates of Kok’s photosynthetic water oxidation clock Kern, Jan; Chatterjee, Ruchira; Young, Iris D. Nature, Vol. 563, Issue 7731 https://doi.org/10.1038/s41586-018-0681-2	journal	November 2018
Molecular Catalysts for Water Oxidation Blakemore, James D.; Crabtree, Robert H.; Brudvig, Gary W. Chemical Reviews, Vol. 115, Issue 23 https://doi.org/10.1021/acs.chemrev.5b00122	journal	July 2015
Histidine 332 of the D1 Polypeptide Modulates the Magnetic and Redox Properties of the Manganese Cluster and Tyrosine Y _Z in Photosystem II Debus, Richard J.; Campbell, Kristy A.; Peloquin, Jeffrey M. Biochemistry, Vol. 39, Issue 2 https://doi.org/10.1021/bi9917737	journal	December 1999
The nonheme iron in photosystem II Müh, Frank; Zouni, Athina Photosynthesis Research, Vol. 116, Issue 2-3 https://doi.org/10.1007/s11120-013-9926-y	journal	September 2013
Ligation of D1-His332 and D1-Asp170 to the Manganese Cluster of Photosystem II from Synechocystis Assessed by Multifrequency Pulse EPR Spectroscopy ^† Stich, Troy A.; Yeagle, Gregory J.; Service, Rachel J. Biochemistry, Vol. 50, Issue 34 https://doi.org/10.1021/bi2010703	journal	August 2011

Similar Records

D1-S169A Substitution of Photosystem II Perturbs Water Oxidation

Journal Article · Fri Feb 01 00:00:00 EST 2019 · Biochemistry · OSTI ID:1833470

Ghosh, Ipsita; Banerjee, Gourab; Kim, Christopher J.; +4 more

Substitution of the D1-Asn87 site in photosystem II of cyanobacteria mimics the chloride-binding characteristics of spinach photosystem II

Journal Article · Thu Feb 01 00:00:00 EST 2018 · Journal of Biological Chemistry · OSTI ID:1833470

Banerjee, Gourab; Ghosh, Ipsita; Kim, Christopher J.; +2 more

The O ₂ -Evolving Complex of Photosystem II: Recent Insights from Quantum Mechanics/Molecular Mechanics (QM/MM), Extended X-ray Absorption Fine Structure (EXAFS), and Femtosecond X-ray Crystallography Data

Journal Article · Wed Dec 21 00:00:00 EST 2016 · Accounts of Chemical Research · OSTI ID:1833470

Askerka, Mikhail; Brudvig, Gary W.; Batista, Victor S.

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Cyanobacteria
Decay kinetics
Mutation
Oxygen evolution
Photosystem II
Structure

Title: D1-S169A substitution of photosystem II reveals a novel S2-state structure

Citation Formats

References (69)

Similar Records

Related Subjects