D1-S169A Substitution of Photosystem II Perturbs Water Oxidation

Ghosh, Ipsita; Banerjee, Gourab; Kim, Christopher J.; Reiss, Krystle; Batista, Victor S.; Debus, Richard J.; Brudvig, Gary W.

doi:10.1021/acs.biochem.8b01184

D1-S169A Substitution of Photosystem II Perturbs Water Oxidation

Journal Article · Fri Feb 01 00:00:00 EST 2019 · Biochemistry

DOI:https://doi.org/10.1021/acs.biochem.8b01184· OSTI ID:1530435

Ghosh, Ipsita ^[1]; Banerjee, Gourab ^[1]; Kim, Christopher J. ^[2]; Reiss, Krystle ^[1]; ^[1]; ^[2]; ^[1]

Yale Univ., New Haven, CT (United States). Dept. of Chemistry
Univ. of California, Riverside, CA (United States). Dept. of Biochemistry

In photosystem II (PSII), photosynthetic water oxidation occurs at the tetramanganese–calcium cluster that cycles through light-induced intermediates (S₀–S₄) to produce oxygen from two substrate waters. The surrounding hydrogen-bonded amino acid residues and waters form channels that facilitate proton transfer and substrate water delivery, thereby ensuring efficient water oxidation. The residue D1-S169 lies in the “narrow” channel and forms hydrogen bonds with the Mn₄CaO₅ cluster via waters W1 and Wx. To probe the role of the narrow channel in substrate-water binding, we studied the D1-S169A mutation. PSII core complexes isolated from mutant cells exhibit inefficient S-state cycling and delayed oxygen evolution. The S₂-state multiline EPR spectrum of D1-S169A PSII core complexes differed significantly from that of wild-type, and FTIR difference spectra showed that the mutation strongly perturbs the extensive network of hydrogen bonds that extends at least from D1-Y161 (Y_Z) to D1-D61. Finally, these results imply a possible role of D1-S169 in proton egress or substrate water delivery.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES) (SC-22)

Grant/Contract Number:: FG02-05ER15646; SC0001423; SC0005291

OSTI ID:: 1530435

Journal Information:: Biochemistry, Journal Name: Biochemistry Journal Issue: 10 Vol. 58; ISSN 0006-2960

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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