Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue

Cho, Jin-Seong; Kim, Min-Ha; Bae, Eun-Kyung; Choi, Young-Im; Jeon, Hyung-Woo; Han, Kyung-Hwan; Ko, Jae-Heung

doi:10.1186/s13068-021-02029-2

Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue

Journal Article · Tue Sep 07 00:00:00 EDT 2021 · Biotechnology for Biofuels

DOI:https://doi.org/10.1186/s13068-021-02029-2· OSTI ID:1827191

Cho, Jin-Seong ^[1]; Kim, Min-Ha ^[2]; Bae, Eun-Kyung ^[3]; Choi, Young-Im ^[3]; Jeon, Hyung-Woo ^[2]; Han, Kyung-Hwan ^[4]; ^[2]

Kyung Hee University, Yongin (Korea, Republic of); Abio Materials Co., Ltd, Cheonan (Korea, Republic of); Michigan State University Department of Horticulture
Kyung Hee University, Yongin (Korea, Republic of)
National Institute of Forest Science, Suwon (Korea, Republic of)
Michigan State Univ., East Lansing, MI (United States)

Background: To create an ideotype woody bioenergy crop with desirable growth and biomass properties, we utilized the viral 2A-meidated bicistronic expression strategy to express both PtrMYB3 (MYB46 ortholog of Populus trichocarpa, a master regulator of secondary wall biosynthesis) and PdGA20ox1 (a GA20-oxidase from Pinus densiflora that produces gibberellins) in wood-forming tissue (i.e., developing xylem). Results: Transgenic Arabidopsis plants expressing the gene construct DX15::PdGA20ox1-2A-PtrMYB3 showed a significant increase in both stem fresh weight (threefold) and secondary wall thickening (1.27-fold) relative to wild-type (WT) plants. Transgenic poplars harboring the same gene construct grown in a greenhouse for 60 days had a stem fresh weight up to 2.6-fold greater than that of WT plants. In a living modified organism (LMO) field test conducted for 3 months of active growing season, the stem height and diameter growth of the transgenic poplars were 1.7- and 1.6-fold higher than those of WT plants, respectively, with minimal adverse growth defects. Although no significant changes in secondary wall thickening of the stem tissue of the transgenic poplars were observed, cellulose content was increased up to 14.4 wt% compared to WT, resulting in improved saccharification efficiency of the transgenic poplars. Moreover, enhanced woody biomass production by the transgenic poplars was further validated by re-planting in the same LMO field for additional two growing seasons. Conclusions: Taken together, these results show considerably enhanced wood formation of our transgenic poplars, with improved wood quality for biofuel production.

View Accepted Manuscript (DOE)

Research Organization:: Great Lakes Bioenergy Research Center (GLBRC), Madison, WI (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER); Forest Resources Genome Project; National Research Foundation of Korea (NRF)

Grant/Contract Number:: SC0018409

OSTI ID:: 1827191

Journal Information:: Biotechnology for Biofuels, Journal Name: Biotechnology for Biofuels Journal Issue: 1 Vol. 14; ISSN 1754-6834

Publisher:: BioMed CentralCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 1 of Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue Cho, Jin-Seong; Kim, Min-Ha; Bae, Eun-Kyung https://doi.org/10.6084/m9.figshare.16584584 The current record is supplemented by this text	text	January 2021
Additional file 1 of Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue Cho, Jin-Seong; Kim, Min-Ha; Bae, Eun-Kyung https://doi.org/10.6084/m9.figshare.16584584.v1 The current record is supplemented by this text	text	January 2021
Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue Cho, Jin-Seong; Kim, Min-Ha; Bae, Eun-Kyung https://doi.org/10.6084/m9.figshare.c.5608289 The current record is supplemented by this collection	collection	January 2021
Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue Cho, Jin-Seong; Kim, Min-Ha; Bae, Eun-Kyung https://doi.org/10.6084/m9.figshare.c.5608289.v1 The current record is supplemented by this collection	collection	January 2021

Field evaluation of transgenic hybrid poplars with desirable wood properties and enhanced growth for biofuel production by bicistronic expression of PdGA20ox1 and PtrMYB3 in wood-forming tissue

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