EliteTree™: an advanced biomass tree crop technology that features greater wood density and accelerated stem growth

Ko, Jae‐Heung; Kim, Won‐Chan; Cho, Jin‐Seong; Choi, Young‐Im; Park, Eung‐Jun; Im, Jong Hee; Han, Okran; Keathley, Daniel; Han, Kyung‐Hwan

doi:10.1002/bbb.1759

Title: EliteTree™: an advanced biomass tree crop technology that features greater wood density and accelerated stem growth

Journal Article · Wed Feb 15 00:00:00 EST 2017 · Biofuels, Bioproducts & Biorefining

DOI:https://doi.org/10.1002/bbb.1759· OSTI ID:1533121

Ko, Jae‐Heung ^[1]; Kim, Won‐Chan ^[2]; Cho, Jin‐Seong ^[1]; Choi, Young‐Im ^[3]; Park, Eung‐Jun ^[4]; Im, Jong Hee ^[5]; Han, Okran ^[6]; Keathley, Daniel ^[7]; Han, Kyung‐Hwan ^[7]

Kyung Hee University, Yongin (Korea)
Kyungpook National Univ., Daegu (Korea)
Korea Forest Research Institute, Suwon (Korea)
Korea Forest Research Institute Suwon Republic of Korea
Michigan State Univ., East Lansing, MI (United States)
KOPESS Biomass Solutions LLC, Okemos, MI (United States)
Michigan State Univ., East Lansing, MI (United States); KOPESS Biomass Solutions LLC, Okemos, MI (United States)

Abstract Due to wood's potential for large‐scale commercial production of biofuels, a rapid increase in the use of wood as a source of energy is expected as policies promoting greater use of renewable energy are adopted globally. However, the economics of purpose‐grown tree feedstocks for energy show that these production systems are not financially viable without improvement in the base growth rate. Conventional breeding programs have produced willow and poplar clones that show potential for rapid growth, but current top‐performing clones do not grow fast enough for profitable biofuel production. Genetic manipulation of secondary wall biosynthesis is the most direct path to resolving this growth barrier. To that end, we developed an innovative biomass tree crop technology, EliteTree™, that results in greater wood density and accelerated growth of stems. This technology is built on overexpression of Gibberellin 20‐oxidase to increase plant stem growth in both height and diameter and increase lignocellulosic biomass accumulation through overexpression of the transcription factor MYB46, which is a master regulator for secondary wall biosynthesis. EliteTree™ technology uses 2A‐mediated bicistronic gene expression, with our proprietary utility promoter DX15, such that the genetic manipulation is limited to wood tissue, yielding transgenic poplars with xylem‐specific co‐expression of MYB46 and PdGA20ox1. The development of faster growing elite tree genotypes with increased wood density and growth rates will pave the way for truly sustainable and economically viable bioenergy/biofuel production using short rotation tree plantations, and ultimately will lessen carbon emissions and has the potential to revitalize rural economies. © 2017 Society of Chemical Industry and John Wiley & Sons, Ltd

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Research Organization:: KOPESS Biomass Solutions, LLC, Okemos, MI (United States)

Sponsoring Organization:: USDOE Office of Science (SC); Michigan Translational Research and Commercialization; Korea Forest Service; National Research Foundation of Korea (NRF)

Grant/Contract Number:: SC0015208; S111213L080110; NRF-2015R1D1A1A01060807; DESC0015208

OSTI ID:: 1533121

Alternate ID(s):: OSTI ID: 1493473

Journal Information:: Biofuels, Bioproducts & Biorefining, Vol. 11, Issue 3; ISSN 1932-104X

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Cited by: 7 works

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Wood forming tissue‐specific bicistronic expression of Pd GA 20ox1 and Ptr MYB 221 improves both the quality and quantity of woody biomass production in a hybrid poplar Cho, Jin‐Seong; Jeon, Hyung‐Woo; Kim, Min‐Ha Plant Biotechnology Journal, Vol. 17, Issue 6 https://doi.org/10.1111/pbi.13036	journal	December 2018
Wood Transcriptome Profiling Identifies Critical Pathway Genes of Secondary Wall Biosynthesis and Novel Regulators for Vascular Cambium Development in Populus Kim, Min-Ha; Cho, Jin-Seong; Jeon, Hyung-Woo Genes, Vol. 10, Issue 9 https://doi.org/10.3390/genes10090690	journal	September 2019

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59 BASIC BIOLOGICAL SCIENCES
biotechnology & applied microbiology
energy & fuels
biofuel
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feedstock
MYB46
secondary wall
woody biomass

Title: EliteTree™: an advanced biomass tree crop technology that features greater wood density and accelerated stem growth

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