Analysis of intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites

Prigozhin, Daniil M.; Krasileva, Ksenia V.

doi:10.1093/plcell/koab013

Analysis of intraspecies diversity reveals a subset of highly variable plant immune receptors and predicts their binding sites

Journal Article · Mon Jan 25 04:00:00 EST 2021 · Plant Cell

DOI:https://doi.org/10.1093/plcell/koab013· OSTI ID:1825905

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Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Univ. of California, Berkeley, CA (United States)

The evolution of recognition specificities by the immune system depends on the generation of receptor diversity and on connecting the binding of new antigens with the initiation of downstream signaling. In plant immunity, the innate Nucleotide-Binding Leucine-Rich Repeat (NLR) receptor family enables antigen binding and immune signaling. In this study, we surveyed the NLR complements of 62 ecotypes of Arabidopsis thaliana and 54 lines of Brachypodium distachyon and identified a limited number of NLR subfamilies that show high allelic diversity. We show that the predicted specificity-determining residues cluster on the surfaces of Leucine-Rich Repeat domains, but the locations of the clusters vary among NLR subfamilies. By comparing NLR phylogeny, allelic diversity, and known functions of the Arabidopsis NLRs, we formulate a hypothesis for the emergence of direct and indirect pathogen-sensing receptors and of the autoimmune NLRs. These findings reveal the recurring patterns of evolution of innate immunity and can inform NLR engineering efforts.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23); Gordon and Betty Moore Foundation; Foundation for Food and Agriculture Research

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1825905

Journal Information:: Plant Cell, Journal Name: Plant Cell Journal Issue: 4 Vol. 33; ISSN 1040-4651

Publisher:: American Society of Plant BiologistsCopyright Statement

Country of Publication:: United States

Language:: English

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