Innovative nanopore sequencing technology including a self-assembled porous membrane
Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.
- Research Organization:
- Sandia National Lab. (SNL-NM), Albuquerque, NM (United States); Univ. of New Mexico, Albuquerque, NM (United States)
- Sponsoring Organization:
- USDOE; US Army Research Office (ARO)
- DOE Contract Number:
- AC04-94AL85000; FG02-02ER15368; W911NF-08-1-0214; W911NF-11-00080
- Assignee:
- STC.UNM (Albuquerque, NM)
- Patent Number(s):
- 10,969,364
- Application Number:
- 15/896,843
- OSTI ID:
- 1823813
- Resource Relation:
- Patent File Date: 02/14/2018
- Country of Publication:
- United States
- Language:
- English
Similar Records
Ion Gating in Nanopore Electrode Arrays with Hierarchically Organized pH-Responsive Block Copolymer Membranes
Single Entity Electrochemistry in Nanopore Electrode Arrays: Ion Transport Meets Electron Transfer in Confined Geometries