Innovative nanopore sequencing technology including a self-assembled porous membrane

Brueck, Steven R. J.; Edwards, Jeremy Scott; Neumann, Alexander; Kuznetsova, Yuliya; Mendoza, Edgar A.; Brinker, C. Jeffrey

Title: Innovative nanopore sequencing technology including a self-assembled porous membrane

Patent · Tue Mar 27 00:00:00 EDT 2018

OSTI ID:1532065

Brueck, Steven R. J.; Edwards, Jeremy Scott; Neumann, Alexander; Kuznetsova, Yuliya; Mendoza, Edgar A.; Brinker, C. Jeffrey

Methods and apparatus for long read, label-free, optical nanopore long chain molecule sequencing. In general, the present disclosure describes a novel sequencing technology based on the integration of nanochannels to deliver single long-chain molecules with widely spaced (>wavelength), ˜1-nm aperture “tortuous” nanopores that slow translocation sufficiently to provide massively parallel, single base resolution using optical techniques. A novel, directed self-assembly nanofabrication scheme using simple colloidal nanoparticles is used to form the nanopore arrays atop nanochannels that unfold the long chain molecules. At the surface of the nanoparticle array, strongly localized electromagnetic fields in engineered plasmonic/polaritonic structures allow for single base resolution using optical techniques.

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Research Organization:: Sandia National Lab. (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC04-94AL85000; FG02-02ER15368; W911NF-08-1-0214; W911NF-11-00080

Assignee:: STC.UNM (Albuquerque, NM)

Patent Number(s):: 9,927,397

Application Number:: 14/555,453

OSTI ID:: 1532065

Resource Relation:: Patent File Date: 2014-11-26

Country of Publication:: United States

Language:: English

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