Airy light-sheet Raman imaging

Subedi, N. R.; Yaraghi, S.; Jung, P. S.; Kukal, G.; McDonald, A. G.; Christodoulides, D. N.; Vasdekis, A. E.

doi:10.1364/OE.435293

Airy light-sheet Raman imaging

Journal Article · Tue Sep 21 00:00:00 EDT 2021 · Optics Express

DOI:https://doi.org/10.1364/OE.435293· OSTI ID:1821772

Subedi, N. R.; Yaraghi, S.; Jung, P. S.; Kukal, G.; McDonald, A. G.; Christodoulides, D. N.; Vasdekis, A. E.

Light-sheet fluorescence microscopy has greatly improved the speed and overall photostability of optically sectioning cellular and multi-cellular specimens. Similar gains have also been conferred by light-sheet Raman imaging; these schemes, however, rely on diffraction limited Gaussian beams that hinder the uniformity and size of the imaging field-of-view, and, as such, the resulting throughput rates. Here, we demonstrate that a digitally scanned Airy beam increases the Raman imaging throughput rates by more than an order of magnitude than conventional diffraction-limited beams. Overall, this, spectrometer-less, approach enabled 3D imaging of microparticles with high contrast and 1 µm axial resolution at 300 msec integration times per plane and orders of magnitude lower irradiation density than coherent Raman imaging schemes. We detail the apparatus and its performance, as well as its compatibility with fluorescence light-sheet and quantitative-phase imaging towards rapid and low phototoxicity multimodal imaging.

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Research Organization:: Univ. of Idaho, Moscow, ID (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019249

OSTI ID:: 1821772

Alternate ID(s):: OSTI ID: 1818773

Journal Information:: Optics Express, Journal Name: Optics Express Journal Issue: 20 Vol. 29; ISSN 1094-4087; ISSN OPEXFF

Publisher:: Optical Society of AmericaCopyright Statement

Country of Publication:: United States

Language:: English

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