Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol

Rodriguez, Alberto; Meadows, Jamie A.; Sun, Ning; Simmons, Blake A.; Gladden, John M.

doi:10.1186/s12934-021-01670-8

Evaluation of bacterial hosts for conversion of lignin-derived p-coumaric acid to 4-vinylphenol

Journal Article · Wed Sep 15 00:00:00 EDT 2021 · Microbial Cell Factories

DOI:https://doi.org/10.1186/s12934-021-01670-8· OSTI ID:1820204

Rodriguez, Alberto; Meadows, Jamie A.; Sun, Ning; Simmons, Blake A.;

Abstract

Hydroxycinnamic acids such as p -coumaric acid (CA) are chemically linked to lignin in grassy biomass with fairly labile ester bonds and therefore represent a straightforward opportunity to extract and valorize lignin components. In this work, we investigated the enzymatic conversion of CA extracted from lignocellulose to 4-vinylphenol (4VP) by expressing a microbial phenolic acid decarboxylase in Corynebacterium glutamicum , Escherichia coli, and Bacillus subtilis . The performance of the recombinant strains was evaluated in response to the substrate concentration in rich medium or a lignin liquor and the addition of an organic overlay to perform a continuous product extraction in batch cultures. We found that using undecanol as an overlay enhanced the 4VP titers under high substrate concentrations, while extracting > 97% of the product from the aqueous phase. C. glutamicum showed the highest tolerance to CA and resulted in the accumulation of up to 187 g/L of 4VP from pure CA in the overlay with a 90% yield when using rich media, or 17 g/L of 4VP with a 73% yield from CA extracted from lignin. These results indicate that C. glutamicum is a suitable host for the high-level production of 4VP and that further bioprocess engineering strategies should be explored to optimize the production, extraction, and purification of 4VP from lignin with this organism.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1820204

Alternate ID(s):: OSTI ID: 1825013
OSTI ID: 1828017

Journal Information:: Microbial Cell Factories, Journal Name: Microbial Cell Factories Journal Issue: 1 Vol. 20; ISSN 1475-2859

Publisher:: Springer Science + Business MediaCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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