Corynebacterium glutamicum as an Efficient Omnivorous Microbial Host for the Bioconversion of Lignocellulosic Biomass

Mhatre, Apurv; Shinde, Somnath; Jha, Amit Kumar; Rodriguez, Alberto; Wardak, Zohal; Jansen, Abigail; Gladden, John M.; George, Anthe; Davis, Ryan W.; Varman, Arul M.

doi:10.3389/fbioe.2022.827386

Corynebacterium glutamicum as an Efficient Omnivorous Microbial Host for the Bioconversion of Lignocellulosic Biomass

Journal Article · Fri Apr 01 00:00:00 EDT 2022 · Frontiers in Bioengineering and Biotechnology

DOI:https://doi.org/10.3389/fbioe.2022.827386· OSTI ID:1860507

Mhatre, Apurv; Shinde, Somnath; Jha, Amit Kumar; Rodriguez, Alberto; Wardak, Zohal; Jansen, Abigail; Gladden, John M.; George, Anthe; Davis, Ryan W.; Varman, Arul M.

Corynebacterium glutamicum has been successfully employed for the industrial production of amino acids and other bioproducts, partially due to its native ability to utilize a wide range of carbon substrates. We demonstrated C. glutamicum as an efficient microbial host for utilizing diverse carbon substrates present in biomass hydrolysates, such as glucose, arabinose, and xylose, in addition to its natural ability to assimilate lignin-derived aromatics. As a case study to demonstrate its bioproduction capabilities, L-lactate was chosen as the primary fermentation end product along with acetate and succinate. C. glutamicum was found to grow well in different aromatics (benzoic acid, cinnamic acid, vanillic acid, and p-coumaric acid) up to a concentration of 40 mM. Besides, ¹³ C-fingerprinting confirmed that carbon from aromatics enter the primary metabolism via TCA cycle confirming the presence of β-ketoadipate pathway in C. glutamicum . ¹³ C-fingerprinting in the presence of both glucose and aromatics also revealed coumarate to be the most preferred aromatic by C. glutamicum contributing 74 and 59% of its carbon for the synthesis of glutamate and aspartate respectively. ¹³ C-fingerprinting also confirmed the activity of ortho-cleavage pathway, anaplerotic pathway, and cataplerotic pathways. Finally, the engineered C. glutamicum strain grew well in biomass hydrolysate containing pentose and hexose sugars and produced L-lactate at a concentration of 47.9 g/L and a yield of 0.639 g/g from sugars with simultaneous utilization of aromatics. Succinate and acetate co-products were produced at concentrations of 8.9 g/L and 3.2 g/L, respectively. Our findings open the door to valorize all the major carbon components of biomass hydrolysate by using C. glutamicum as a microbial host for biomanufacturing.

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Research Organization:: Sandia National Laboratories (SNL-NM), Albuquerque, NM (United States)

Sponsoring Organization:: USDOE; USDOE Office of Energy Efficiency and Renewable Energy (EERE)

Grant/Contract Number:: NA0003525

OSTI ID:: 1860507

Alternate ID(s):: OSTI ID: 1872023

Report Number(s):: SAND2022-5102J; 827386

Journal Information:: Frontiers in Bioengineering and Biotechnology, Journal Name: Frontiers in Bioengineering and Biotechnology Vol. 10; ISSN 2296-4185

Publisher:: Frontiers Media SACopyright Statement

Country of Publication:: Switzerland

Language:: English

References (81)

Succinate production in Escherichia coli Thakker, Chandresh; Martínez, Irene; San, Ka-Yiu Biotechnology Journal, Vol. 7, Issue 2 https://doi.org/10.1002/biot.201100061	journal	September 2011
New tools for mass isotopomer data evaluation in13C flux analysis: Mass isotope correction, data consistency checking, and precursor relationships Wahl, S. Aljoscha; Dauner, Michael; Wiechert, Wolfgang Biotechnology and Bioengineering, Vol. 85, Issue 3 https://doi.org/10.1002/bit.10909	journal	January 2004
The Corynebacterium glutamicum genome: features and impacts on biotechnological processes Ikeda, M.; Nakagawa, S. Applied Microbiology and Biotechnology, Vol. 62, Issue 2-3 https://doi.org/10.1007/s00253-003-1328-1	journal	August 2003
Engineering of an l-arabinose metabolic pathway in Corynebacterium glutamicum Kawaguchi, Hideo; Sasaki, Miho; Vertès, Alain A. Applied Microbiology and Biotechnology, Vol. 77, Issue 5 https://doi.org/10.1007/s00253-007-1244-x	journal	October 2007
Production of d-lactic acid by Corynebacterium glutamicum under oxygen deprivation Okino, Shohei; Suda, Masako; Fujikura, Keitaro Applied Microbiology and Biotechnology, Vol. 78, Issue 3 https://doi.org/10.1007/s00253-007-1336-7	journal	March 2008
Engineering of pentose transport in Corynebacterium glutamicum to improve simultaneous utilization of mixed sugars Sasaki, Miho; Jojima, Toru; Kawaguchi, Hideo Applied Microbiology and Biotechnology, Vol. 85, Issue 1 https://doi.org/10.1007/s00253-009-2065-x	journal	June 2009
Engineering Corynebacterium glutamicum for the production of pyruvate Wieschalka, Stefan; Blombach, Bastian; Eikmanns, Bernhard J. Applied Microbiology and Biotechnology, Vol. 94, Issue 2, p. 449-459 https://doi.org/10.1007/s00253-011-3843-9	journal	January 2012
Degradation and assimilation of aromatic compounds by Corynebacterium glutamicum: another potential for applications for this bacterium? Shen, Xi-Hui; Zhou, Ning-Yi; Liu, Shuang-Jiang Applied Microbiology and Biotechnology, Vol. 95, Issue 1 https://doi.org/10.1007/s00253-012-4139-4	journal	May 2012
Detoxification of furfural in Corynebacterium glutamicum under aerobic and anaerobic conditions Tsuge, Yota; Hori, Yoshimi; Kudou, Motonori Applied Microbiology and Biotechnology, Vol. 98, Issue 20 https://doi.org/10.1007/s00253-014-5924-z	journal	August 2014
Identification of the phd gene cluster responsible for phenylpropanoid utilization in Corynebacterium glutamicum Kallscheuer, Nicolai; Vogt, Michael; Kappelmann, Jannick Applied Microbiology and Biotechnology, Vol. 100, Issue 4 https://doi.org/10.1007/s00253-015-7165-1	journal	November 2015
Conversion of lignin model compounds by Pseudomonas putida KT2440 and isolates from compost Ravi, Krithika; García-Hidalgo, Javier; Gorwa-Grauslund, Marie F. Applied Microbiology and Biotechnology, Vol. 101, Issue 12 https://doi.org/10.1007/s00253-017-8211-y	journal	March 2017
Metabolic engineering of Corynebacterium glutamicum for hyperproduction of polymer-grade l- and d-lactic acid Tsuge, Yota; Kato, Naoto; Yamamoto, Shogo Applied Microbiology and Biotechnology, Vol. 103, Issue 8 https://doi.org/10.1007/s00253-019-09737-8	journal	March 2019
Improved glucose and xylose co-utilization by overexpression of xylose isomerase and/or xylulokinase genes in oleaginous fungus Mucor circinelloides Zan, Xinyi; Sun, Jianing; Chu, Linfang Applied Microbiology and Biotechnology, Vol. 105, Issue 13 https://doi.org/10.1007/s00253-021-11392-x	journal	July 2021
A heat shock following electroporation induces highly efficient transformation of Corynebacterium glutamicum with xenogeneic plasmid DNA van der Rest, M. E.; Lange, C.; Molenaar, D. Applied Microbiology and Biotechnology, Vol. 52, Issue 4 https://doi.org/10.1007/s002530051557	journal	October 1999
Hemicellulose bioconversion Saha, Badal C. Journal of Industrial Microbiology and Biotechnology, Vol. 30, Issue 5 https://doi.org/10.1007/s10295-003-0049-x	journal	May 2003
Recent advances in the metabolic engineering of Corynebacterium glutamicum for the production of lactate and succinate from renewable resources Tsuge, Yota; Hasunuma, Tomohisa; Kondo, Akihiko Journal of Industrial Microbiology and Biotechnology, Vol. 42, Issue 3 https://doi.org/10.1007/s10295-014-1538-9	journal	March 2015
Central metabolism in Mycobacterium smegmatis during the transition from O2-rich to O2-poor conditions as studied by isotopomer-assisted metabolite analysis Tang, Yinjie J.; Shui, Wenqing; Myers, Samuel Biotechnology Letters, Vol. 31, Issue 8 https://doi.org/10.1007/s10529-009-9991-7	journal	April 2009
Engineering Escherichia coli to convert acetic acid to free fatty acids Xiao, Yi; Ruan, Zhenhua; Liu, Zhiguo Biochemical Engineering Journal, Vol. 76 https://doi.org/10.1016/j.bej.2013.04.013	journal	July 2013
Effect of hemicellulose and lignin removal on enzymatic hydrolysis of steam pretreated corn stover Ohgren, K.; Bura, R.; Saddler, J. Bioresource Technology, Vol. 98, Issue 13 https://doi.org/10.1016/j.biortech.2006.09.003	journal	September 2007
Hemicelluloses for fuel ethanol: A review Gírio, F. M.; Fonseca, C.; Carvalheiro, F. Bioresource Technology, Vol. 101, Issue 13 https://doi.org/10.1016/j.biortech.2010.01.088	journal	July 2010
Efficient production of d-(−)-lactic acid from broken rice by Lactobacillus delbrueckii using Ca(OH)2 as a neutralizing agent Nakano, Shinta; Ugwu, Charles U.; Tokiwa, Yutaka Bioresource Technology, Vol. 104 https://doi.org/10.1016/j.biortech.2011.10.017	journal	January 2012
Pilot scale demonstration of d -lactic acid fermentation facilitated by Ca(OH) 2 using a metabolically engineered Escherichia coli Liu, Ye; Gao, Wa; Zhao, Xiao Bioresource Technology, Vol. 169 https://doi.org/10.1016/j.biortech.2014.06.056	journal	October 2014
Efficient simultaneous utilization of glucose and xylose from corn straw by Sphingomonas sanxanigenens NX02 to produce microbial exopolysaccharide Wu, Mengmeng; Zhao, Xin; Shen, Yaqi Bioresource Technology, Vol. 319 https://doi.org/10.1016/j.biortech.2020.124126	journal	January 2021
Fed-batch fermentation for enhanced lactic acid production from glucose/xylose mixture without carbon catabolite repression Abdel-Rahman, Mohamed Ali; Xiao, Yaotian; Tashiro, Yukihiro Journal of Bioscience and Bioengineering, Vol. 119, Issue 2 https://doi.org/10.1016/j.jbiosc.2014.07.007	journal	February 2015
Enhanced production of d-lactate from mixed sugars in Corynebacterium glutamicum by overexpression of glycolytic genes encoding phosphofructokinase and triosephosphate isomerase Tsuge, Yota; Kato, Naoto; Yamamoto, Shogo Journal of Bioscience and Bioengineering, Vol. 127, Issue 3 https://doi.org/10.1016/j.jbiosc.2018.08.002	journal	March 2019
Production of the amino acids l-glutamate, l-lysine, l-ornithine and l-arginine from arabinose by recombinant Corynebacterium glutamicum Schneider, Jens; Niermann, Karin; Wendisch, Volker F. Journal of Biotechnology, Vol. 154, Issue 2-3 https://doi.org/10.1016/j.jbiotec.2010.07.009	journal	July 2011
Batch and continuous separation of acetic acid from succinic acid in a feed solution with high concentrations of carboxylic acids by emulsion liquid membranes Lee, Sang Cheol; Kim, Heon Chang Journal of Membrane Science, Vol. 367, Issue 1-2 https://doi.org/10.1016/j.memsci.2010.10.057	journal	February 2011
Lignin biorefinery: Separation of vanillin, vanillic acid and acetovanillone by adsorption Gomes, E. D.; Rodrigues, A. E. Separation and Purification Technology, Vol. 216 https://doi.org/10.1016/j.seppur.2019.01.071	journal	June 2019
Metabolic engineering as a tool for enhanced lactic acid production Upadhyaya, Bikram P.; DeVeaux, Linda C.; Christopher, Lew P. Trends in Biotechnology, Vol. 32, Issue 12 https://doi.org/10.1016/j.tibtech.2014.10.005	journal	December 2014
From lignin to nylon: Cascaded chemical and biochemical conversion using metabolically engineered Pseudomonas putida Kohlstedt, Michael; Starck, Sören; Barton, Nadja Metabolic Engineering, Vol. 47 https://doi.org/10.1016/j.ymben.2018.03.003	journal	May 2018
A coculture-coproduction system designed for enhanced carbon conservation through inter-strain CO2 recycling Flores, Andrew D.; Holland, Steven C.; Mhatre, Apurv Metabolic Engineering, Vol. 67 https://doi.org/10.1016/j.ymben.2021.08.001	journal	September 2021
The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of l-aspartate-derived amino acids and vitamins Kalinowski, Jörn; Bathe, Brigitte; Bartels, Daniela Journal of Biotechnology, Vol. 104, Issue 1-3 https://doi.org/10.1016/s0168-1656(03)00154-8	journal	September 2003
Deacetylation and Mechanical Refining (DMR) and Deacetylation and Dilute Acid (DDA) Pretreatment of Corn Stover, Switchgrass, and a 50:50 Corn Stover/Switchgrass Blend Kuhn, Erik M.; Chen, Xiaowen; Tucker, Melvin P. ACS Sustainable Chemistry & Engineering, Vol. 8, Issue 17 https://doi.org/10.1021/acssuschemeng.0c00894	journal	April 2020
Muconic Acid Production Using Engineered Pseudomonas putida KT2440 and a Guaiacol-Rich Fraction Derived from Kraft Lignin Almqvist, Henrik; Veras, Henrique; Li, Kena ACS Sustainable Chemistry & Engineering, Vol. 9, Issue 24 https://doi.org/10.1021/acssuschemeng.1c00933	journal	June 2021
Base-Catalyzed Depolymerization of Solid Lignin-Rich Streams Enables Microbial Conversion Rodriguez, Alberto; Salvachúa, Davinia; Katahira, Rui ACS Sustainable Chemistry & Engineering, Vol. 5, Issue 9 https://doi.org/10.1021/acssuschemeng.7b01818	journal	August 2017
Production of Biocompatible and Antimicrobial Bacterial Cellulose Polymers Functionalized by RGDC Grafting Groups and Gentamicin Rouabhia, Mahmoud; Asselin, Jérémie; Tazi, Neftaha ACS Applied Materials & Interfaces, Vol. 6, Issue 3 https://doi.org/10.1021/am4027983	journal	January 2014
Kinetic Modeling and Isotopic Investigation of Isobutanol Fermentation by Two Engineered Escherichia coli Strains Xiao, Yi; Feng, Xueyang; Varman, Arul M. Industrial & Engineering Chemistry Research, Vol. 51, Issue 49 https://doi.org/10.1021/ie202936t	journal	November 2012
Structure, Energy, Synergy, TimeThe Fundamentals of Process Intensification Van Gerven, Tom; Stankiewicz, Andrzej Industrial & Engineering Chemistry Research, Vol. 48, Issue 5 https://doi.org/10.1021/ie801501y	journal	March 2009
Separation of Acetic Acid, Formic Acid, Succinic Acid, and Lactic Acid Using Adsorbent Resin Nam, Hee-Geun; Lim, Geon-Woo; Mun, Sungyong Journal of Chemical & Engineering Data, Vol. 57, Issue 8 https://doi.org/10.1021/je201065u	journal	July 2012
Biomass conversion: Lignin up for break-down Bruijnincx, Pieter C. A.; Weckhuysen, Bert M. Nature Chemistry, Vol. 6, Issue 12, p. 1035-1036 https://doi.org/10.1038/nchem.2120	journal	December 2014
Direct hydrodeoxygenation of raw woody biomass into liquid alkanes Xia, Qineng; Chen, Zongjia; Shao, Yi Nature Communications, Vol. 7, Issue 1 https://doi.org/10.1038/ncomms11162	journal	March 2016
Lignin Valorization: Two Hybrid Biochemical Routes for the Conversion of Polymeric Lignin into Value-added Chemicals Wu, Weihua; Dutta, Tanmoy; Varman, Arul M. Scientific Reports, Vol. 7, Issue 1 https://doi.org/10.1038/s41598-017-07895-1	journal	August 2017
Fermentation of sugar mixtures using Escherichia coli catabolite repression mutants engineered for production of L-lactic acid Dien, B. S.; Nichols, N. N.; Bothast, R. J. Journal of Industrial Microbiology and Biotechnology, Vol. 29, Issue 5, p. 221-227 https://doi.org/10.1038/sj.jim.7000299	journal	November 2002
Functional characterization of a vanillin dehydrogenase in Corynebacterium glutamicum Ding, Wei; Si, Meiru; Zhang, Weipeng Scientific Reports, Vol. 5, Issue 1 https://doi.org/10.1038/srep08044	journal	January 2015
DMR (deacetylation and mechanical refining) processing of corn stover achieves high monomeric sugar concentrations (230 g L ⁻¹ ) during enzymatic hydrolysis and high ethanol concentrations (>10% v/v) during fermentation without hydrolysate purification or concentration Chen, Xiaowen; Kuhn, Erik; Jennings, Edward W. Energy & Environmental Science, Vol. 9, Issue 4 https://doi.org/10.1039/C5EE03718B	journal	January 2016
Chemoselective catalytic conversion of glycerol as a biorenewable source to valuable commodity chemicals Zhou, Chun-Hui (Clayton); Beltramini, Jorge N.; Fan, Yong-Xian Chem. Soc. Rev., Vol. 37, Issue 3 https://doi.org/10.1039/b707343g	journal	January 2008
Catalytic conversion of lignocellulosic biomass to fine chemicals and fuels Zhou, Chun-Hui; Xia, Xi; Lin, Chun-Xiang Chemical Society Reviews, Vol. 40, Issue 11 https://doi.org/10.1039/c1cs15124j	journal	January 2011
Towards lignin consolidated bioprocessing: simultaneous lignin depolymerization and product generation by bacteria Salvachúa, Davinia; Karp, Eric M.; Nimlos, Claire T. Green Chemistry, Vol. 17, Issue 11 https://doi.org/10.1039/c5gc01165e	journal	January 2015
Efficient biomass pretreatment using ionic liquids derived from lignin and hemicellulose Socha, A. M.; Parthasarathi, R.; Shi, J. Proceedings of the National Academy of Sciences, Vol. 111, Issue 35 https://doi.org/10.1073/pnas.1405685111	journal	August 2014
Lignin valorization through integrated biological funneling and chemical catalysis Linger, J. G.; Vardon, D. R.; Guarnieri, M. T. Proceedings of the National Academy of Sciences, Vol. 111, Issue 33, p. 12013-12018 https://doi.org/10.1073/pnas.1410657111	journal	August 2014
Decoding how a soil bacterium extracts building blocks and metabolic energy from ligninolysis provides road map for lignin valorization Varman, Arul M.; He, Lian; Follenfant, Rhiannon Proceedings of the National Academy of Sciences, Vol. 113, Issue 40 https://doi.org/10.1073/pnas.1606043113	journal	September 2016
Experimental evolution reveals an effective avenue to release catabolite repression via mutations in XylR Sievert, Christian; Nieves, Lizbeth M.; Panyon, Larry A. Proceedings of the National Academy of Sciences, Vol. 114, Issue 28 https://doi.org/10.1073/pnas.1700345114	journal	June 2017
Comparative analysis of the Corynebacterium glutamicum group and complete genome sequence of strain R Yukawa, Hideaki; Omumasaba, Crispinus A.; Nonaka, Hiroshi Microbiology, Vol. 153, Issue 4 https://doi.org/10.1099/mic.0.2006/003657-0	journal	April 2007
Accelerated pentose utilization by Corynebacterium glutamicum for accelerated production of lysine, glutamate, ornithine and putrescine: Accelerated pentose utilization by C. glutamicum Meiswinkel, Tobias M.; Gopinath, Vipin; Lindner, Steffen N. Microbial Biotechnology, Vol. 6, Issue 2 https://doi.org/10.1111/1751-7915.12001	journal	November 2012
Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin Salvachúa, Davinia; Rydzak, Thomas; Auwae, Raquel Microbial Biotechnology, Vol. 13, Issue 3 https://doi.org/10.1111/1751-7915.13547	journal	May 2020
Mode of antimicrobial action of vanillin against Escherichia coli, Lactobacillus plantarum and Listeria innocua Fitzgerald, D. J.; Stratford, M.; Gasson, M. J. Journal of Applied Microbiology, Vol. 97, Issue 1 https://doi.org/10.1111/j.1365-2672.2004.02275.x	journal	July 2004
Valorization of Biomass: Deriving More Value from Waste Tuck, C. O.; Perez, E.; Horvath, I. T. Science, Vol. 337, Issue 6095, p. 695-699 https://doi.org/10.1126/science.1218930	journal	August 2012
Homolactate Fermentation by Metabolically Engineered Escherichia coli Strains Zhu, Y.; Eiteman, M. A.; DeWitt, K. Applied and Environmental Microbiology, Vol. 73, Issue 2 https://doi.org/10.1128/aem.02022-06	journal	November 2006
Effect of Lignocellulose-Derived Inhibitors on Growth of and Ethanol Production by Growth-Arrested Corynebacterium glutamicum R Sakai, S.; Tsuchida, Y.; Okino, S. Applied and Environmental Microbiology, Vol. 73, Issue 7 https://doi.org/10.1128/aem.02880-06	journal	February 2007
Vanillin Catabolism in Rhodococcus jostii RHA1 Chen, Hao-Ping; Chow, Mindy; Liu, Chi-Chun Applied and Environmental Microbiology, Vol. 78, Issue 2 https://doi.org/10.1128/aem.06876-11	journal	November 2011
Engineering of a Xylose Metabolic Pathway in Corynebacterium glutamicum Kawaguchi, H.; Vertes, A. A.; Okino, S. Applied and Environmental Microbiology, Vol. 72, Issue 5 https://doi.org/10.1128/aem.72.5.3418-3428.2006	journal	May 2006
Genome Sequence of Corynebacterium glutamicum S9114, a Strain for Industrial Production of Glutamate Lv, Yangyong; Wu, Zhanhong; Han, Shuangyan Journal of Bacteriology, Vol. 193, Issue 21 https://doi.org/10.1128/jb.06074-11	journal	November 2011
Metabolic Analysis of Corynebacterium glutamicum during Lactate and Succinate Productions under Oxygen Deprivation Conditions Inui, Masayuki; Murakami, Shikiko; Okino, Shohei Journal of Molecular Microbiology and Biotechnology, Vol. 7, Issue 4 https://doi.org/10.1159/000079827	journal	January 2004
Functional genomics of pH homeostasis in Corynebacterium glutamicum revealed novel links between pH response, oxidative stress, iron homeostasis and methionine synthesis Follmann, Martin; Ochrombel, Ines; Krämer, Reinhard BMC Genomics, Vol. 10, Issue 1 https://doi.org/10.1186/1471-2164-10-621	journal	December 2009
Engineering Escherichia coli for succinate production from hemicellulose via consolidated bioprocessing Zheng, Zongbao; Chen, Tao; Zhao, Meina Microbial Cell Factories, Vol. 11, Issue 1 https://doi.org/10.1186/1475-2859-11-37	journal	January 2012
Photoautotrophic production of D-lactic acid in an engineered cyanobacterium Varman, Arul M.; Yu, Yi; You, Le Microbial Cell Factories, Vol. 12, Issue 1 https://doi.org/10.1186/1475-2859-12-117	journal	January 2013
Elucidation of intrinsic biosynthesis yields using 13C-based metabolism analysis Varman, Arul M.; He, Lian; You, Le Microbial Cell Factories, Vol. 13, Issue 1 https://doi.org/10.1186/1475-2859-13-42	journal	January 2014
Study on roles of anaplerotic pathways in glutamate overproduction of Corynebacterium glutamicum by metabolic flux analysis Shirai, Tomokazu; Fujimura, Koki; Furusawa, Chikara Microbial Cell Factories, Vol. 6, Issue 1 https://doi.org/10.1186/1475-2859-6-19	journal	June 2007
Next-generation biomass feedstocks for biofuel production Simmons, Blake A.; Loque, Dominique; Blanch, Harvey W. Genome Biology, Vol. 9, Issue 12 https://doi.org/10.1186/gb-2008-9-12-242	journal	January 2008
A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum Chai, Xin; Shang, Xiuling; Zhang, Yu BMC Biotechnology, Vol. 16, Issue 1 https://doi.org/10.1186/s12896-016-0313-6	journal	November 2016
High-yield anaerobic succinate production by strategically regulating multiple metabolic pathways based on stoichiometric maximum in Escherichia coli Meng, Jiao; Wang, Baiyun; Liu, Dingyu Microbial Cell Factories, Vol. 15, Issue 1 https://doi.org/10.1186/s12934-016-0536-1	journal	August 2016
Bioconversion of distillers’ grains hydrolysates to advanced biofuels by an Escherichia coli co-culture Liu, Fang; Wu, Weihua; Tran-Gyamfi, Mary B. Microbial Cell Factories, Vol. 16, Issue 1 https://doi.org/10.1186/s12934-017-0804-8	journal	November 2017
Metabolic engineering of Rhodococcus jostii RHA1 for production of pyridine-dicarboxylic acids from lignin Spence, Edward M.; Calvo-Bado, Leonides; Mines, Paul Microbial Cell Factories, Vol. 20, Issue 1 https://doi.org/10.1186/s12934-020-01504-z	journal	January 2021
Hybrid phenolic-inducible promoters towards construction of self-inducible systems for microbial lignin valorization Varman, Arul M.; Follenfant, Rhiannon; Liu, Fang Biotechnology for Biofuels, Vol. 11, Issue 1 https://doi.org/10.1186/s13068-018-1179-8	journal	June 2018
The chemical nature of phenolic compounds determines their toxicity and induces distinct physiological responses in Saccharomyces cerevisiae in lignocellulose hydrolysates Adeboye, Peter Temitope; Bettiga, Maurizio; Olsson, Lisbeth AMB Express, Vol. 4, Issue 1 https://doi.org/10.1186/s13568-014-0046-7	journal	May 2014
Genomic Analysis and Identification of Catabolic Pathways for Aromatic Compounds in Corynebacterium glutamicum Shen, Xi-Hui; Huang, Yan; Liu, Shuang-Jiang Microbes and Environments, Vol. 20, Issue 3 https://doi.org/10.1264/jsme2.20.160	journal	January 2005
A Perspective on Polylactic Acid-Based Polymers Use for Nanoparticles Synthesis and Applications Casalini, Tommaso; Rossi, Filippo; Castrovinci, Andrea Frontiers in Bioengineering and Biotechnology, Vol. 7 https://doi.org/10.3389/fbioe.2019.00259	journal	October 2019
Catabolic Division of Labor Enhances Production of D-Lactate and Succinate From Glucose-Xylose Mixtures in Engineered Escherichia coli Co-culture Systems Flores, Andrew D.; Choi, Hyun G.; Martinez, Rodrigo Frontiers in Bioengineering and Biotechnology, Vol. 8 https://doi.org/10.3389/fbioe.2020.00329	journal	May 2020
Efficient Co-Utilization of Biomass-Derived Mixed Sugars for Lactic Acid Production by Bacillus coagulans Azu-10 Abdel-Rahman, Mohamed Ali; Hassan, Saad El-Din; Alrefaey, Hassan M. A. Fermentation, Vol. 7, Issue 1 https://doi.org/10.3390/fermentation7010028	journal	February 2021
Metabolic Pathway Confirmation and Discovery Through ¹³C-labeling of Proteinogenic Amino Acids You, Le; Page, Lawrence; Feng, Xueyang Journal of Visualized Experiments, Issue 59 https://doi.org/10.3791/3583	journal	January 2012
Metabolic Engineering of Lactic acid Bacteria for the Production of Industrially Important Compounds Papagianni, Maria Computational and Structural Biotechnology Journal, Vol. 3, Issue 4 https://doi.org/10.5936/csbj.201210003	journal	October 2012

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