Peripheral blood hematology, plasma biochemistry, and the optimization of an in vitro immune-based assay in the brown watersnake (Nerodia taxispilota)

Haskins, David Lee; Brown, M. Kyle; Meichner, Kristina; Tuberville, Tracey D.; Gogal, Robert M.

doi:10.1080/15321819.2020.1808479

Peripheral blood hematology, plasma biochemistry, and the optimization of an in vitro immune-based assay in the brown watersnake (Nerodia taxispilota)

Journal Article · Tue Aug 18 00:00:00 EDT 2020 · Journal of Immunoassay & Immunochemistry

DOI:https://doi.org/10.1080/15321819.2020.1808479· OSTI ID:1819847

^[1]; Brown, M. Kyle ^[2]; Meichner, Kristina ^[3]; Tuberville, Tracey D. ^[4]; Gogal, Robert M. ^[5]

Univ. of Georgia, Athens, GA (United States); Univ. of Georgia, Aiken, SC (United States); Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL); SREL
Univ. of Georgia, Aiken, SC (United States); Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)
Univ. of Georgia College of Veterinary Medicine, Athens, GA (United States)
Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)
Univ. of Georgia, Athens, GA (United States)

Reptiles represent a phylogenetic lineage that provides a unique link between ectothermic anamniotes and endothermic amniotes. Compared to mammalian and avian species, our understanding of the reptilian immune system is greatly lacking. This gap in knowledge is largely due to an absence of established immune-based assays or specific reagents for these species. In the present study, brown watersnakes (Nerodia taxispilota) were live-captured in the wild, sexed, weighed, measured, bled via the caudal vein and released. At 24 hr post-collection, peripheral blood leukocytes were enriched and evaluated with an established mammalian in-vitro lymphocyte proliferation assay. Snake peripheral blood leukocyte enrichment yielded > 90% lymphocytes with viabilities averaging 81.5%. Baseline physiologic data for N. taxispilota, including hematology and total solids, leukocyte differentials, cell recovery and plasma biochemistry, were also collected. Cells cultured with Concanavalin A exhibited significantly increased proliferation at both 72 and 96 hr. Furthermore, these preliminary results show that enriched peripheral blood from wild-caught N. taxispilota provides a sufficient yield of leukocytes that can be cultured and functionally evaluated using a standard mammalian in-vitro immune-based assay.

View Accepted Manuscript (DOE)

Research Organization:: Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC09-07SR22506

OSTI ID:: 1819847

Journal Information:: Journal of Immunoassay & Immunochemistry, Journal Name: Journal of Immunoassay & Immunochemistry Journal Issue: 1 Vol. 42; ISSN 1532-1819

Publisher:: Taylor & FrancisCopyright Statement

Country of Publication:: United States

Language:: English

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Peripheral blood hematology, plasma biochemistry, and the optimization of an in vitro immune-based assay in the brown watersnake (Nerodia taxispilota)

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