Accumulation of coal combustion residues and their immunological effects in the yellow-bellied slider (Trachemys scripta scripta)

Journal Article · Wed Mar 08 00:00:00 EST 2017 · Environmental Pollution

DOI:https://doi.org/10.1016/j.envpol.2017.01.048· OSTI ID:1817856

Haskins, David L. ^[1]; Hamilton, Matthew T. ^[2]; Jones, Amanda L. ^[3]; Finger, Jr., John W. ^[4]; Bringolf, Robert B. ^[5]; Tuberville, Tracey D. ^[2]

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1. Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL); Univ. of Georgia, Athens, GA (United States); SREL
2. Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL); Univ. of Georgia, Athens, GA (United States)
3. Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)
4. Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL); Auburn Univ., AL (United States)
5. Univ. of Georgia, Athens, GA (United States)

Anthropogenic activities such as industrial processes often produce copious amounts of contaminants that have the potential to negatively impact growth, survival, and reproduction of exposed wildlife. Coal combustion residues (CCRs) represent a major source of pollutants globally, resulting in the release of potentially harmful trace elements such as arsenic (As), cadmium (Cd), and selenium (Se) into the environment. In the United States, CCRs are typically stored in aquatic settling basins that may become attractive nuisances to wildlife. Trace element contaminants, such as CCRs, may pose a threat to biota yet little is known about their sublethal effects on reptiles. To assess the effects of CCR exposure in turtles, we sampled 81 yellow-bellied sliders (Trachemys scripta scripta) in 2014-2015 from CCR-contaminated and uncontaminated reference wetlands located on the Savannah River Site (Aiken, SC, USA). Specific aims were to (1) compare the accumulation of trace elements in T. s. scripta claw and blood samples between reference and CCR-contaminated site types, (2) evaluate potential immunological effects of CCRs via bacterial killing assays and phytohaemagglutinin (PHA) assays, and (3) quantify differences in hemogregarine parasite loads between site types. Claw As, Cd, copper (Cu), and Se (all p ≤ 0.001) and blood As, Cu, Se, and strontium (Sr; p ≤ 0.015) were significantly elevated in turtles from CCR-contaminated wetlands compared to turtles from reference wetlands. Turtles from reference wetlands exhibited lower bacterial killing (p = 0.015) abilities than individuals from contaminated sites but neither PHA responses (p = 0.566) nor parasite loads (p = 0.980) differed by site type. Despite relatively high CCR body burdens, sliders did not exhibit apparent impairment of immunological response or parasite load. Additionally, the high correlation between claw and blood concentrations within individuals suggests that nonlethal tissue sampling may be useful for monitoring CCR exposure in turtles.

• Research Organization: Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)
• Sponsoring Organization: USDOE
• Grant/Contract Number: FC09-07SR22506
• OSTI ID: 1817856
• Alternate ID(s): OSTI ID: 1414331; OSTI ID: 22707170
• Journal Information: Environmental Pollution, Journal Name: Environmental Pollution Vol. 224; ISSN 0269-7491
• Publisher: Elsevier
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
Bioaccumulation
Coal combustion residues
Ecoimmunology
Nondestructive sampling
Reptile