Effects of selenium exposure on the hematology, innate immunity, and metabolic rate of yellow-bellied sliders (Trachemys scripta scripta)

Haskins, David L.; Hamilton, Matthew T.; Stacy, Nicole I.; Finger, Jr., John W.; Tuberville, Tracey D.

doi:10.1007/s10646-017-1839-7

Effects of selenium exposure on the hematology, innate immunity, and metabolic rate of yellow-bellied sliders (Trachemys scripta scripta)

Journal Article · Sun Aug 06 00:00:00 EDT 2017 · Ecotoxicology

DOI:https://doi.org/10.1007/s10646-017-1839-7· OSTI ID:1817663

^[1]; Hamilton, Matthew T. ^[2]; Stacy, Nicole I. ^[3]; Finger, Jr., John W. ^[4]; Tuberville, Tracey D. ^[5]

Univ. of Georgia, Aiken, SC (United States); Univ. of Georgia, Athens, GA (United States); SREL
Univ. of Georgia, Aiken, SC (United States); Univ. of Georgia, Athens, GA (United States)
Univ. of Florida, Gainesville, FL (United States)
Univ. of Georgia, Aiken, SC (United States); Auburn Univ., AL (United States)
Univ. of Georgia, Aiken, SC (United States)

Selenium (Se) is a naturally occurring essential element that can be toxic to vertebrates at high concentrations. Despite studies that have documented that wild reptile species can accumulate copious amounts of Se, little is known regarding specific toxicologic effects of Se. In this study, 70 juvenile yellow-bellied sliders (Trachemys scripta scripta) were exposed to one of three seleno-L-methionine (SetMet) treatments (control, n = 24; 15 mg/kg, n = 23; and 30 mg/kg, n = 23) via weekly oral gavage for 5 weeks. At the conclusion of the experiment, kidney, liver, muscle, and blood samples were collected for quantitative Se analysis. Turtles in the SeMet treatment groups accumulated significantly higher amounts of Se in all tissue types relative to controls (all p < 0.001). Turtles in the 30 mg/kg SeMet group also accumulated significantly higher amounts of Se compared to the 15 mg/kg group (all p < 0.001). Although toxicity thresholds for reptiles have not been established, Se concentrations in liver tissue from both SeMet treatment groups exceeded reported avian toxicity thresholds for liver tissue. Neither oxygen consumption nor innate bactericidal capacity were impacted by SeMet exposure. Furthermore, turtles in the 30 mg/kg SeMet group exhibited anemia, which has been reported in other vertebrates exposed to Se. Furthermore, juvenile T. s. scripta in the 30 mg/kg SeMet group experienced 17% mortality compared to 0% in the 15 mg/kg treatment and control groups. To our knowledge, this study is the first to report dose-dependent Se-associated anemia and mortality in a chelonian species.

View Accepted Manuscript (DOE)

Research Organization:: Savannah River Site (SRS), Aiken, SC (United States). Savannah River Ecology Lab. (SREL)

Sponsoring Organization:: USDOE

Grant/Contract Number:: FC09-07SR22506

OSTI ID:: 1817663

Alternate ID(s):: OSTI ID: 1537745

Journal Information:: Ecotoxicology, Journal Name: Ecotoxicology Journal Issue: 8 Vol. 26; ISSN 0963-9292

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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Experimentally Induced Selenosis in Yellow-Bellied Slider Turtles ( Trachemys scripta scripta ) Haskins, David L.; Howerth, Elizabeth W.; Tuberville, Tracey D. Veterinary Pathology, Vol. 55, Issue 3 https://doi.org/10.1177/0300985817750454	journal	January 2018

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59 BASIC BIOLOGICAL SCIENCES
Immune response
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Selenomethionine
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Slider

Effects of selenium exposure on the hematology, innate immunity, and metabolic rate of yellow-bellied sliders (Trachemys scripta scripta)

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References (49)

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