Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments

Wang, Sheng; Wong Po Foo, Cheryl; Warrier, Ajithkumar; Poo, Mu-ming; Heilshorn, Sarah C.; Zhang, Xiang

doi:10.1007/s10544-009-9329-1

Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments

Journal Article · Wed Jun 03 00:00:00 EDT 2009 · Biomedical Microdevices

DOI:https://doi.org/10.1007/s10544-009-9329-1· OSTI ID:1816134

Wang, Sheng ^[1]; Wong Po Foo, Cheryl ^[2]; Warrier, Ajithkumar ^[3]; Poo, Mu-ming ^[4]; Heilshorn, Sarah C. ^[2]; Zhang, Xiang ^[5]

Univ. of California, Berkeley, CA (United States). NSF Nanoscale Science and Engineering Center (NSEC); Univ. of California, Berkeley, CA (United States). Applied Science and Technology Graduate Program; OSTI
Stanford Univ., CA (United States). Dept. of Materials Science and Engineering
Univ. of California, Berkeley, CA (United States). NSF Nanoscale Science and Engineering Center (NSEC)
Stanford Univ., CA (United States). Dept. of Materials Science and Engineering; Univ. of California, Berkeley, CA (United States). Dept. of Molecular and Cell Biology
Univ. of California, Berkeley, CA (United States). NSF Nanoscale Science and Engineering Center (NSEC); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). Materials Sciences Division

Spatial patterning of proteins is a valuable technique for many biological applications and is the prevailing tool for defining microenvironments for cells in culture, a required procedure in developmental biology and tissue engineering research. However, it is still challenging to achieve protein patterns that closely mimic native microenvironments, such as gradient protein distributions with desirable mechanical properties. By combining projection dynamic mask lithography and protein engineering with non-canonical photosensitive amino acids, we demonstrate a simple, scalable strategy to fabricate any user-defined 2D or 3D stable gradient pattern with complex geometries from an artificial extracellular matrix (aECM) protein. We show that the elastic modulus and chemical nature of the gradient profile are biocompatible and allow useful applications in cell biological research.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: National Institutes of Health (NIH); National Science Foundation (NSF); USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1816134

Journal Information:: Biomedical Microdevices, Journal Name: Biomedical Microdevices Journal Issue: 5 Vol. 11; ISSN 1387-2176

Publisher:: Springer NatureCopyright Statement

Country of Publication:: United States

Language:: English

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Rapid Fabrication of Complex 3D Extracellular Microenvironments by Dynamic Optical Projection Stereolithography Zhang, A. Ping; Qu, Xin; Soman, Pranav Advanced Materials, Vol. 24, Issue 31 https://doi.org/10.1002/adma.201202024	journal	July 2012
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36 MATERIALS SCIENCE
59 BASIC BIOLOGICAL SCIENCES
DMD (digital micromirror device)
Non-canonical amino acids
Protein engineering
Protein gradient
Protein lithography
Protein patterning

Gradient lithography of engineered proteins to fabricate 2D and 3D cell culture microenvironments

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References (37)

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