Mutations in Arabidopsis YCF20-like genes affect thermal dissipation of excess absorbed light energy

Jung, Hou-Sung; Niyogi, Krishna K.

doi:10.1007/s00425-010-1098-9

Mutations in Arabidopsis YCF20-like genes affect thermal dissipation of excess absorbed light energy

Journal Article · Wed Jan 20 00:00:00 EST 2010 · Planta

DOI:https://doi.org/10.1007/s00425-010-1098-9· OSTI ID:1815720

Jung, Hou-Sung ^[1]; Niyogi, Krishna K. ^[2]

Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology; OSTI
Univ. of California, Berkeley, CA (United States). Dept. of Plant and Microbial Biology

Plants dissipate excess absorbed light energy as heat to protect themselves from photo-oxidative stress. The Arabidopsis thaliana npq6 mutant affected in thermal dissipation was identified by its partial defect in the induction of nonphotochemical quenching of chlorophyll fluorescence (NPQ) by excess light. Positional cloning revealed that npq6 contains a frameshift mutation caused by a single base-pair deletion in the At5g43050 gene, which encodes a member of the hypothetical chloroplast open reading frame 20 (YCF20) family of proteins with unknown function(s). The YCF20 protein family is mostly conserved in oxygenic photosynthetic organisms including cyanobacteria, eukaryotic algae, and plants. Amino acid sequence comparison identified two other genes in Arabidopsis that encode similar proteins to NPQ6: At1g65420 and At3g56830. These three Arabidopsis proteins have functional chloroplast-targeting transit peptides. Using reverse genetics, a mutant with a T-DNA insertion within the At1g65420 gene was identified and shown to exhibit a low NPQ phenotype similar to that of npq6; therefore, At1g65420 was named NPQ7. In contrast, a knockdown mutant in the At3g56830 gene with lower transcript levels showed wild-type levels of NPQ. The npq6 npq7 double mutant had an additive NPQ defect, indicating that the YCF20 family members in Arabidopsis have overlapping functions affecting thermal dissipation.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES). Chemical Sciences, Geosciences & Biosciences Division

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1815720

Journal Information:: Planta, Journal Name: Planta Journal Issue: 4 Vol. 231; ISSN 0032-0935

Publisher:: SpringerCopyright Statement

Country of Publication:: United States

Language:: English

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