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Arabidopsis thaliana PGR7 Encodes a Conserved Chloroplast Protein That Is Necessary for Efficient Photosynthetic Electron Transport

Journal Article · · PLoS ONE
 [1];  [2];  [3];  [4];  [5];  [5];  [3];  [6];  [7]
  1. University of California, Berkeley, CA (United States); DOE/OSTI
  2. Kyushu University, Fukuoka (Japan); Kyoto University (Japan)
  3. University of California, Berkeley, CA (United States)
  4. University of California, Berkeley, CA (United States). Dept. of Bioengineering
  5. Colorado State University, Fort Collins, CO (United States)
  6. Kyoto University (Japan)
  7. University of California, Berkeley, CA (United States); Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
+ Show Author Affiliations
A significant fraction of a plant’s nuclear genome encodes chloroplast-targeted proteins, many of which are devoted to the assembly and function of the photosynthetic apparatus. Using digital video imaging of chlorophyll fluorescence, we isolated proton gradient regulation 7 (pgr7) as an Arabidopsis thaliana mutant with low nonphotochemical quenching of chlorophyll fluorescence (NPQ). In pgr7, the xanthophyll cycle and the PSBS gene product, previously identified NPQ factors, were still functional, but the efficiency of photosynthetic electron transport was lower than in the wild type. The pgr7 mutant was also smaller in size and had lower chlorophyll content than the wild type in optimal growth conditions. Positional cloning located the pgr7 mutation in the At3g21200 (PGR7) gene, which was predicted to encode a chloroplast protein of unknown function. Chloroplast targeting of PGR7 was confirmed by transient expression of a GFP fusion protein and by stable expression and subcellular localization of an epitope-tagged version of PGR7. Bioinformatic analyses revealed that the PGR7 protein has two domains that are conserved in plants, algae, and bacteria, and the N-terminal domain is predicted to bind a cofactor such as FMN. Thus, we identified PGR7 as a novel, conserved nuclear gene that is necessary for efficient photosynthetic electron transport in chloroplasts of Arabidopsis.
Research Organization:
Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
Sponsoring Organization:
Japan Society for the Promotion of Science (JSPS); National Science Foundation (NSF); US Department of Agriculture; USDOE Office of Science (SC), Biological and Environmental Research (BER)
Grant/Contract Number:
AC02-05CH11231
OSTI ID:
1627416
Journal Information:
PLoS ONE, Journal Name: PLoS ONE Journal Issue: 7 Vol. 5; ISSN 1932-6203
Publisher:
Public Library of ScienceCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (4)

The Arabidopsis glutamyl-tRNA reductase (GluTR) forms a ternary complex with FLU and GluTR-binding protein journal January 2016
Growth and development of Arabidopsis thaliana under single-wavelength red and blue laser light journal September 2016
Thylakoid redox signals are integrated into organellar-gene-expression-dependent retrograde signaling in the prors1-1 mutant journal January 2012
Functional characterization of the two ferrochelatases in Arabidopsis thaliana : Distinct functions of journal January 2014

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Related Subjects

54 ENVIRONMENTAL SCIENCES
59 BASIC BIOLOGICAL SCIENCES
Arabidopsis thaliana
chlorophyll
chloroplasts
fluorescence
fluorescence imaging
light
photosynthesis
phylogenetic analysis