Spatial Mapping of Pyocyanin in Pseudomonas Aeruginosa Bacterial Communities Using Surface Enhanced Raman Scattering

Polisetti, Sneha; Baig, Nameera F.; Morales-Soto, Nydia; Shrout, Joshua D.; Bohn, Paul W.

doi:10.1177/0003702816654167

Spatial Mapping of Pyocyanin in Pseudomonas Aeruginosa Bacterial Communities Using Surface Enhanced Raman Scattering

Journal Article · Tue Jun 28 04:00:00 EDT 2016 · Applied Spectroscopy

DOI:https://doi.org/10.1177/0003702816654167· OSTI ID:1811273

Polisetti, Sneha ^[1]; Baig, Nameera F. ^[2]; Morales-Soto, Nydia ^[2]; Shrout, Joshua D. ^[2]; Bohn, Paul W. ^[2]

Univ. of Notre Dame, IN (United States); University of Notre Dame
Univ. of Notre Dame, IN (United States)

Surface enhanced Raman spectroscopy (SERS) imaging was used in conjunction with principal component analysis (PCA) for the in situ spatiotemporal mapping of the virulence factor pyocyanin in communities of the pathogenic bacterium Pseudomonas aeruginosa. The combination of SERS imaging and PCA analysis provides a robust method for the characterization of heterogeneous biological systems while circumventing issues associated with interference from sample autofluorescence and low reproducibility of SERS signals. The production of pyocyanin is found to depend both on the growth carbon source and on the specific strain of P. aeruginosa studied. A cystic fibrosis lung isolate strain of P. aeruginosa synthesizes and secretes pyocyanin when grown with glucose and glutamate, while the laboratory strain exhibits detectable production of pyocyanin only when grown with glutamate as the source of carbon. Pyocyanin production in the laboratory strain grown with glucose was below the limit of detection of SERS. In addition, the combination of SERS imaging and PCA can elucidate subtle differences in the molecular composition of biofilms. As a result, PCA loading plots from the clinical isolate exhibit features corresponding to vibrational bands of carbohydrates, which represent the mucoid biofilm matrix specific to that isolate, features that are not seen in the PCA loading plots of the laboratory strain.

View Accepted Manuscript (DOE)

Research Organization:: Univ. of Notre Dame, IN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1811273

Journal Information:: Applied Spectroscopy, Journal Name: Applied Spectroscopy Journal Issue: 2 Vol. 71; ISSN 0003-7028

Publisher:: Society for Applied SpectroscopyCopyright Statement

Country of Publication:: United States

Language:: English

References (45)

Surface-enhanced Raman spectroscopy: a brief retrospective Moskovits, Martin Journal of Raman Spectroscopy, Vol. 36, Issue 6-7 https://doi.org/10.1002/jrs.1362	journal	January 2005
Towards chemical analysis of nanostructures in biofilms II: tip-enhanced Raman spectroscopy of alginates Schmid, Thomas; Messmer, Andreas; Yeo, Boon-Siang Analytical and Bioanalytical Chemistry, Vol. 391, Issue 5 https://doi.org/10.1007/s00216-008-2101-1	journal	April 2008
Layer-by-layer coating of bacteria with noble metal nanoparticles for surface-enhanced Raman scattering Kahraman, Mehmet; Zamaleeva, Alsu I.; Fakhrullin, Rawil F. Analytical and Bioanalytical Chemistry, Vol. 395, Issue 8 https://doi.org/10.1007/s00216-009-3159-0	journal	October 2009
Metabolism and function of phenazines in bacteria: impacts on the behavior of bacteria in the environment and biotechnological processes Pierson, Leland S.; Pierson, Elizabeth A. Applied Microbiology and Biotechnology, Vol. 86, Issue 6 https://doi.org/10.1007/s00253-010-2509-3	journal	March 2010
[3] Quorum sensing signals in development of Pseudomonas aeruginosa biofilms Parsek, Matthew R.; Peter Greenberg, E. Biofilms https://doi.org/10.1016/S0076-6879(99)10005-3	book	January 1999
Single-cell analysis of bacteria by Raman microscopy: spectral information on the chemical composition of cells and on the heterogeneity in a culture Schuster, K. C.; Urlaub, E.; Gapes, J. R. Journal of Microbiological Methods, Vol. 42, Issue 1 https://doi.org/10.1016/S0167-7012(00)00169-X	journal	September 2000
An overview of the pathogenesis of cystic fibrosis lung disease Boucher, R. C. Advanced Drug Delivery Reviews, Vol. 54, Issue 11 https://doi.org/10.1016/S0169-409X(02)00144-8	journal	December 2002
Novel nanostructures for SERS biosensing Tripp, Ralph A.; Dluhy, Richard A.; Zhao, Yiping Nano Today, Vol. 3, Issue 3-4 https://doi.org/10.1016/S1748-0132(08)70042-2	journal	June 2008
The role of pyocyanin in Pseudomonas aeruginosa infection Lau, Gee W.; Hassett, Daniel J.; Ran, Huimin Trends in Molecular Medicine, Vol. 10, Issue 12 https://doi.org/10.1016/j.molmed.2004.10.002	journal	December 2004
Culture-free diagnostics of Pseudomonas aeruginosa infection by silver nanorod array based SERS from clinical sputum samples Wu, Xiaomeng; Chen, Jing; Li, Xibo Nanomedicine: Nanotechnology, Biology and Medicine, Vol. 10, Issue 8 https://doi.org/10.1016/j.nano.2014.04.010	journal	November 2014
Discrimination of Bacteria Using Surface-Enhanced Raman Spectroscopy Jarvis, Roger M.; Goodacre, Royston Analytical Chemistry, Vol. 76, Issue 1 https://doi.org/10.1021/ac034689c	journal	January 2004
SERS Detection of Bacteria in Water by in Situ Coating with Ag Nanoparticles Zhou, Haibo; Yang, Danting; Ivleva, Natalia P. Analytical Chemistry, Vol. 86, Issue 3 https://doi.org/10.1021/ac402935p	journal	January 2014
Raman Spectroscopic Method for Identification of Clinically Relevant Microorganisms Growing on Solid Culture Medium Maquelin, Kees; Choo-Smith, Lin-P'ing; van Vreeswijk, Tamara Analytical Chemistry, Vol. 72, Issue 1 https://doi.org/10.1021/ac991011h	journal	January 2000
A Unified View of Surface-Enhanced Raman Scattering Lombardi, John R.; Birke, Ronald L. Accounts of Chemical Research, Vol. 42, Issue 6 https://doi.org/10.1021/ar800249y	journal	June 2009
Surface-Enhanced Raman Spectroscopy (SERS) for Environmental Analyses Halvorson, Rebecca A.; Vikesland, Peter J. Environmental Science & Technology, Vol. 44, Issue 20 https://doi.org/10.1021/es101228z	journal	October 2010
Adsorption and surface-enhanced Raman of dyes on silver and gold sols Lee, P. C.; Meisel, D. The Journal of Physical Chemistry, Vol. 86, Issue 17, p. 3391-3395 https://doi.org/10.1021/j100214a025	journal	August 1982
Intrinsic Fluorescence and Quenching Effects in Photoactivated Reactively Sputtered Silver Oxide Layers Mihalcea, Christophe; Büchel, Dorothea; Atoda, Nobufumi Journal of the American Chemical Society, Vol. 123, Issue 29 https://doi.org/10.1021/ja015932x	journal	July 2001
Structure−Activity Relationships in Gold Nanoparticle Dimers and Trimers for Surface-Enhanced Raman Spectroscopy Wustholz, Kristin L.; Henry, Anne-Isabelle; McMahon, Jeffrey M. Journal of the American Chemical Society, Vol. 132, Issue 31 https://doi.org/10.1021/ja104174m	journal	July 2010
Probing the Structure of Single-Molecule Surface-Enhanced Raman Scattering Hot Spots Camden, Jon P.; Dieringer, Jon A.; Wang, Yingmin Journal of the American Chemical Society, Vol. 130, Issue 38, p. 12616-12617 https://doi.org/10.1021/ja8051427	journal	September 2008
Label-Free Surface-Enhanced Raman Spectroscopy Detection of DNA with Single-Base Sensitivity Xu, Li-Jia; Lei, Zhi-Chao; Li, Jiuxing Journal of the American Chemical Society, Vol. 137, Issue 15 https://doi.org/10.1021/jacs.5b01426	journal	April 2015
Photochemical Reactions of Phenazine and Acridine Adsorbed on Silver Colloid Surfaces Jeong, Dae Hong; Suh, Jung Sang; Moskovits, Martin The Journal of Physical Chemistry B, Vol. 104, Issue 31 https://doi.org/10.1021/jp001730w	journal	August 2000
Proof of Single-Molecule Sensitivity in Surface Enhanced Raman Scattering (SERS) by Means of a Two-Analyte Technique Le Ru, E. C.; Meyer, M.; Etchegoin, P. G. The Journal of Physical Chemistry B, Vol. 110, Issue 4, p. 1944-1948 https://doi.org/10.1021/jp054732v	journal	February 2006
Quantifying Resonant Raman Cross Sections with SERS Meyer, Stefan A.; Ru, Eric C. Le; Etchegoin, Pablo G. The Journal of Physical Chemistry A, Vol. 114, Issue 17 https://doi.org/10.1021/jp100669q	journal	May 2010
Determination of the Distance Dependence and Experimental Effects for Modified SERS Substrates Based on Self-Assembled Monolayers Formed Using Alkanethiols Kennedy, B. J.; Spaeth, S.; Dickey, M. The Journal of Physical Chemistry B, Vol. 103, Issue 18 https://doi.org/10.1021/jp984454i	journal	May 1999
Erratum: Corrigendum: Rethinking 'secondary' metabolism: physiological roles for phenazine antibiotics Price-Whelan, Alexa; Dietrich, Lars E. P.; Newman, Dianne K. Nature Chemical Biology, Vol. 2, Issue 4 https://doi.org/10.1038/nchembio0406-221a	journal	April 2006
Up-regulating pyocyanin production by amino acid addition for early electrochemical identification of Pseudomonas aeruginosa Sismaet, Hunter J.; Webster, Thaddaeus A.; Goluch, Edgar D. The Analyst, Vol. 139, Issue 17 https://doi.org/10.1039/C4AN00756E	journal	January 2014
Correlated mass spectrometry imaging and confocal Raman microscopy for studies of three-dimensional cell culture sections Ahlf, Dorothy R.; Masyuko, Rachel N.; Hummon, Amanda B. The Analyst, Vol. 139, Issue 18 https://doi.org/10.1039/C4AN00826J	journal	January 2014
Characterisation and identification of bacteria using SERS Jarvis, Roger M.; Goodacre, Royston Chemical Society Reviews, Vol. 37, Issue 5 https://doi.org/10.1039/b705973f	journal	January 2008
SERS as a bioassay platform: fundamentals, design, and applications Porter, Marc D.; Lipert, Robert J.; Siperko, Lorraine M. Chemical Society Reviews, Vol. 37, Issue 5 https://doi.org/10.1039/b708461g	journal	January 2008
Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis O'Toole, George A.; Kolter, Roberto Molecular Microbiology, Vol. 28, Issue 3 https://doi.org/10.1046/j.1365-2958.1998.00797.x	journal	April 1998
Surface–enhanced Raman scattering on single–wall carbon nanotubes Kneipp, Katrin; Kneipp, Harald; Dresselhaus, Mildred S. Philosophical Transactions of the Royal Society of London. Series A: Mathematical, Physical and Engineering Sciences, Vol. 362, Issue 1824 https://doi.org/10.1098/rsta.2004.1445	journal	September 2004
The impact of quorum sensing and swarming motility on Pseudomonas aeruginosa biofilm formation is nutritionally conditional Shrout, Joshua D.; Chopp, David L.; Just, Collin L. Molecular Microbiology, Vol. 62, Issue 5 https://doi.org/10.1111/j.1365-2958.2006.05421.x	journal	December 2006
Phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by Pythium spp. Perneel, Maaike; D'hondt, Liesbet; De Maeyer, Katrien Environmental Microbiology, Vol. 10, Issue 3 https://doi.org/10.1111/j.1462-2920.2007.01501.x	journal	March 2008
Quorum sensing in Pseudomonas aeruginosa biofilms de Kievit, T. R. Environmental Microbiology, Vol. 11, Issue 2 https://doi.org/10.1111/j.1462-2920.2008.01792.x	journal	February 2009
Bacterial and Clinical Characteristics of Health Care- and Community-Acquired Bloodstream Infections Due to Pseudomonas aeruginosa Hattemer, Angela; Hauser, Alan; Diaz, Maureen Antimicrobial Agents and Chemotherapy, Vol. 57, Issue 8 https://doi.org/10.1128/AAC.02467-12	journal	June 2013
Catabolite Repression Control of Pyocyanin Biosynthesis at an Intersection of Primary and Secondary Metabolism in Pseudomonas aeruginosa Huang, Jiaofang; Sonnleitner, Elisabeth; Ren, Bin Applied and Environmental Microbiology, Vol. 78, Issue 14 https://doi.org/10.1128/AEM.00026-12	journal	July 2012
Genetic mapping of chromosomal determinants for the production of the exopolysaccharide alginate in a Pseudomonas aeruginosa cystic fibrosis isolate Ohman, D. E.; Chakrabarty, A. M. Infection and Immunity, Vol. 33, Issue 1 https://doi.org/10.1128/IAI.33.1.142-148.1981	journal	July 1981
Nutritional Cues Control Pseudomonas aeruginosa Multicellular Behavior in Cystic Fibrosis Sputum Palmer, Kelli L.; Aye, Lindsay M.; Whiteley, Marvin Journal of Bacteriology, Vol. 191, Issue 8 https://doi.org/10.1128/JB.00185-09	journal	April 2009
The EPS Matrix: The "House of Biofilm Cells" Flemming, H. -C.; Neu, T. R.; Wozniak, D. J. Journal of Bacteriology, Vol. 189, Issue 22 https://doi.org/10.1128/JB.00858-07	journal	August 2007
Timing and Localization of Rhamnolipid Synthesis Gene Expression in Pseudomonas aeruginosa Biofilms Lequette, Yannick; Greenberg, E. P. Journal of Bacteriology, Vol. 187, Issue 1 https://doi.org/10.1128/JB.187.1.37-44.2005	journal	January 2005
Mucoid Pseudomonas in Cystic Fibrosis Pritt, Bobbi; O’Brien, Linda; Winn, Washington American Journal of Clinical Pathology, Vol. 128, Issue 1 https://doi.org/10.1309/KJRPC7DD5TR9NTDM	journal	July 2007
Part I: Surface-Enhanced Raman Spectroscopy Investigation of Amino Acids and Their Homodipeptides Adsorbed on Colloidal Silver Podstawka, Edyta; Ozaki, Yukihiro; Proniewicz, Leonard M. Applied Spectroscopy, Vol. 58, Issue 5 https://doi.org/10.1366/000370204774103408	journal	May 2004
Rapid and Sensitive Detection of Rotavirus Molecular Signatures Using Surface Enhanced Raman Spectroscopy Driskell, Jeremy D.; Zhu, Yu; Kirkwood, Carl D. PLoS ONE, Vol. 5, Issue 4 https://doi.org/10.1371/journal.pone.0010222	journal	April 2010
The Pseudomonas aeruginosa Reference Strain PA14 Displays Increased Virulence Due to a Mutation in ladS Mikkelsen, Helga; McMullan, Rachel; Filloux, Alain PLoS ONE, Vol. 6, Issue 12 https://doi.org/10.1371/journal.pone.0029113	journal	December 2011
Pseudomonas aeruginosa Exotoxin Pyocyanin Causes Cystic Fibrosis Airway Pathogenesis Caldwell, Charles C.; Chen, Yi; Goetzmann, Holly S. The American Journal of Pathology, Vol. 175, Issue 6 https://doi.org/10.2353/ajpath.2009.090166	journal	December 2009

Cited By (5)

A multifunctional SERS sticky note for real-time quorum sensing tracing and inactivation of bacterial biofilms Guo, Jingxing; Liu, Ying; Chen, Yunlong Chemical Science, Vol. 9, Issue 27 https://doi.org/10.1039/c8sc02078g	journal	January 2018
Review on SERS of Bacteria Mosier-Boss, Pamela Biosensors, Vol. 7, Issue 4 https://doi.org/10.3390/bios7040051	journal	November 2017
Detection of Pseudomonas aeruginosa Metabolite Pyocyanin in Water and Saliva by Employing the SERS Technique Žukovskaja, Olga; Jahn, Izabella; Weber, Karina Sensors, Vol. 17, Issue 8 https://doi.org/10.3390/s17081704	journal	July 2017
Chemosensory epithelial cells in the urethra: sentinels of the urinary tract Deckmann, Klaus; Kummer, Wolfgang Histochemistry and Cell Biology, Vol. 146, Issue 6 https://doi.org/10.1007/s00418-016-1504-x	journal	September 2016
Surface-Enhanced Raman Scattering (SERS) in Microbiology: Illumination and Enhancement of the Microbial World Chisanga, Malama; Muhamadali, Howbeer; Ellis, David I. Applied Spectroscopy, Vol. 72, Issue 7 https://doi.org/10.1177/0003702818764672	journal	March 2018

Similar Records

Label-free molecular imaging of bacterial communities of the opportunistic pathogen Pseudomonas aeruginosa

Journal Article · Tue Sep 27 00:00:00 EDT 2016 · Proceedings of SPIE - The International Society for Optical Engineering · OSTI ID:1807684

Electrochemical Surface-Enhanced Raman Spectroscopy of Pyocyanin Secreted by Pseudomonas aeruginosa Communities

Journal Article · Wed May 01 00:00:00 EDT 2019 · Langmuir · OSTI ID:1765171

Draft Genome Sequence of a Stable Mucoid Strain of Pseudomonas aeruginosa PAO581 with a mucA25 Mutation

Journal Article · Thu Aug 29 00:00:00 EDT 2013 · Genome Announcements · OSTI ID:1629281

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PCA
Pseudomonas aeruginosa
SERS
biofilms
principal component analysis
pyocyanin
surface enhanced Raman spectroscopy

Spatial Mapping of Pyocyanin in Pseudomonas Aeruginosa Bacterial Communities Using Surface Enhanced Raman Scattering

Citation Formats

References (45)

Cited By (5)

Similar Records

Related Subjects