Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440

Werner, Allison Z.; Clare, Rita; Mand, Thomas D.; Pardo, Isabel; Ramirez, Kelsey J.; Haugen, Stefan J.; Bratti, Felicia; Dexter, Gara N.; Elmore, Joshua R.; Huenemann, Jay D.; Peabody, V, George L.; Johnson, Christopher W.; Rorrer, Nicholas A.; Salvachúa, Davinia; Guss, Adam M.; Beckham, Gregg T.

doi:10.1016/j.ymben.2021.07.005

Title: Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440

Journal Article · Wed Sep 01 00:00:00 EDT 2021 · Metabolic Engineering

DOI:https://doi.org/10.1016/j.ymben.2021.07.005· OSTI ID:1807635

;

; Mand, Thomas D.;

; Ramirez, Kelsey J.;

; Bratti, Felicia; Dexter, Gara N.;

; Huenemann, Jay D.; Peabody, V, George L.; Johnson, Christopher W.;

; Salvachúa, Davinia;

;

Poly(ethylene terephthalate) (PET) is the most abundantly consumed synthetic polyester and accordingly a major source of plastic waste. The development of chemocatalytic approaches for PET depolymerization to monomers offers new options for open-loop upcycling of PET, which can leverage biological transformations to higher-value products. To that end, here we perform four sequential metabolic engineering efforts in Pseudomonas putida KT2440 to enable the conversion of PET glycolysis products via: (i) ethylene glycol utilization by constitutive expression of native genes, (ii) terephthalate (TPA) catabolism by expression of tphA2_IIA3_IIB_IIA1_II from Comamonas and tpaK from Rhodococcus jostii, (iii) bis(2-hydroxyethyl) terephthalate (BHET) hydrolysis to TPA by expression of PETase and MHETase from Ideonella sakaiensis, and (iv) BHET conversion to a performance-advantaged bioproduct, ß-ketoadipic acid (ßKA) by deletion of pcaIJ. Using this strain, we demonstrate production of 15.1 g/L ßKA from BHET at 76% molar yield in bioreactors and conversion of catalytically depolymerized PET to ßKA. Overall, this work highlights the potential of tandem catalytic deconstruction and biological conversion as a means to upcycle waste PET.

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Research Organization:: National Renewable Energy Lab. (NREL), Golden, CO (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Energy Efficiency Office. Advanced Manufacturing Office; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office

Grant/Contract Number:: AC05-00OR22725; AC36-08GO28308

OSTI ID:: 1807635

Alternate ID(s):: OSTI ID: 1814600; OSTI ID: 1818959

Report Number(s):: NREL/JA-2800-80145; S1096717621001154; PII: S1096717621001154

Journal Information:: Metabolic Engineering, Journal Name: Metabolic Engineering Vol. 67 Journal Issue: C; ISSN 1096-7176

Publisher:: ElsevierCopyright Statement

Country of Publication:: Belgium

Language:: English

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Title: Tandem chemical deconstruction and biological upcycling of poly(ethylene terephthalate) to β-ketoadipic acid by Pseudomonas putida KT2440

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