Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source

Rubinstein, Gabriel M.; Lipscomb, Gina L.; Williams-Rhaesa, Amanda M.; Schut, Gerrit J.; Kelly, Robert M.; Adams, Michael W. W.

doi:10.1007/s10295-020-02299-z

Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source

Journal Article · 01 August 2020 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-020-02299-z· OSTI ID:1803773

Rubinstein, Gabriel M. ^[1]; Lipscomb, Gina L. ^[2]; Williams-Rhaesa, Amanda M. ^[2]; Schut, Gerrit J. ^[2]; Kelly, Robert M. ^[3]; Adams, Michael W. W. ^[2]

grid.213876.9 0000 0004 1936 738X Department of Biochemistry and Molecular Biology University of Georgia 30602 Athens GA USA; OSTI
grid.213876.9 0000 0004 1936 738X Department of Biochemistry and Molecular Biology University of Georgia 30602 Athens GA USA
grid.40803.3f 0000 0001 2173 6074 Department of Chemical and Biomolecular Engineering North Carolina State University 27695 Raleigh NC USA

Abstract

Caldicellulosiruptor bescii is the most thermophilic cellulolytic organism yet identified (Topt 78 °C). It grows on untreated plant biomass and has an established genetic system thereby making it a promising microbial platform for lignocellulose conversion to bio-products. Here, we investigated the ability of engineered C. bescii to generate alcohols from carboxylic acids. Expression of aldehyde ferredoxin oxidoreductase (aor from Pyrococcus furiosus) and alcohol dehydrogenase (adhA from Thermoanaerobacter sp. X514) enabled C. bescii to generate ethanol from crystalline cellulose and from biomass by reducing the acetate produced by fermentation. Deletion of lactate dehydrogenase in a strain expressing the AOR–Adh pathway increased ethanol production. Engineered strains also converted exogenously supplied organic acids (isobutyrate and n-caproate) to the corresponding alcohol (isobutanol and hexanol) using both crystalline cellulose and switchgrass as sources of reductant for alcohol production. This is the first instance of an acid to alcohol conversion pathway in a cellulolytic microbe.

Research Organization:: Univ. of Georgia, Athens, GA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: SC0019391;

OSTI ID:: 1803773

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Journal Issue: 8 Vol. 47; ISSN 1367-5435

Publisher:: Springer

Country of Publication:: United States

Language:: English

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