Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source

Rubinstein, Gabriel M.; Lipscomb, Gina L.; Williams-Rhaesa, Amanda M.; Schut, Gerrit J.; Kelly, Robert M.; Adams, Michael W. W.

doi:10.1007/s10295-020-02299-z

Title: Engineering the cellulolytic extreme thermophile Caldicellulosiruptor bescii to reduce carboxylic acids to alcohols using plant biomass as the energy source

Journal Article · Sat Aug 01 00:00:00 EDT 2020 · Journal of Industrial Microbiology and Biotechnology

DOI:https://doi.org/10.1007/s10295-020-02299-z· OSTI ID:1772496

Rubinstein, Gabriel M. ^[1]; Lipscomb, Gina L. ^[1]; Williams-Rhaesa, Amanda M. ^[1]; Schut, Gerrit J. ^[1]; Kelly, Robert M. ^[2]; Adams, Michael W. W. ^[1]

grid.213876.9 0000 0004 1936 738X Department of Biochemistry and Molecular Biology University of Georgia 30602 Athens GA USA
grid.40803.3f 0000 0001 2173 6074 Department of Chemical and Biomolecular Engineering North Carolina State University 27695 Raleigh NC USA

Abstract Caldicellulosiruptor bescii is the most thermophilic cellulolytic organism yet identified (Topt 78 °C). It grows on untreated plant biomass and has an established genetic system thereby making it a promising microbial platform for lignocellulose conversion to bio-products. Here, we investigated the ability of engineered C. bescii to generate alcohols from carboxylic acids. Expression of aldehyde ferredoxin oxidoreductase (aor from Pyrococcus furiosus) and alcohol dehydrogenase (adhA from Thermoanaerobacter sp. X514) enabled C. bescii to generate ethanol from crystalline cellulose and from biomass by reducing the acetate produced by fermentation. Deletion of lactate dehydrogenase in a strain expressing the AOR–Adh pathway increased ethanol production. Engineered strains also converted exogenously supplied organic acids (isobutyrate and n-caproate) to the corresponding alcohol (isobutanol and hexanol) using both crystalline cellulose and switchgrass as sources of reductant for alcohol production. This is the first instance of an acid to alcohol conversion pathway in a cellulolytic microbe.

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Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: SC0019391; PS02-06ER64304

OSTI ID:: 1772496

Journal Information:: Journal of Industrial Microbiology and Biotechnology, Journal Name: Journal of Industrial Microbiology and Biotechnology Vol. 47 Journal Issue: 8; ISSN 1367-5435

Publisher:: Oxford University PressCopyright Statement

Country of Publication:: Germany

Language:: English

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