Photosynthetic Co-production of Succinate and Ethylene in a Fast-Growing Cyanobacterium, Synechococcus elongatus PCC 11801

Sengupta, Annesha; Pritam, Prem; Jaiswal, Damini; Bandyopadhyay, Anindita; Pakrasi, Himadri B.; Wangikar, Pramod P.

doi:10.3390/metabo10060250

Photosynthetic Co-production of Succinate and Ethylene in a Fast-Growing Cyanobacterium, Synechococcus elongatus PCC 11801

Journal Article · Tue Jun 16 00:00:00 EDT 2020 · Metabolites

DOI:https://doi.org/10.3390/metabo10060250· OSTI ID:1803771

^[1]; Pritam, Prem ^[2]; ^[2]; Bandyopadhyay, Anindita ^[3]; ^[4]; ^[5]

Indian Inst. of Technology (IIT) Bombay, Mumbai (India). Dept. of Chemical Engineering; OSTI
Indian Inst. of Technology (IIT) Bombay, Mumbai (India). Dept. of Chemical Engineering
Washington Univ., St. Louis, MO (United States). Dept. of Biology
Washington Univ., St. Louis, MO (United States). Dept. of Biology; Washington Univ., St. Louis, MO (United States). Dept. of Energy, Environmental and Chemical Engineering
Indian Inst. of Technology (IIT) Bombay, Mumbai (India). Dept. of Chemical Engineering; Indian Inst. of Technology (IIT) Bombay, Mumbai (India). DBT-Pan IIT Center for Bioenergy; Indian Inst. of Technology (IIT) Bombay, Mumbai (India). Wadhwani Research Center for Bioengineering

Cyanobacteria are emerging as hosts for photoautotrophic production of chemicals. Recent studies have attempted to stretch the limits of photosynthetic production, typically focusing on one product at a time, possibly to minimise the additional burden of product separation. Here, we explore the simultaneous production of two products that can be easily separated: ethylene, a gaseous product, and succinate, an organic acid that accumulates in the culture medium. This was achieved by expressing a single copy of the ethylene forming enzyme (efe) under the control of P_cpcB, the inducer-free super-strong promoter of phycocyanin β subunit. We chose the recently reported, fast-growing and robust cyanobacterium, Synechococcus elongatus PCC 11801, as the host strain. A stable recombinant strain was constructed using CRISPR-Cpf1 in a first report of markerless genome editing of this cyanobacterium. Under photoautotrophic conditions, the recombinant strain shows specific productivities of 338.26 and 1044.18 μmole/g dry cell weight/h for ethylene and succinate, respectively. These results compare favourably with the reported productivities for individual products in cyanobacteria that are highly engineered. Metabolome profiling and ¹³C labelling studies indicate carbon flux redistribution and suggest avenues for further improvement. Our results show that S. elongatus PCC 11801 is a promising candidate for metabolic engineering.

View Accepted Manuscript (DOE)

Research Organization:: Washington Univ., St. Louis, MO (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: SC0019386

OSTI ID:: 1803771

Journal Information:: Metabolites, Journal Name: Metabolites Journal Issue: 6 Vol. 10; ISSN 2218-1989; ISSN METALU

Publisher:: MDPICopyright Statement

Country of Publication:: United States

Language:: English

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Increased ethylene production by overexpressing phosphoenolpyruvate carboxylase in the cyanobacterium Synechocystis PCC 6803 Durall, Claudia; Lindberg, Pia; Yu, Jianping figshare https://doi.org/10.6084/m9.figshare.c.4834587.v2	collection	January 2020

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