Two bacterial strains, 1NTand 5NT, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1NT(0.65×1.85 µm) and 5NT(0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5NTelongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1NTand 5NToccurred at 25–30 °C, pH 6.5–7.0 and <0.5% salinity. Results of comparative chemotaxonomic, genomic and phylogenetic analyses revealed that 1NTand 5NTwere distinct from one another and their closest related type strains:Paraburkholderia madsenianaRP11T,Paraburkholderia aspalathiLMG 27731TandParaburkholderia caffeinilyticaCF1T. The genomes of 1NTand 5NThad an average nucleotide identity (91.6 and 91.3%) andin silicoDNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1NTand 5NTfrom each other and their closest relatives. The only ubiquinone present in strains 1NTand 5NTwas Q-8. The major cellular fatty acids were C16 : 0, 3OH-C16 : 0, C17 : 0cyclo, C19 : 0cyclo ω8cand summed features 2 (3OH-C14 : 0/ C16 : 1iso I), 3 (C16 : 1ω6c/ω7c) and 8 (C18 : 1ω7c/ω6c). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain ofP. madseniana(ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description ofP. madseniana. Our polyphasic approach demonstrated that strains 1NTand 5NTrepresent novel species from the genusParaburkholderiafor which the namesParaburkholderia solitsugaesp. nov. (type strain 1NT=DSM 110721T=LMG 31704T) andParaburkholderia elongatasp. nov. (type strain 5NT=DSM 110722T=LMG 31705T) are proposed.
Wilhelm, Roland C., et al. "Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana." International Journal of Systematic and Evolutionary Microbiology, vol. 70, no. 9, Sep. 2020. https://doi.org/10.1099/ijsem.0.004387
Wilhelm, Roland C., Cyle, K. Taylor, Martinez, Carmen Enid, Karasz, David C., Newman, Jeffrey D., & Buckley, Daniel H. (2020). Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana. International Journal of Systematic and Evolutionary Microbiology, 70(9). https://doi.org/10.1099/ijsem.0.004387
Wilhelm, Roland C., Cyle, K. Taylor, Martinez, Carmen Enid, et al., "Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana," International Journal of Systematic and Evolutionary Microbiology 70, no. 9 (2020), https://doi.org/10.1099/ijsem.0.004387
@article{osti_1802954,
author = {Wilhelm, Roland C. and Cyle, K. Taylor and Martinez, Carmen Enid and Karasz, David C. and Newman, Jeffrey D. and Buckley, Daniel H.},
title = {Paraburkholderia solitsugae sp. nov. and Paraburkholderia elongata sp. nov., phenolic acid-degrading bacteria isolated from forest soil and emended description of Paraburkholderia madseniana},
annote = {Two bacterial strains, 1NTand 5NT, were isolated from hemlock forest soil using a soluble organic matter enrichment. Cells of 1NT(0.65×1.85 µm) and 5NT(0.6×1.85 µm) are Gram-stain-negative, aerobic, motile, non-sporulating and exist as single rods, diplobacilli or in chains of varying length. During growth in dilute media (≤0.1× tryptic soy broth; TSB), cells are primarily motile with flagella. At higher concentrations (≥0.3× TSB), cells of both strains increasingly form non-motile chains, and cells of 5NTelongate (0.57×~7 µm) and form especially long filaments. Optimum growth of 1NTand 5NToccurred at 25–30 °C, pH 6.5–7.0 and Tand 5NTwere distinct from one another and their closest related type strains:Paraburkholderia madsenianaRP11T,Paraburkholderia aspalathiLMG 27731TandParaburkholderia caffeinilyticaCF1T. The genomes of 1NTand 5NThad an average nucleotide identity (91.6 and 91.3%) andin silicoDNA–DNA hybridization values (45.8%±2.6 and 45.5%±2.5) and differed in functional gene content from their closest related type strains. The composition of fatty acids and patterns of substrate use, including the catabolism of phenolic acids, also differentiated strains 1NTand 5NTfrom each other and their closest relatives. The only ubiquinone present in strains 1NTand 5NTwas Q-8. The major cellular fatty acids were C16 : 0, 3OH-C16 : 0, C17 : 0cyclo, C19 : 0cyclo ω8cand summed features 2 (3OH-C14 : 0/ C16 : 1iso I), 3 (C16 : 1ω6c/ω7c) and 8 (C18 : 1ω7c/ω6c). A third bacterium, strain RL16-012-BIC-B, was isolated from soil associated with shallow roots and was determined to be a strain ofP. madseniana(ANI, 98.8%; 16S rRNA gene similarity, 100%). Characterizations of strain RL16-012-BIC-B (DSM 110723=LMG 31706) led to proposed emendments to the species description ofP. madseniana. Our polyphasic approach demonstrated that strains 1NTand 5NTrepresent novel species from the genusParaburkholderiafor which the namesParaburkholderia solitsugaesp. nov. (type strain 1NT=DSM 110721T=LMG 31704T) andParaburkholderia elongatasp. nov. (type strain 5NT=DSM 110722T=LMG 31705T) are proposed.},
doi = {10.1099/ijsem.0.004387},
url = {https://www.osti.gov/biblio/1802954},
journal = {International Journal of Systematic and Evolutionary Microbiology},
issn = {ISSN 1466-5026},
number = {9},
volume = {70},
place = {United States},
publisher = {International Union of Microbiological Societies},
year = {2020},
month = {09}}
International Journal of Systematic and Evolutionary Microbiology, Journal Name: International Journal of Systematic and Evolutionary Microbiology Journal Issue: 9 Vol. 70; ISSN 1466-5026