Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques

Fedick, Patrick W.; Morato, Nicolás M.; Pu, Fan; Cooks, R. Graham

doi:10.1016/j.ijms.2020.116326

Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques

Journal Article · Tue Mar 24 00:00:00 EDT 2020 · International Journal of Mass Spectrometry

DOI:https://doi.org/10.1016/j.ijms.2020.116326· OSTI ID:1800344

Fedick, Patrick W. ^[1]; Morato, Nicolás M. ^[2]; Pu, Fan ^[2]; Cooks, R. Graham ^[2]

Purdue Univ., West Lafayette, IN (United States); United States Navy Naval Air Systems Command (NAVAIR), China Lake, CA (United States); OSTI
Purdue Univ., West Lafayette, IN (United States)

We present a laboratory experiment, developed around a pair of instrumental analysis techniques, Raman spectroscopy and ambient ionization mass spectrometry (MS), conducted by senior chemistry undergraduate students for bulk analysis of over-the-counter drugs using benchtop versions of the two instruments, as well as trace analysis of illicit drugs utilizing the corresponding portable instruments. The identification and confirmation of seized drugs is a routine operation in forensic laboratories that can be simplified using these two instrumental techniques in tandem. Bulk samples are readily analyzed using conventional Raman spectroscopy followed by paper cone spray ionization (PCSI) MS, while trace analysis is allowed by a simple single-substrate dual-analyzer method using surface enhanced Raman spectroscopy (SERS) from paper followed by paper spray ionization (PSI) MS. The introduction of these novel strategies to the students exposed them to potential career paths in forensic science by means of a hands-on experience that provided fundamental knowledge about this set of analytical methods, including intercomparisons of their performance, as well as practical issues like costs, regulations and databases. The use of portable instrumentation represents an additional learning experience for the students, considering that it is cutting-edge technology that is just now being transferred from academic to industrial labs and that it allows a valuable discussion of the advantages and disadvantages of bringing the laboratory to the field.

View Accepted Manuscript (DOE)

Research Organization:: Purdue Univ., West Lafayette, IN (United States)

Sponsoring Organization:: USDOE; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: FG02-06ER15807

OSTI ID:: 1800344

Alternate ID(s):: OSTI ID: 1607942

Journal Information:: International Journal of Mass Spectrometry, Journal Name: International Journal of Mass Spectrometry Vol. 452; ISSN 1387-3806

Publisher:: ElsevierCopyright Statement

Country of Publication:: United States

Language:: English

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47 OTHER INSTRUMENTATION
Raman spectroscopy
ambient ionization
drugs of abuse
forensic analysis
hands-on learning
laboratory instruction
mass spectrometry
paper cone spray ionization
paper spray ionization
paper surface enhanced Raman spectroscopy

Raman spectroscopy coupled with ambient ionization mass spectrometry: A forensic laboratory investigation into rapid and simple dual instrumental analysis techniques

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