Designed Protein Cages as Scaffolds for Building Multienzyme Materials

McConnell, Scott A.; Cannon, Kevin A.; Morgan, Christian; McAllister, Rachel; Amer, Brendan R.; Clubb, Robert T.; Yeates, Todd O.

doi:10.1021/acssynbio.9b00407

Title: Designed Protein Cages as Scaffolds for Building Multienzyme Materials

Journal Article · Tue Dec 31 00:00:00 EST 2019 · ACS Synthetic Biology

DOI:https://doi.org/10.1021/acssynbio.9b00407· OSTI ID:1799610

McConnell, Scott A. ^[1]; Cannon, Kevin A. ^[2]; Morgan, Christian ^[3]; McAllister, Rachel ^[4]; Amer, Brendan R. ^[1];

^[1];

^[1]

UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, California 90095, United States; Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, United States; Molecular Biology Institute, University of California, Los Angeles, California 90095, United States
UCLA-DOE Institute for Genomics and Proteomics, Los Angeles, California 90095, United States; Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, United States
Department of Ecology and Evolutionary Biology, University of California, Los Angeles, California 90095, United States
Department of Chemistry and Biochemistry, University of California, Los Angeles, California 90095, United States

Not provided.

Cite

Export

Save

Research Organization:: Univ. of California, Los Angeles, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

DOE Contract Number:: FC02-02ER63421

OSTI ID:: 1799610

Journal Information:: ACS Synthetic Biology, Vol. 9, Issue 2; ISSN 2161-5063

Publisher:: American Chemical Society (ACS)

Country of Publication:: United States

Language:: English

References (93)

Nanohedra: Using symmetry to design self assembling protein cages, layers, crystals, and filaments Padilla, J. E.; Colovos, C.; Yeates, T. O. Proceedings of the National Academy of Sciences, Vol. 98, Issue 5 https://doi.org/10.1073/pnas.041614998	journal	February 2001
Computational Design of Self-Assembling Protein Nanomaterials with Atomic Level Accuracy King, N. P.; Sheffler, W.; Sawaya, M. R. Science, Vol. 336, Issue 6085 https://doi.org/10.1126/science.1219364	journal	May 2012
Structure of a 16-nm Cage Designed by Using Protein Oligomers Lai, Y. -T.; Cascio, D.; Yeates, T. O. Science, Vol. 336, Issue 6085 https://doi.org/10.1126/science.1219351	journal	May 2012
Accurate design of co-assembling multi-component protein nanomaterials King, Neil P.; Bale, Jacob B.; Sheffler, William Nature, Vol. 510, Issue 7503 https://doi.org/10.1038/nature13404	journal	May 2014
Accurate design of megadalton-scale two-component icosahedral protein complexes Bale, J. B.; Gonen, S.; Liu, Y. Science, Vol. 353, Issue 6297 https://doi.org/10.1126/science.aaf8818	journal	July 2016
Flexible, symmetry-directed approach to assembling protein cages Sciore, Aaron; Su, Min; Koldewey, Philipp Proceedings of the National Academy of Sciences, Vol. 113, Issue 31 https://doi.org/10.1073/pnas.1606013113	journal	July 2016
Symmetry-Directed Self-Assembly of a Tetrahedral Protein Cage Mediated by de Novo-Designed Coiled Coils Badieyan, Somayesadat; Sciore, Aaron; Eschweiler, Joseph D. ChemBioChem, Vol. 18, Issue 19 https://doi.org/10.1002/cbic.201700406	journal	August 2017
Coiled-Coil-Mediated Assembly of an Icosahedral Protein Cage with Extremely High Thermal and Chemical Stability Cristie-David, Ajitha S.; Chen, Junjie; Nowak, Derek B. Journal of the American Chemical Society, Vol. 141, Issue 23 https://doi.org/10.1021/jacs.8b13604	journal	May 2019
Natural and artificial protein cages: design, structure and therapeutic applications Heddle, Jonathan Gardiner; Chakraborti, Soumyananda; Iwasaki, Kenji Current Opinion in Structural Biology, Vol. 43 https://doi.org/10.1016/j.sbi.2017.03.007	journal	April 2017
Protein cage assembly across multiple length scales Aumiller, William M.; Uchida, Masaki; Douglas, Trevor Chemical Society Reviews, Vol. 47, Issue 10 https://doi.org/10.1039/C7CS00818J	journal	January 2018
High-symmetry protein assemblies: patterns and emerging applications Cannon, Kevin A.; Ochoa, Jessica M.; Yeates, Todd O. Current Opinion in Structural Biology, Vol. 55 https://doi.org/10.1016/j.sbi.2019.03.008	journal	April 2019
Evolution of a designed protein assembly encapsulating its own RNA genome Butterfield, Gabriel L.; Lajoie, Marc J.; Gustafson, Heather H. Nature, Vol. 552, Issue 7685 https://doi.org/10.1038/nature25157	journal	December 2017
Rational Engineering of a Designed Protein Cage for siRNA Delivery Edwardson, Thomas G. W.; Mori, Takahiro; Hilvert, Donald Journal of the American Chemical Society, Vol. 140, Issue 33 https://doi.org/10.1021/jacs.8b06442	journal	July 2018
Induction of Potent Neutralizing Antibody Responses by a Designed Protein Nanoparticle Vaccine for Respiratory Syncytial Virus Marcandalli, Jessica; Fiala, Brooke; Ols, Sebastian Cell, Vol. 176, Issue 6 https://doi.org/10.1016/j.cell.2019.01.046	journal	March 2019
Multivalent Display of Antifreeze Proteins by Fusion to Self-Assembling Protein Cages Enhances Ice-Binding Activities Phippen, Sean W.; Stevens, Corey A.; Vance, Tyler D. R. Biochemistry, Vol. 55, Issue 49 https://doi.org/10.1021/acs.biochem.6b00864	journal	November 2016
Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system Liu, Yuxi; Gonen, Shane; Gonen, Tamir Proceedings of the National Academy of Sciences, Vol. 115, Issue 13 https://doi.org/10.1073/pnas.1718825115	journal	March 2018
A 3.8 Å resolution cryo-EM structure of a small protein bound to an imaging scaffold Liu, Yuxi; Huynh, Duc T.; Yeates, Todd O. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-09836-0	journal	April 2019
Three-dimensional structure of the tryptophan synthase alpha 2 beta 2 multienzyme complex from Salmonella typhimurium. Hyde, C. C.; Ahmed, S. A.; Padlan, E. A. Journal of Biological Chemistry, Vol. 263, Issue 33 https://doi.org/10.1016/S0021-9258(19)77913-7	journal	November 1988
Bacterial microcompartments: widespread prokaryotic organelles for isolation and optimization of metabolic pathways: Bacterial microcompartments Bobik, Thomas A.; Lehman, Brent P.; Yeates, Todd O. Molecular Microbiology, Vol. 98, Issue 2 https://doi.org/10.1111/mmi.13117	journal	August 2015
Bacterial microcompartments Kerfeld, Cheryl A.; Aussignargues, Clement; Zarzycki, Jan Nature Reviews Microbiology, Vol. 16, Issue 5 https://doi.org/10.1038/nrmicro.2018.10	journal	March 2018
Using comparative genomics to uncover new kinds of protein-based metabolic organelles in bacteria: Bacterial Microcompartments Jorda, Julien; Lopez, David; Wheatley, Nicole M. Protein Science, Vol. 22, Issue 2 https://doi.org/10.1002/pro.2196	journal	January 2013
Origins of Activity Enhancement in Enzyme Cascades on Scaffolds Idan, Ofer; Hess, Henry ACS Nano, Vol. 7, Issue 10 https://doi.org/10.1021/nn402823k	journal	September 2013
Enzyme clustering accelerates processing of intermediates through metabolic channeling Castellana, Michele; Wilson, Maxwell Z.; Xu, Yifan Nature Biotechnology, Vol. 32, Issue 10 https://doi.org/10.1038/nbt.3018	journal	September 2014
Spatially organizing biochemistry: choosing a strategy to translate synthetic biology to the factory Jakobson, Christopher M.; Tullman-Ercek, Danielle; Mangan, Niall M. Scientific Reports, Vol. 8, Issue 1 https://doi.org/10.1038/s41598-018-26399-0	journal	May 2018
Synthetic protein scaffolds provide modular control over metabolic flux Dueber, John E.; Wu, Gabriel C.; Malmirchegini, G. Reza Nature Biotechnology, Vol. 27, Issue 8, p. 753-759 https://doi.org/10.1038/nbt.1557	journal	August 2009
Use of modular, synthetic scaffolds for improved production of glucaric acid in engineered E. coli Moon, Tae Seok; Dueber, John E.; Shiue, Eric Metabolic Engineering, Vol. 12, Issue 3 https://doi.org/10.1016/j.ymben.2010.01.003	journal	May 2010
Organization of Intracellular Reactions with Rationally Designed RNA Assemblies Delebecque, C. J.; Lindner, A. B.; Silver, P. A. Science, Vol. 333, Issue 6041 https://doi.org/10.1126/science.1206938	journal	June 2011
DNA-guided assembly of biosynthetic pathways promotes improved catalytic efficiency Conrado, Robert J.; Wu, Gabriel C.; Boock, Jason T. Nucleic Acids Research, Vol. 40, Issue 4 https://doi.org/10.1093/nar/gkr888	journal	October 2011
Solution Structure of a Bacterial Microcompartment Targeting Peptide and Its Application in the Construction of an Ethanol Bioreactor Lawrence, Andrew D.; Frank, Stefanie; Newnham, Sarah ACS Synthetic Biology, Vol. 3, Issue 7 https://doi.org/10.1021/sb4001118	journal	November 2013
A Catalytic Nanoreactor Based on in Vivo Encapsulation of Multiple Enzymes in an Engineered Protein Nanocompartment Giessen, Tobias W.; Silver, Pamela A. ChemBioChem, Vol. 17, Issue 20 https://doi.org/10.1002/cbic.201600431	journal	September 2016
Scaffoldless engineered enzyme assembly for enhanced methanol utilization Price, J. Vincent; Chen, Long; Whitaker, W. Brian Proceedings of the National Academy of Sciences, Vol. 113, Issue 45 https://doi.org/10.1073/pnas.1601797113	journal	October 2016
Enhanced itaconic acid production by self-assembly of two biosynthetic enzymes in Escherichia coli: Enhanced Itaconic Acid Production Yang, Zhongwei; Gao, Xin; Xie, Hui Biotechnology and Bioengineering, Vol. 114, Issue 2 https://doi.org/10.1002/bit.26081	journal	November 2016
CipA and CipB as Scaffolds To Organize Proteins into Crystalline Inclusions Wang, Yang; Heermann, Ralf; Jung, Kirsten ACS Synthetic Biology, Vol. 6, Issue 5 https://doi.org/10.1021/acssynbio.6b00323	journal	February 2017
Artificial Protein Scaffold System (AProSS): An efficient method to optimize exogenous metabolic pathways in Saccharomyces cerevisiae Li, Tianyi; Chen, Xiuqi; Cai, Yizhi Metabolic Engineering, Vol. 49 https://doi.org/10.1016/j.ymben.2018.07.006	journal	September 2018
Engineered synthetic scaffolds for organizing proteins within the bacterial cytoplasm Lee, Matthew J.; Mantell, Judith; Hodgson, Lorna Nature Chemical Biology, Vol. 14, Issue 2 https://doi.org/10.1038/nchembio.2535	journal	December 2017
Self-Assembling Protein Scaffold System for Easy in Vitro Coimmobilization of Biocatalytic Cascade Enzymes Zhang, Guoqiang; Quin, Maureen B.; Schmidt-Dannert, Claudia ACS Catalysis, Vol. 8, Issue 6 https://doi.org/10.1021/acscatal.8b00986	journal	May 2018
Enhanced Enzyme Activity through Scaffolding on Customizable Self‐Assembling Protein Filaments Lim, Samuel; Jung, Gi Ahn; Glover, Dominic J. Small, Vol. 15, Issue 20 https://doi.org/10.1002/smll.201805558	journal	March 2019
Engineering bacterial microcompartments with heterologous enzyme cargos Wagner, Hanna J.; Capitain, Charlotte C.; Richter, Karsten Engineering in Life Sciences, Vol. 17, Issue 1 https://doi.org/10.1002/elsc.201600107	journal	September 2016
Bacterial microcompartment-directed polyphosphate kinase promotes stable polyphosphate accumulation in E. coli Liang, Mingzhi; Frank, Stefanie; Lünsdorf, Heinrich Biotechnology Journal, Vol. 12, Issue 3 https://doi.org/10.1002/biot.201600415	journal	February 2017
Re-directing bacterial microcompartment systems to enhance recombinant expression of lysis protein E from bacteriophage ϕX174 in Escherichia coli Yung, Mimi C.; Bourguet, Feliza A.; Carpenter, Timothy S. Microbial Cell Factories, Vol. 16, Issue 1 https://doi.org/10.1186/s12934-017-0685-x	journal	April 2017
Programmed loading and rapid purification of engineered bacterial microcompartment shells Hagen, Andrew; Sutter, Markus; Sloan, Nancy Nature Communications, Vol. 9, Issue 1 https://doi.org/10.1038/s41467-018-05162-z	journal	July 2018
Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose Zhao, Xuebing; Zhang, Lihua; Liu, Dehua Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 4 https://doi.org/10.1002/bbb.1331	journal	March 2012
Genome analyses highlight the different biological roles of cellulases Medie, Felix Mba; Davies, Gideon J.; Drancourt, Michel Nature Reviews Microbiology, Vol. 10, Issue 3 https://doi.org/10.1038/nrmicro2729	journal	January 2012
Hemicelluloses Scheller, Henrik Vibe; Ulvskov, Peter Annual Review of Plant Biology, Vol. 61, Issue 1 https://doi.org/10.1146/annurev-arplant-042809-112315	journal	June 2010
Lignin Biosynthesis Boerjan, Wout; Ralph, John; Baucher, Marie Annual Review of Plant Biology, Vol. 54, Issue 1, p. 519-546 https://doi.org/10.1146/annurev.arplant.54.031902.134938	journal	June 2003
Pathways for degradation of lignin in bacteria and fungi Bugg, Timothy D. H.; Ahmad, Mark; Hardiman, Elizabeth M. Natural Product Reports, Vol. 28, Issue 12, p. 1883-1896 https://doi.org/10.1039/c1np00042j	journal	January 2011
Enzymatic hydrolysis of cellulosic biomass Yang, Bin; Dai, Ziyu; Ding, Shi-You Biofuels, Vol. 2, Issue 4 https://doi.org/10.4155/bfs.11.116	journal	July 2011
Biomass recalcitrance. Part II: Fundamentals of different pre-treatments to increase the enzymatic digestibility of lignocellulose: Part II: Fundamentals of different pre-treatments to increase the enzymatic digestibility of lignocellulose Zhao, Xuebing; Zhang, Lihua; Liu, Dehua Biofuels, Bioproducts and Biorefining, Vol. 6, Issue 5 https://doi.org/10.1002/bbb.1350	journal	June 2012
Synthetic enzyme mixtures for biomass deconstruction: Production and optimization of a core set Banerjee, Goutami; Car, Suzana; Scott-Craig, John S. Biotechnology and Bioengineering, Vol. 106, Issue 5 https://doi.org/10.1002/bit.22741	journal	March 2010
The cellulosome — A treasure-trove for biotechnology Bayer, Edward A.; Morag, Ely; Lamed, Raphael Trends in Biotechnology, Vol. 12, Issue 9 https://doi.org/10.1016/0167-7799(94)90039-6	journal	September 1994
Cellulosomes—Structure and Ultrastructure Bayer, Edward A.; Shimon, Linda J. W.; Shoham, Yuval Journal of Structural Biology, Vol. 124, Issue 2-3 https://doi.org/10.1006/jsbi.1998.4065	journal	December 1998
Cellulosomes: Highly Efficient Nanomachines Designed to Deconstruct Plant Cell Wall Complex Carbohydrates Fontes, Carlos M. G. A.; Gilbert, Harry J. Annual Review of Biochemistry, Vol. 79, Issue 1 https://doi.org/10.1146/annurev-biochem-091208-085603	journal	June 2010
Yeast Surface Display of Trifunctional Minicellulosomes for Simultaneous Saccharification and Fermentation of Cellulose to Ethanol Wen, F.; Sun, J.; Zhao, H. Applied and Environmental Microbiology, Vol. 76, Issue 4 https://doi.org/10.1128/AEM.01687-09	journal	December 2009
Dynamic interactions of type I cohesin modules fine-tune the structure of the cellulosome of Clostridium thermocellum Barth, Anders; Hendrix, Jelle; Fried, Daniel Proceedings of the National Academy of Sciences, Vol. 115, Issue 48 https://doi.org/10.1073/pnas.1809283115	journal	November 2018
Synergistic enhancement of cellulase pairs linked by consensus ankyrin repeats: Determination of the roles of spacing, orientation, and enzyme identity: Inserting Multiple Cellulases into Ankyrin Scaffolds Cunha, Eva S.; Hatem, Christine L.; Barrick, Doug Proteins: Structure, Function, and Bioinformatics, Vol. 84, Issue 8 https://doi.org/10.1002/prot.25047	journal	May 2016
Macromolecular Organization of the Cellulolytic Enzyme Complex of Clostridium thermocellum as Revealed by Electron Microscopy Mayer, Frank; Coughlan, Michael P.; Mori, Yutaka Applied and Environmental Microbiology, Vol. 53, Issue 12 https://doi.org/10.1128/AEM.53.12.2785-2792.1987	journal	January 1987
Enhanced Microbial Utilization of Recalcitrant Cellulose by an Ex Vivo Cellulosome-Microbe Complex You, Chun; Zhang, Xiao-Zhou; Sathitsuksanoh, Noppadon Applied and Environmental Microbiology, Vol. 78, Issue 5 https://doi.org/10.1128/AEM.07138-11	journal	December 2011
Enzyme-microbe synergy during cellulose hydrolysis by Clostridium thermocellum Lu, Y.; Zhang, Y. -H. P.; Lynd, L. R. Proceedings of the National Academy of Sciences, Vol. 103, Issue 44 https://doi.org/10.1073/pnas.0605381103	journal	October 2006
Cellulase, Clostridia, and Ethanol Demain, A. L.; Newcomb, M.; Wu, J. H. D. Microbiology and Molecular Biology Reviews, Vol. 69, Issue 1, p. 124-154 https://doi.org/10.1128/MMBR.69.1.124-154.2005	journal	March 2005
Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin Zakeri, B.; Fierer, J. O.; Celik, E. Proceedings of the National Academy of Sciences, Vol. 109, Issue 12 https://doi.org/10.1073/pnas.1115485109	journal	February 2012
Secrets of a covalent interaction for biomaterials and biotechnology: SpyTag and SpyCatcher Reddington, Samuel C.; Howarth, Mark Current Opinion in Chemical Biology, Vol. 29 https://doi.org/10.1016/j.cbpa.2015.10.002	journal	December 2015
Tuning SpyTag–SpyCatcher mutant pairs toward orthogonal reactivity encryption Liu, Yajie; Liu, Dong; Yang, Wei Chemical Science, Vol. 8, Issue 9 https://doi.org/10.1039/C7SC02686B	journal	January 2017
Active Template Synthesis of Protein Heterocatenanes Da, Xiao‐Di; Zhang, Wen‐Bin Angewandte Chemie International Edition, Vol. 58, Issue 32 https://doi.org/10.1002/anie.201904943	journal	August 2019
N-terminal cysteinyl proteins can be prepared using thrombin cleavage Liu, Dongsheng; Xu, Rong; Dutta, Kaushik FEBS Letters, Vol. 582, Issue 7 https://doi.org/10.1016/j.febslet.2008.02.078	journal	March 2008
N‐Terminal Methionine Processing Wingfield, Paul T. Current Protocols in Protein Science, Vol. 88, Issue 1 https://doi.org/10.1002/cpps.29	journal	April 2017
Site-Specific N- and C-Terminal Labeling of a Single Polypeptide Using Sortases of Different Specificity Antos, John M.; Chew, Guo-Liang; Guimaraes, Carla P. Journal of the American Chemical Society, Vol. 131, Issue 31 https://doi.org/10.1021/ja902681k	journal	July 2009
A general strategy for the evolution of bond-forming enzymes using yeast display Chen, I.; Dorr, B. M.; Liu, D. R. Proceedings of the National Academy of Sciences, Vol. 108, Issue 28 https://doi.org/10.1073/pnas.1101046108	journal	June 2011
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar Miller, G. L. Analytical Chemistry, Vol. 31, Issue 3 https://doi.org/10.1021/ac60147a030	journal	March 1959
Sortase A: A Model for Transpeptidation and Its Biological Applications Pishesha, Novalia; Ingram, Jessica R.; Ploegh, Hidde L. Annual Review of Cell and Developmental Biology, Vol. 34, Issue 1 https://doi.org/10.1146/annurev-cellbio-100617-062527	journal	October 2018
Sortases and the Art of Anchoring Proteins to the Envelopes of Gram-Positive Bacteria Marraffini, Luciano A.; DeDent, Andrea C.; Schneewind, Olaf Microbiology and Molecular Biology Reviews, Vol. 70, Issue 1 https://doi.org/10.1128/MMBR.70.1.192-221.2006	journal	March 2006
Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation Proft, Thomas Biotechnology Letters, Vol. 32, Issue 1 https://doi.org/10.1007/s10529-009-0116-0	journal	September 2009
Attachment of an NMR-invisible solubility enhancement tag using a sortase-mediated protein ligation method Kobashigawa, Yoshihiro; Kumeta, Hiroyuki; Ogura, Kenji Journal of Biomolecular NMR, Vol. 43, Issue 3 https://doi.org/10.1007/s10858-008-9296-5	journal	January 2009
Synthesis of Biologically Active Peptide Nucleic Acid−Peptide Conjugates by Sortase-Mediated Ligation Pritz, Stephan; Wolf, Yvonne; Kraetke, Oliver The Journal of Organic Chemistry, Vol. 72, Issue 10 https://doi.org/10.1021/jo062331l	journal	May 2007
Observing selected domains in multi-domain proteins via sortase-mediated ligation and NMR spectroscopy Refaei, Mary Anne; Combs, Al; Kojetin, Douglas J. Journal of Biomolecular NMR, Vol. 49, Issue 1 https://doi.org/10.1007/s10858-010-9464-2	journal	December 2010
How does cellulosome composition influence deconstruction of lignocellulosic substrates in Clostridium (Ruminiclostridium) thermocellum DSM 1313? Yoav, Shahar; Barak, Yoav; Shamshoum, Melina Biotechnology for Biofuels, Vol. 10, Issue 1 https://doi.org/10.1186/s13068-017-0909-7	journal	September 2017
Degradation of Cellulose Substrates by Cellulosome Chimeras: SUBSTRATE TARGETING Fierobe, Henri-Pierre; Bayer, Edward A.; Tardif, Chantal Journal of Biological Chemistry, Vol. 277, Issue 51 https://doi.org/10.1074/jbc.M207672200	journal	October 2002
Stoichiometric Assembly of the Cellulosome Generates Maximum Synergy for the Degradation of Crystalline Cellulose, as Revealed by In Vitro Reconstitution of the Clostridium thermocellum Cellulosome Hirano, Katsuaki; Nihei, Satoshi; Hasegawa, Hiroki Applied and Environmental Microbiology, Vol. 81, Issue 14 https://doi.org/10.1128/AEM.00772-15	journal	July 2015
Design and Production of Active Cellulosome Chimeras: SELECTIVE INCORPORATION OF DOCKERIN-CONTAINING ENZYMES INTO DEFINED FUNCTIONAL COMPLEXES Fierobe, Henri-Pierre; Mechaly, Adva; Tardif, Chantal Journal of Biological Chemistry, Vol. 276, Issue 24 https://doi.org/10.1074/jbc.M102082200	journal	April 2001
Action of Designer Cellulosomes on Homogeneous Versus Complex Substrates : CONTROLLED INCORPORATION OF THREE DISTINCT ENZYMES INTO A DEFINED TRIFUNCTIONAL SCAFFOLDIN Fierobe, Henri-Pierre; Mingardon, Florence; Mechaly, Adva Journal of Biological Chemistry, Vol. 280, Issue 16 https://doi.org/10.1074/jbc.M414449200	journal	February 2005
In Vitro Reconstitution of the Complete Clostridium thermocellum Cellulosome and Synergistic Activity on Crystalline Cellulose Krauss, Jan; Zverlov, Vladimir V.; Schwarz, Wolfgang H. Applied and Environmental Microbiology, Vol. 78, Issue 12 https://doi.org/10.1128/AEM.07959-11	journal	April 2012
Enhanced cellulose degradation by nano-complexed enzymes: Synergism between a scaffold-linked exoglucanase and a free endoglucanase Moraïs, Sarah; Heyman, Arnon; Barak, Yoav Journal of Biotechnology, Vol. 147, Issue 3-4 https://doi.org/10.1016/j.jbiotec.2010.04.012	journal	June 2010
The rosettazyme: A synthetic cellulosome Mitsuzawa, Shigenobu; Kagawa, Hiromi; Li, Yifen Journal of Biotechnology, Vol. 143, Issue 2 https://doi.org/10.1016/j.jbiotec.2009.06.019	journal	August 2009
Deletion of the Cel48S cellulase from Clostridium thermocellum Olson, Daniel G.; Tripathi, Shital A.; Giannone, Richard J. Proceedings of the National Academy of Sciences, Vol. 107, Issue 41, p. 17727-17732 https://doi.org/10.1073/pnas.1003584107	journal	September 2010
Enhanced cellulose degradation by targeted integration of a cohesin-fused -glucosidase into the Clostridium thermocellum cellulosome Gefen, G.; Anbar, M.; Morag, E. Proceedings of the National Academy of Sciences, Vol. 109, Issue 26 https://doi.org/10.1073/pnas.1202747109	journal	June 2012
Cellulose-binding domains promote hydrolysis of different sites on crystalline cellulose Carrard, G.; Koivula, A.; Soderlund, H. Proceedings of the National Academy of Sciences, Vol. 97, Issue 19 https://doi.org/10.1073/pnas.160216697	journal	August 2000
Enzymatic diversity of the Clostridium thermocellum cellulosome is crucial for the degradation of crystalline cellulose and plant biomass Hirano, Katsuaki; Kurosaki, Masahiro; Nihei, Satoshi Scientific Reports, Vol. 6, Issue 1 https://doi.org/10.1038/srep35709	journal	October 2016
Oxidative coupling of peptides to a virus capsid containing unnatural amino acids Carrico, Zachary M.; Romanini, Dante W.; Mehl, Ryan A. Chemical Communications, Issue 10 https://doi.org/10.1039/b717826c	journal	January 2008
Self-Assembling Light-Harvesting Systems from Synthetically Modified Tobacco Mosaic Virus Coat Proteins Miller, Rebekah A.; Presley, Andrew D.; Francis, Matthew B. Journal of the American Chemical Society, Vol. 129, Issue 11 https://doi.org/10.1021/ja063887t	journal	March 2007
Nanoreactors by Programmed Enzyme Encapsulation Inside the Capsid of the Bacteriophage P22 Patterson, Dustin P.; Prevelige, Peter E.; Douglas, Trevor ACS Nano, Vol. 6, Issue 6 https://doi.org/10.1021/nn300545z	journal	May 2012
Encapsulation of an Enzyme Cascade within the Bacteriophage P22 Virus-Like Particle Patterson, Dustin P.; Schwarz, Benjamin; Waters, Ryan S. ACS Chemical Biology, Vol. 9, Issue 2 https://doi.org/10.1021/cb4006529	journal	November 2013
Influence of Electrostatics on Small Molecule Flux through a Protein Nanoreactor Glasgow, Jeff E.; Asensio, Michael A.; Jakobson, Christopher M. ACS Synthetic Biology, Vol. 4, Issue 9 https://doi.org/10.1021/acssynbio.5b00037	journal	April 2015
Synthetically Modified Viral Capsids as Versatile Carriers for Use in Antibody-Based Cell Targeting ElSohly, Adel M.; Netirojjanakul, Chawita; Aanei, Ioana L. Bioconjugate Chemistry, Vol. 26, Issue 8 https://doi.org/10.1021/acs.bioconjchem.5b00226	journal	July 2015
Directed Evolution of a Protein Container Worsdorfer, B.; Woycechowsky, K. J.; Hilvert, D. Science, Vol. 331, Issue 6017 https://doi.org/10.1126/science.1199081	journal	February 2011

Similar Records

Design and Characterization of an Icosahedral Protein Cage Formed by a Double-Fusion Protein Containing Three Distinct Symmetry Elements

Journal Article · Wed Feb 12 00:00:00 EST 2020 · ACS Synthetic Biology · OSTI ID:1799610

Cannon, Kevin A.; Nguyen, Vy N.; Morgan, Christian; +1 more

Designing and defining dynamic protein cage nanoassemblies in solution

Journal Article · Wed Dec 14 00:00:00 EST 2016 · Science Advances · OSTI ID:1799610

Lai, Y. -T.; Hura, G. L.; Dyer, K. N.; +3 more

Design of a symmetry‐broken tetrahedral protein cage by a method of internal steric occlusion

Journal Article · Wed Mar 27 00:00:00 EDT 2024 · Protein Science · OSTI ID:1799610

Gladkov, Nika; Scott, Elena A.; Meador, Kyle; +4 more

Related Subjects

Biochemistry & Molecular Biology

Title: Designed Protein Cages as Scaffolds for Building Multienzyme Materials

Citation Formats

References (93)

Similar Records

Related Subjects