Nanostructure Imaging Mass Spectrometry: The Role of Fluorocarbons in Metabolite Analysis and Yoctomole Level Sensitivity

Kurczy, Michael E.; Northen, Trent R.; Trauger, Sunia A.; Siuzdak, Gary

doi:10.1007/978-1-4939-1357-2_14

Nanostructure Imaging Mass Spectrometry: The Role of Fluorocarbons in Metabolite Analysis and Yoctomole Level Sensitivity

Book · Fri Oct 17 04:00:00 EDT 2014 · Methods in Molecular Biology

DOI:https://doi.org/10.1007/978-1-4939-1357-2_14· OSTI ID:1788439

Kurczy, Michael E. ^[1]; Northen, Trent R. ^[2]; Trauger, Sunia A. ^[3]; Siuzdak, Gary ^[1]

The Scripps Research Inst., La Jolla, CA (United States)
Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)
Harvard Univ., Cambridge, MA (United States)

Nanostructure Imaging mass spectrometry (NIMS) has become an effective technology for generating ions in the gas phase, providing high sensitivity and imaging capabilities on small molecules, metabolites, drugs, and drug metabolites. Specifically, laser desorption from the nanostructure surfaces results in efficient energy transfer, low background chemical noise, and the nondestructive release of analyte ions into the gas phase. The modification of nanostructured surfaces with fluorous compounds, either covalent or non-covalent, has played an important role in gaining high efficiency/sensitivity by facilitating analyte desorption from the non-adhesive surfaces, and minimizing the amount of laser energy required. In addition, the hydrophobic fluorinated nanostructure surfaces have aided in concentrating deposited samples into fine micrometer sized spots, a feature that further facilitates efficient desorption/ionization. These fluorous nanostructured surfaces have opened up NIMS to very broad applications including enzyme activity assays and imaging, providing low background, efficient energy transfer, nondestructive analyte ion generation, super-hydrophobic surfaces, and ultra-high detection sensitivity

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Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC), Biological and Environmental Research (BER)

DOE Contract Number:: AC02-05CH11231

OSTI ID:: 1788439

Country of Publication:: United States

Language:: English

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