Comparative genomics, proteomics and transcriptomics give new insight into the exoproteome of the basidiomycete H ebeloma cylindrosporum and its involvement in ectomycorrhizal symbiosis

Doré, Jeanne; Perraud, Marie; Dieryckx, Cindy; Kohler, Annegret; Morin, Emmanuelle; Henrissat, Bernard; Lindquist, Erika; Zimmermann, Sabine D.; Girard, Vincent; Kuo, Alan; Grigoriev, Igor V.; Martin, Francis; Marmeisse, Roland; Gay, Gilles

doi:10.1111/nph.13546

Comparative genomics, proteomics and transcriptomics give new insight into the exoproteome of the basidiomycete H ebeloma cylindrosporum and its involvement in ectomycorrhizal symbiosis

Journal Article · Tue Jul 14 00:00:00 EDT 2015 · New Phytologist

DOI:https://doi.org/10.1111/nph.13546· OSTI ID:1787127

Doré, Jeanne ^[1]; Perraud, Marie ^[1]; Dieryckx, Cindy ^[2]; Kohler, Annegret ^[3]; Morin, Emmanuelle ^[3]; Henrissat, Bernard ^[4]; Lindquist, Erika ^[5]; Zimmermann, Sabine D. ^[6]; Girard, Vincent ^[2]; Kuo, Alan ^[5]; Grigoriev, Igor V. ^[5]; Martin, Francis ^[3]; Marmeisse, Roland ^[1]; Gay, Gilles ^[1]

Ecologie Microbienne UMR CNRS 5557 USC INRA 1364 Université Lyon 1 Université de Lyon F‐69622 Villeurbanne France
Proteomics Platform UMR5240 14 impasse Pierre Baizet BP 99163 F‐69263 Lyon Cedex 09 France
INRA UMR 1136 INRA‐Université de Lorraine Interactions Arbres/Microorganismes INRA‐Nancy 54280 Champenoux France
Centre National de la Recherche Scientifique UMR 7257 Aix‐Marseille Université 13288 Marseille Cedex 9 France
US Department of Energy Joint Genome Institute Walnut Creek CA 94598 USA
Biochimie et Physiologie Moléculaire des Plantes UMR 5004 CNRS/INRA/SupAgro/UM2 Place Viala Montpellier France

Summary

Extracellular proteins play crucial roles in the interaction between mycorrhizal fungi and their environment. Computational prediction and experimental detection allowed identification of 869 proteins constituting the exoproteome of Hebeloma cylindrosporum . Small secreted proteins ( SSP s) and carbohydrate‐active enzymes ( CAZ ymes) were the two major classes of extracellular proteins.

Twenty‐eight per cent of the SSP s were secreted by free‐living mycelia and five of the 10 most abundant extracellular proteins were SSP s. By contrast, 63–75% of enzymes involved in nutrient acquisition were secreted.

A total of 150 extracellular protein‐coding genes were differentially expressed between mycorrhizas and free‐living mycelia. SSP s were the most affected. External environmental conditions also affected expression of 199 exoproteome genes in mycorrhizas. SSP s displayed different patterns of regulation in response to presence of a host plant or other environmental signals. Several of the genes most overexpressed in the presence of organic matter encoded oxidoreductases.

Hebeloma cylindrosporum has not fully lost its ancestral saprotrophic capacities but rather adapted them not to harm its hosts and to use soil organic nitrogen. The complex and divergent patterns of regulation of SSP s in response to a symbiotic partner and/or organic matter suggest various roles in the biology of mycorrhizal fungi.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1787127

Journal Information:: New Phytologist, Journal Name: New Phytologist Journal Issue: 4 Vol. 208; ISSN 0028-646X

Publisher:: Wiley-BlackwellCopyright Statement

Country of Publication:: United Kingdom

Language:: English

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