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Engineered Root Bacteria Release Plant-Available Phosphate from Phytate

Journal Article · · Applied and Environmental Microbiology
DOI:https://doi.org/10.1128/AEM.01210-19· OSTI ID:1785305
 [1];  [1];  [1];  [1];  [1];  [1];  [1];  [1];
  1. Department of Energy Joint Genome Institute, Walnut Creek, California, USA
+ Show Author Affiliations

Phosphate fertilizers are essential for high-yield agriculture yet are costly and environmentally damaging. Microbes that release soluble phosphate from naturally occurring sources in the soil are appealing, as they may reduce the need for such fertilizers. In this study, we used synthetic biology approaches to create a collection of engineered root-associated microbes with the ability to release phosphate from phytate. We demonstrate that these strains improve plant growth under phosphorus-limited conditions. This represents a first step in the development of phosphate-mining bacteria for future use in crop systems.

Sponsoring Organization:
USDOE
Grant/Contract Number:
NONE; AC02-05CH11231
OSTI ID:
1785305
Alternate ID(s):
OSTI ID: 1580921
Journal Information:
Applied and Environmental Microbiology, Journal Name: Applied and Environmental Microbiology Journal Issue: 18 Vol. 85; ISSN 0099-2240
Publisher:
American Society for MicrobiologyCopyright Statement
Country of Publication:
United States
Language:
English

References (40)

Innovative methods in soil phosphorus research: A review journal January 2015
A new method for estimating gross phosphorus mineralization and immobilization rates in soils journal December 1992
Gluconic acid production and phosphate solubilization by the plant growth-promoting bacterium Azospirillum spp. journal October 2004
Heterologous expression of pyrroloquinoline quinone (pqq) gene cluster confers mineral phosphate solubilization ability to Herbaspirillum seropedicae Z67 journal February 2014
Isolation of a Gene from Burkholderia cepacia IS-16 Encoding a Protein That Facilitates Phosphatase Activity journal June 2000
Genetics of phosphate solubilization and its potential applications for improving plant growth-promoting bacteria journal September 2006
Phosphorus in soils and plants – facing phosphorus scarcity journal March 2016
Opportunities for mobilizing recalcitrant phosphorus from agricultural soils: a review journal August 2017
Development of an Engineered Soil Bacterium Enabling to Convert Both Insoluble Inorganic and Organic Phosphate into Plant Available Phosphate and Its Use as a Biofertilizer journal January 2015
Characterization of phosphate-solubilizing microorganisms from salt-affected soils of India and their effect on growth of sorghum plants [Sorghum bicolor (L.) Moench] journal February 2011
Comparative effectiveness of Bacillus spp. possessing either dual or single growth-promoting traits for improving phosphorus uptake, growth and yield of wheat (Triticum aestivum L.) journal September 2011
Survival of Pseudomonas putida KT2440 in soil and in the rhizosphere of plants under greenhouse and environmental conditions journal March 2000
Expression of a mineral phosphate solubilizing gene from Erwinia herbicola in two rhizobacterial strains journal November 2000
Phosphate solubilizing bacteria and their role in plant growth promotion journal October 1999
Fresh-weight measurements of roots provide inaccurate estimates of the effects of plant growth-promoting bacteria on root growth: a critical examination journal October 2005
Plant assimilation of phosphorus from an insoluble organic form is improved by addition of an organic anion producing Pseudomonas sp. journal January 2014
Ten Years of Maintaining and Expanding a Microbial Genome and Metagenome Analysis System journal November 2015
The rhizosphere microbiome and plant health journal August 2012
Streamlining the Design-to-Build Transition with Build-Optimization Software Tools journal November 2016
Phylogenomically Guided Identification of Industrially Relevant GH1 β-Glucosidases through DNA Synthesis and Nanostructure-Initiator Mass Spectrometry journal July 2014
A phylogeny-driven genomic encyclopaedia of Bacteria and Archaea journal December 2009
Engineering phosphorus metabolism in plants to produce a dual fertilization and weed control system journal August 2012
The phosphorus trilemma journal October 2013
Prospects for using soil microorganisms to improve the acquisition of phosphorus by plants journal January 2001
MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability journal January 2013
IMG/M: integrated genome and metagenome comparative data analysis system journal October 2016
Inositol phosphates in the environment
  • Turner, Benjamin L.; Papházy, Michael J.; Haygarth, Philip M.
  • Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, Vol. 357, Issue 1420 https://doi.org/10.1098/rstb.2001.0837
journal April 2002
Soil Microorganisms Mediating Phosphorus Availability Update on Microbial Phosphorus journal May 2011
A Comparison of the Sorption of Inorganic Orthophosphate and Inositol Hexaphosphate by six acid Soils journal March 1974
Plant growth promotion abilities and microscale bacterial dynamics in the rhizosphere of Lupin analysed by phytate utilization ability journal March 2005
Isolation and assessment of phytate-hydrolysing bacteria from the DelMarVa Peninsula journal December 2007
An efficient microbiological growth medium for screening phosphate solubilizing microorganisms journal January 1999
The secretion of the bacterial phytase PHY-US417 by Arabidopsis roots reveals its potential for increasing phosphate acquisition and biomass production during co-growth journal March 2016
Characterization and overproduction of the Escherichia coli appA encoded bifunctional enzyme that exhibits both phytase and acid phosphatase activities journal January 2000
Plant growth responses to bacterization with selected Pseudomonas spp. strains and rhizosphere microbial development in hydroponic cultures journal April 1989
Soil isolates of Pseudomonas spp. that utilize inositol phosphates journal June 1997
Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils journal October 2013
FastTree 2 – Approximately Maximum-Likelihood Trees for Large Alignments journal March 2010
Artificial Citrate Operon Confers Mineral Phosphate Solubilization Ability to Diverse Fluorescent Pseudomonads journal September 2014
Effect of Transgenic Rhizobacteria Overexpressing Citrobacter braakii appA on Phytate-P Availability to Mung Bean Plants journal November 2010

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