Allosteric HIV Integrase Inhibitors Promote Formation of Inactive Branched Polymers via Homomeric Carboxy-Terminal Domain Interactions

Gupta, K; Allen, A.; Giraldo, C.; Eilers, G.; Sharp, R.; Hwang, Y.; Murali, H.; Cruz, K.; Janmey, P.; Bushman, F.; Van Duyne, G.

doi:10.1016/j.str.2020.12.001

Title: Allosteric HIV Integrase Inhibitors Promote Formation of Inactive Branched Polymers via Homomeric Carboxy-Terminal Domain Interactions

Journal Article · 01 January 2022 · Structure

DOI:https://doi.org/10.1016/j.str.2020.12.001· OSTI ID:1783997

Gupta, K; Allen, A.; Giraldo, C.; Eilers, G.; Sharp, R.; Hwang, Y.; Murali, H.; Cruz, K.; Janmey, P.; Bushman, F.; Van Duyne, G.

Cite

Export

Save

Research Organization:: Brookhaven National Laboratory (BNL), Upton, NY (United States)

Sponsoring Organization:: ~OTHER

DOE Contract Number:: SC0012704

OSTI ID:: 1783997

Report Number(s):: BNL-221528-2021-JACI

Journal Information:: Structure, Vol. 29, Issue 3

Country of Publication:: United States

Language:: English

References (88)

Identification of cellular factors binding to acetylated HIV-1 integrase Allouch, Awatef; Cereseto, Anna Amino Acids, Vol. 41, Issue 5 https://doi.org/10.1007/s00726-009-0444-3	journal	December 2009
The TRIM Family Protein KAP1 Inhibits HIV-1 Integration Allouch, Awatef; Di Primio, Cristina; Alpi, Emanuele Cell Host & Microbe, Vol. 9, Issue 6 https://doi.org/10.1016/j.chom.2011.05.004	journal	June 2011
HIV-1 Antiretroviral Drug Therapy Arts, E. J.; Hazuda, D. J. Cold Spring Harbor Perspectives in Medicine, Vol. 2, Issue 4 https://doi.org/10.1101/cshperspect.a007161	journal	January 2012
Non-Catalytic Site HIV-1 Integrase Inhibitors Disrupt Core Maturation and Induce a Reverse Transcription Block in Target Cells Balakrishnan, Mini; Yant, Stephen R.; Tsai, Luong PLoS ONE, Vol. 8, Issue 9 https://doi.org/10.1371/journal.pone.0074163	journal	September 2013
Small-Angle X-Ray-Scattering Investigation of Submicroscopic Porosity with Fractal Properties Bale, Harold D.; Schmidt, Paul W. Physical Review Letters, Vol. 53, Issue 6 https://doi.org/10.1103/PhysRevLett.53.596	journal	August 1984
A supramolecular assembly mediates lentiviral DNA integration Ballandras-Colas, Allison; Maskell, Daniel P.; Serrao, Erik Science, Vol. 355, Issue 6320 https://doi.org/10.1126/science.aah7002	journal	January 2017
An Hsp60 related protein is associated with purified HIV and SIV Bartz, Steven R.; Pauza, C. David; Ivanyi, Juraj Journal of Medical Primatology, Vol. 23, Issue 2-3 https://doi.org/10.1111/j.1600-0684.1994.tb00116.x	journal	February 1994
Three-dimensional Structure of HIV-1 Virus-like Particles by Electron Cryotomography Benjamin, Jordan; Ganser-Pornillos, Barbie K.; Tivol, William F. Journal of Molecular Biology, Vol. 346, Issue 2 https://doi.org/10.1016/j.jmb.2004.11.064	journal	February 2005
Characterization of the neutron detector upgrade to the GP-SANS and Bio-SANS instruments at HFIR Berry, Kevin D.; Bailey, Katherine M.; Beal, Justin Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, Vol. 693 https://doi.org/10.1016/j.nima.2012.06.052	journal	November 2012
Association Equilibrium of the HIV-1 Capsid Protein in a Crowded Medium Reveals that Hexamerization during Capsid Assembly Requires a Functional C-Domain Dimerization Interface Bocanegra, Rebeca; Alfonso, Carlos; Rodríguez-Huete, Alicia Biophysical Journal, Vol. 104, Issue 4 https://doi.org/10.1016/j.bpj.2012.12.035	journal	February 2013
Calculations and Publication-Quality Illustrations for Analytical Ultracentrifugation Data Brautigam, Chad A. Methods in Enzymology https://doi.org/10.1016/bs.mie.2015.05.001	book	January 2015
The stoichiometry of Gag protein in HIV-1 Briggs, John A. G.; Simon, Martha N.; Gross, Ingolf Nature Structural & Molecular Biology, Vol. 11, Issue 7 https://doi.org/10.1038/nsmb785	journal	June 2004
F-actin and Microtubule Suspensions as Indeterminate Fluids Buxbaum, Robert E.; Dennerll, Timothy; Weiss, Serge Science, Vol. 235, Issue 4795 https://doi.org/10.1126/science.2881354	journal	March 1987
Solution structure of the His 12 → Cys mutant of the N-terminal zinc binding domain of HIV-1 integrase complexed to cadmium Cai, Mengli; Caffrey, Michael; Clore, G. Marius Protein Science, Vol. 7, Issue 12 https://doi.org/10.1002/pro.5560071221	journal	December 1998
Solution structure of the N-terminal zinc binding domain of HIV-1 integrase Cai, Mengli; Zheng, Ronglan; Caffrey, Michael Nature Structural Biology, Vol. 4, Issue 7 https://doi.org/10.1038/nsb0797-567	journal	July 1997
Acetylation of HIV-1 integrase by p300 regulates viral integration Cereseto, Anna; Manganaro, Lara; Gutierrez, Maria Ines The EMBO Journal, Vol. 24, Issue 17 https://doi.org/10.1038/sj.emboj.7600770	journal	August 2005
Rheology of Soft Materials Chen, Daniel T. N.; Wen, Qi; Janmey, Paul A. Annual Review of Condensed Matter Physics, Vol. 1, Issue 1 https://doi.org/10.1146/annurev-conmatphys-070909-104120	journal	August 2010
Crystal structure of the HIV-1 integrase catalytic core and C-terminal domains: A model for viral DNA binding Chen, J. C. -H.; Krucinski, J.; Miercke, L. J. W. Proceedings of the National Academy of Sciences, Vol. 97, Issue 15 https://doi.org/10.1073/pnas.150220297	journal	July 2000
Rational design of small-molecule inhibitors of the LEDGF/p75-integrase interaction and HIV replication Christ, Frauke; Voet, Arnout; Marchand, Arnaud Nature Chemical Biology, Vol. 6, Issue 6 https://doi.org/10.1038/nchembio.370	journal	May 2010
Current status and prospects of HIV treatment Cihlar, Tomas; Fordyce, Marshall Current Opinion in Virology, Vol. 18 https://doi.org/10.1016/j.coviro.2016.03.004	journal	June 2016
Modulating Target Site Selection During Human Immunodeficiency Virus DNA Integration In Vitro with an Engineered Tethering Factor Ciuffi, Angela; Diamond, Tracy L.; Hwang, Young Human Gene Therapy, Vol. 17, Issue 9 https://doi.org/10.1089/hum.2006.17.960	journal	September 2006
A role for LEDGF/p75 in targeting HIV DNA integration Ciuffi, Angela; Llano, Manuel; Poeschla, Eric Nature Medicine, Vol. 11, Issue 12 https://doi.org/10.1038/nm1329	journal	November 2005
HIV DNA Integration Craigie, R.; Bushman, F. D. Cold Spring Harbor Perspectives in Medicine, Vol. 2, Issue 7 https://doi.org/10.1101/cshperspect.a006890	journal	May 2012
Effect of Macromolecular Crowding Agents on Human Immunodeficiency Virus Type 1 Capsid Protein Assembly In Vitro del Álamo, Marta; Rivas, Germán; Mateu, Mauricio G. Journal of Virology, Vol. 79, Issue 22 https://doi.org/10.1128/JVI.79.22.14271-14281.2005	journal	November 2005
Dynamic microtubules at the synapse Dent, Erik W. Current Opinion in Neurobiology, Vol. 63 https://doi.org/10.1016/j.conb.2020.01.003	journal	August 2020
Oligomeric States of the HIV-1 Integrase As Measured by Time-Resolved Fluorescence Anisotropy ^† Deprez, Eric; Tauc, Patrick; Leh, Hervé Biochemistry, Vol. 39, Issue 31 https://doi.org/10.1021/bi000397j	journal	August 2000
DNA binding induces dissociation of the multimeric form of HIV-1 integrase: A time-resolved fluorescence anisotropy study Deprez, E.; Tauc, P.; Leh, H. Proceedings of the National Academy of Sciences, Vol. 98, Issue 18 https://doi.org/10.1073/pnas.181024498	journal	August 2001
LEDGINs inhibit late stage HIV-1 replication by modulating integrase multimerization in the virions Desimmie, Belete Ayele; Schrijvers, Rik; Demeulemeester, Jonas Retrovirology, Vol. 10, Issue 1 https://doi.org/10.1186/1742-4690-10-57	journal	May 2013
Role of metal ions in catalysis by HIV integrase analyzed using a quantitative PCR disintegration assay Diamond, Tracy L.; Bushman, Frederic D. Nucleic Acids Research, Vol. 34, Issue 21 https://doi.org/10.1093/nar/gkl862	journal	November 2006
The life science x-ray scattering beamline at NSLS-II DiFabio, Jonathan; Chodankar, Shirish; Pjerov, Sal PROCEEDINGS OF THE 12TH INTERNATIONAL CONFERENCE ON SYNCHROTRON RADIATION INSTRUMENTATION – SRI2015, AIP Conference Proceedings https://doi.org/10.1063/1.4952872	conference	January 2016
Crystal Structure of the Catalytic Domain of HIV-1 Integrase: Similarity to Other Polynucleotidyl Transferases Dyda, Fred; Hickman, Alison B.; Jenkins, Timothy M. Science, Vol. 266, Issue 5193 https://doi.org/10.1126/science.7801124	journal	December 1994
Refined solution structure of the c-terminal DNA-binding domain of human immunovirus-1 integrase Eijkelenboom, Astrid P. A. M.; Sprangers, Remco; H�rd, Karl Proteins: Structure, Function, and Genetics, Vol. 36, Issue 4 https://doi.org/10.1002/(SICI)1097-0134(19990901)36:4<556::AID-PROT18>3.0.CO;2-6	journal	September 1999
Über die von der molekularkinetischen Theorie der Wärme geforderte Bewegung von in ruhenden Flüssigkeiten suspendierten Teilchen Einstein, A. Annalen der Physik, Vol. 322, Issue 8 https://doi.org/10.1002/andp.19053220806	journal	January 1905
The structural biology of HIV-1: mechanistic and therapeutic insights Engelman, Alan; Cherepanov, Peter Nature Reviews Microbiology, Vol. 10, Issue 4 https://doi.org/10.1038/nrmicro2747	journal	March 2012
Allosteric inhibition of HIV-1 integrase activity Engelman, Alan; Kessl, Jacques J.; Kvaratskhelia, Mamuka Current Opinion in Chemical Biology, Vol. 17, Issue 3 https://doi.org/10.1016/j.cbpa.2013.04.010	journal	June 2013
Structure-based mutagenesis of the catalytic domain of human immunodeficiency virus type 1 integrase. Engelman, A.; Liu, Y.; Chen, H. Journal of virology, Vol. 71, Issue 5 https://doi.org/10.1128/jvi.71.5.3507-3514.1997	journal	January 1997
Aligning Potency and Pharmacokinetic Properties for Pyridine-Based NCINIs Fader, Lee D.; Bailey, Murray; Beaulieu, Eric ACS Medicinal Chemistry Letters, Vol. 7, Issue 8 https://doi.org/10.1021/acsmedchemlett.6b00194	journal	June 2016
The Competitive Interplay between Allosteric HIV-1 Integrase Inhibitor BI/D and LEDGF/p75 during the Early Stage of HIV-1 Replication Adversely Affects Inhibitor Potency Feng, Lei; Dharmarajan, Venkatasubramanian; Serrao, Erik ACS Chemical Biology, Vol. 11, Issue 5 https://doi.org/10.1021/acschembio.6b00167	journal	March 2016
The A128T Resistance Mutation Reveals Aberrant Protein Multimerization as the Primary Mechanism of Action of Allosteric HIV-1 Integrase Inhibitors Feng, Lei; Sharma, Amit; Slaughter, Alison Journal of Biological Chemistry, Vol. 288, Issue 22 https://doi.org/10.1074/jbc.M112.443390	journal	April 2013
ATSAS 2.8 : a comprehensive data analysis suite for small-angle scattering from macromolecular solutions Franke, D.; Petoukhov, M. V.; Konarev, P. V. Journal of Applied Crystallography, Vol. 50, Issue 4 https://doi.org/10.1107/S1600576717007786	journal	June 2017
Three new structures of the core domain of HIV-1 integrase: An active site that binds magnesium Goldgur, Yehuda; Dyda, Fred; Hickman, Alison B. Proceedings of the National Academy of Sciences, Vol. 95, Issue 16 https://doi.org/10.1073/pnas.95.16.9150	journal	August 1998
Allosteric Inhibition of Human Immunodeficiency Virus Integrase: LATE BLOCK DURING VIRAL REPLICATION AND ABNORMAL MULTIMERIZATION INVOLVING SPECIFIC PROTEIN DOMAINS Gupta, Kushol; Brady, Troy; Dyer, Benjamin M. Journal of Biological Chemistry, Vol. 289, Issue 30 https://doi.org/10.1074/jbc.M114.551119	journal	June 2014
Structural Properties of HIV Integrase·Lens Epithelium-derived Growth Factor Oligomers Gupta, Kushol; Diamond, Tracy; Hwang, Young Journal of Biological Chemistry, Vol. 285, Issue 26 https://doi.org/10.1074/jbc.M110.114413	journal	April 2010
Coiled-coil interactions mediate serine integrase directionality Gupta, Kushol; Sharp, Robert; Yuan, Jimmy B. Nucleic Acids Research, Vol. 45, Issue 12 https://doi.org/10.1093/nar/gkx474	journal	May 2017
Structural Basis for Inhibitor-Induced Aggregation of HIV Integrase Gupta, Kushol; Turkki, Vesa; Sherrill-Mix, Scott PLOS Biology, Vol. 14, Issue 12 https://doi.org/10.1371/journal.pbio.1002584	journal	December 2016
Structural Basis for Functional Tetramerization of Lentiviral Integrase Hare, Stephen; Di Nunzio, Francesca; Labeja, Alfred PLoS Pathogens, Vol. 5, Issue 7 https://doi.org/10.1371/journal.ppat.1000515	journal	July 2009
A Novel Co-Crystal Structure Affords the Design of Gain-of-Function Lentiviral Integrase Mutants in the Presence of Modified PSIP1/LEDGF/p75 Hare, Stephen; Shun, Ming-Chieh; Gupta, Saumya Shree PLoS Pathogens, Vol. 5, Issue 1 https://doi.org/10.1371/journal.ppat.1000259	journal	January 2009
Structural and Functional Analyses of the Second-Generation Integrase Strand Transfer Inhibitor Dolutegravir (S/GSK1349572) Hare, Stephen; Smith, Steven J.; Métifiot, Mathieu Molecular Pharmacology, Vol. 80, Issue 4 https://doi.org/10.1124/mol.111.073189	journal	June 2011
The Bio-SANS instrument at the High Flux Isotope Reactor of Oak Ridge National Laboratory Heller, William T.; Urban, Volker S.; Lynn, Gary W. Journal of Applied Crystallography, Vol. 47, Issue 4 https://doi.org/10.1107/S1600576714011285	journal	June 2014
BioXTAS RAW : improvements to a free open-source program for small-angle X-ray scattering data reduction and analysis Hopkins, Jesse Bennett; Gillilan, Richard E.; Skou, Soren Journal of Applied Crystallography, Vol. 50, Issue 5 https://doi.org/10.1107/S1600576717011438	journal	September 2017
VMD: Visual molecular dynamics Humphrey, William; Dalke, Andrew; Schulten, Klaus Journal of Molecular Graphics, Vol. 14, Issue 1 https://doi.org/10.1016/0263-7855(96)00018-5	journal	February 1996
A Soluble Active Mutant of HIV-1 Integrase: INVOLVEMENT OF BOTH THE CORE AND CARBOXYL-TERMINAL DOMAINS IN MULTIMERIZATION Jenkins, Timothy M.; Engelman, Alan; Ghirlando, Rodolfo Journal of Biological Chemistry, Vol. 271, Issue 13 https://doi.org/10.1074/jbc.271.13.7712	journal	March 1996
Allosteric integrase inhibitor potency is determined through the inhibition of HIV-1 particle maturation Jurado, K. A.; Wang, H.; Slaughter, A. Proceedings of the National Academy of Sciences, Vol. 110, Issue 21 https://doi.org/10.1073/pnas.1300703110	journal	April 2013
HIV-1 Integrase Binds the Viral RNA Genome and Is Essential during Virion Morphogenesis Kessl, Jacques J.; Kutluay, Sebla B.; Townsend, Dana Cell, Vol. 166, Issue 5 https://doi.org/10.1016/j.cell.2016.07.044	journal	August 2016
HIV-1 Tat transcriptional activity is regulated by acetylation Kiernan, R. E. The EMBO Journal, Vol. 18, Issue 21 https://doi.org/10.1093/emboj/18.21.6106	journal	November 1999
Acetylation regulates Cyclophilin A catalysis, immunosuppression and HIV isomerization Lammers, Michael; Neumann, Heinz; Chin, Jason W. Nature Chemical Biology, Vol. 6, Issue 5 https://doi.org/10.1038/nchembio.342	journal	April 2010
Interaction of the HIV-1 Intasome with Transportin 3 Protein (TNPO3 or TRN-SR2) Larue, Ross; Gupta, Kushol; Wuensch, Christiane Journal of Biological Chemistry, Vol. 287, Issue 41 https://doi.org/10.1074/jbc.M112.384669	journal	October 2012
Dual inhibition of HIV-1 replication by integrase-LEDGF allosteric inhibitors is predominant at the post-integration stage Le Rouzic, Erwann; Bonnard, Damien; Chasset, Sophie Retrovirology, Vol. 10, Issue 1 https://doi.org/10.1186/1742-4690-10-144	journal	January 2013
The Conserved Set of Host Proteins Incorporated into HIV-1 Virions Suggests a Common Egress Pathway in Multiple Cell Types Linde, Michael E.; Colquhoun, David R.; Ubaida Mohien, Ceereena Journal of Proteome Research, Vol. 12, Issue 5 https://doi.org/10.1021/pr300918r	journal	March 2013
Solution Structure of the DNA Binding Domain of HIV-1 Integrase Lodi, Patricia J.; Ernst, James A.; Kuszewski, John Biochemistry, Vol. 34, Issue 31 https://doi.org/10.1021/bi00031a002	journal	August 1995
Genetic Analyses of Conserved Residues in the Carboxyl-Terminal Domain of Human Immunodeficiency Virus Type 1 Integrase Lu, Richard; Ghory, Hina Z.; Engelman, Alan Journal of Virology, Vol. 79, Issue 16 https://doi.org/10.1128/JVI.79.16.10356-10368.2005	journal	August 2005
Structure-Based Mutational Analysis of the C-Terminal DNA-Binding Domain of Human Immunodeficiency Virus Type 1 Integrase: Critical Residues for Protein Oligomerization and DNA Binding Lutzke, Ramon A. Puras; Plasterk, Ronald H. A. Journal of Virology, Vol. 72, Issue 6 https://doi.org/10.1128/JVI.72.6.4841-4848.1998	journal	June 1998
Allosteric HIV-1 Integrase Inhibitors Lead to Premature Degradation of the Viral RNA Genome and Integrase in Target Cells Madison, Michaela K.; Lawson, Dana Q.; Elliott, Jennifer Journal of Virology, Vol. 91, Issue 17 https://doi.org/10.1128/JVI.00821-17	journal	September 2017
Evolving factor analysis for the resolution of overlapping chromatographic peaks Maeder, Marcel. Analytical Chemistry, Vol. 59, Issue 3 https://doi.org/10.1021/ac00130a035	journal	February 1987
The mechanism of retroviral integration from X-ray structures of its key intermediates Maertens, Goedele N.; Hare, Stephen; Cherepanov, Peter Nature, Vol. 468, Issue 7321 https://doi.org/10.1038/nature09517	journal	November 2010
Structural basis for retroviral integration into nucleosomes Maskell, Daniel P.; Renault, Ludovic; Serrao, Erik Nature, Vol. 523, Issue 7560 https://doi.org/10.1038/nature14495	journal	June 2015
Human H4 tail stimulates HIV-1 integration through binding to the carboxy-terminal domain of integrase Mauro, Eric; Lesbats, Paul; Lapaillerie, Delphine Nucleic Acids Research, Vol. 47, Issue 7 https://doi.org/10.1093/nar/gkz091	journal	February 2019
Domain Movements upon Activation of Phenylalanine Hydroxylase Characterized by Crystallography and Chromatography-Coupled Small-Angle X-ray Scattering Meisburger, Steve P.; Taylor, Alexander B.; Khan, Crystal A. Journal of the American Chemical Society, Vol. 138, Issue 20 https://doi.org/10.1021/jacs.6b01563	journal	May 2016
Structural Analysis of a Mutant of the HIV-1 Integrase Zinc Finger Domain That Forms a Single Conformation Nomura, Yusuke; Masuda, Takao; Kawai, Gota The Journal of Biochemistry, Vol. 139, Issue 4 https://doi.org/10.1093/jb/mvj085	journal	April 2006
Functional Interactions of Human Immunodeficiency Virus Type 1 Integrase with Human and Yeast HSP60 Parissi, Vincent; Calmels, Christina; De Soultrait, Vaea Richard Journal of Virology, Vol. 75, Issue 23 https://doi.org/10.1128/JVI.75.23.11344-11353.2001	journal	December 2001
Cryo-EM structures and atomic model of the HIV-1 strand transfer complex intasome Passos, Dario Oliveira; Li, Min; Yang, Renbin Science, Vol. 355, Issue 6320 https://doi.org/10.1126/science.aah5163	journal	January 2017
Scalable molecular dynamics with NAMD Phillips, James C.; Braun, Rosemary; Wang, Wei Journal of Computational Chemistry, Vol. 26, Issue 16, p. 1781-1802 https://doi.org/10.1002/jcc.20289	journal	January 2005
Effects of Macromolecular Crowding on the Inhibition of Virus Assembly and Virus-Cell Receptor Recognition Rincón, Verónica; Bocanegra, Rebeca; Rodríguez-Huete, Alicia Biophysical Journal, Vol. 100, Issue 3 https://doi.org/10.1016/j.bpj.2010.12.3714	journal	February 2011
HIV-1 Integration in the Human Genome Favors Active Genes and Local Hotspots Schröder, Astrid R. W.; Shinn, Paul; Chen, Huaming Cell, Vol. 110, Issue 4 https://doi.org/10.1016/S0092-8674(02)00864-4	journal	August 2002
Size-Distribution Analysis of Macromolecules by Sedimentation Velocity Ultracentrifugation and Lamm Equation Modeling Schuck, Peter Biophysical Journal, Vol. 78, Issue 3 https://doi.org/10.1016/S0006-3495(00)76713-0	journal	March 2000
GNOM – a program package for small-angle scattering data processing Semenyuk, A. V.; Svergun, D. I. Journal of Applied Crystallography, Vol. 24, Issue 5 https://doi.org/10.1107/S002188989100081X	journal	October 1991
A New Class of Multimerization Selective Inhibitors of HIV-1 Integrase Sharma, Amit; Slaughter, Alison; Jena, Nivedita PLoS Pathogens, Vol. 10, Issue 5 https://doi.org/10.1371/journal.ppat.1004171	journal	May 2014
LEDGF/p75 functions downstream from preintegration complex formation to effect gene-specific HIV-1 integration Shun, M. -C.; Raghavendra, N. K.; Vandegraaff, N. Genes & Development, Vol. 21, Issue 14 https://doi.org/10.1101/gad.1565107	journal	July 2007
CRYSOL – a Program to Evaluate X-ray Solution Scattering of Biological Macromolecules from Atomic Coordinates Svergun, D.; Barberato, C.; Koch, M. H. J. Journal of Applied Crystallography, Vol. 28, Issue 6 https://doi.org/10.1107/S0021889895007047	journal	December 1995
Small-angle scattering by fractal systems Teixeira, J. Journal of Applied Crystallography, Vol. 21, Issue 6, p. 781-785 https://doi.org/10.1107/S0021889888000263	journal	December 1988
Interaction between Reverse Transcriptase and Integrase Is Required for Reverse Transcription during HIV-1 Replication Tekeste, Shewit S.; Wilkinson, Thomas A.; Weiner, Ethan M. Journal of Virology, Vol. 89, Issue 23 https://doi.org/10.1128/JVI.01471-15	journal	September 2015
Sedimentation equilibrium analysis of protein interactions with global implicit mass conservation constraints and systematic noise decomposition Vistica, Jennifer; Dam, Julie; Balbo, Andrea Analytical Biochemistry, Vol. 326, Issue 2 https://doi.org/10.1016/j.ab.2003.12.014	journal	March 2004
Structure of a two-domain fragment of HIV-1 integrase: implications for domain organization in the intact protein Wang, J. -Y. The EMBO Journal, Vol. 20, Issue 24 https://doi.org/10.1093/emboj/20.24.7333	journal	December 2001
Two-Stage PCR Protocol Allowing Introduction of Multiple Mutations, Deletions and Insertions Using QuikChange ^TM Site-Directed Mutagenesis Wang, Wenyan; Malcolm, Bruce A. BioTechniques, Vol. 26, Issue 4 https://doi.org/10.2144/99264st03	journal	April 1999
MULCh : modules for the analysis of small-angle neutron contrast variation data from biomolecular assemblies Whitten, Andrew E.; Cai, Shuzhi; Trewhella, Jill Journal of Applied Crystallography, Vol. 41, Issue 1 https://doi.org/10.1107/S0021889807055136	journal	January 2008
Retroviral integration into nucleosomes through DNA looping and sliding along the histone octamer Wilson, Marcus D.; Renault, Ludovic; Maskell, Daniel P. Nature Communications, Vol. 10, Issue 1 https://doi.org/10.1038/s41467-019-12007-w	journal	September 2019
Crystal structure of the Rous sarcoma virus intasome Yin, Zhiqi; Shi, Ke; Banerjee, Surajit Nature, Vol. 530, Issue 7590 https://doi.org/10.1038/nature16950	journal	February 2016
Selective Histonedeacetylase Inhibitor M344 Intervenes in HIV-1 Latency through Increasing Histone Acetylation and Activation of NF-kappaB Ying, Hao; Zhang, Yuhao; Zhou, Xin PLoS ONE, Vol. 7, Issue 11 https://doi.org/10.1371/journal.pone.0048832	journal	November 2012

Similar Records

Diacylglycerol kinase β induces filopodium formation via its C1, catalytic and carboxy-terminal domains and interacts with the Rac1-GTPase-activating protein, β2-chimaerin

Journal Article · 2018 · Biochemical and Biophysical Research Communications · OSTI ID:1783997

Maeda, Yuki; Shibata, Kosuke; Akiyama, Rino; +6 more

Discovery and Optimization of Novel Pyrazolopyrimidines as Potent and Orally Bioavailable Allosteric HIV-1 Integrase Inhibitors

Journal Article · 2020 · Journal of Medicinal Chemistry · OSTI ID:1783997

Li, Guo; Meanwell, Nicholas A.; Krystal, Mark R.; +17 more

5,6,7,8-Tetrahydro-1,6-naphthyridine Derivatives as Potent HIV-1-Integrase-Allosteric-Site Inhibitors

Journal Article · 2019 · J. Med. Chem. · OSTI ID:1783997

Peese, Kevin M.; Allard, Christopher W.; Connolly, Timothy; +18 more

Related Subjects

99 GENERAL AND MISCELLANEOUS

Title: Allosteric HIV Integrase Inhibitors Promote Formation of Inactive Branched Polymers via Homomeric Carboxy-Terminal Domain Interactions

Citation Formats

References (88)

Similar Records

Related Subjects