Intracellular pathways for lignin catabolism in white-rot fungi

Del Cerro, Carlos; Erickson, Erika; Dong, Tao; Wong, Allison R.; Eder, Elizabeth; Purvine, Samuel O.; Mitchell, Hugh D.; Weitz, Karl K.; Markillie, Lye Meng; Burnet, Meagan C.; Hoyt, David W.; Chu, Rosalie K.; Cheng, Jan-Feng; Ramirez, Kelsey; Katahira, Rui; Xiong, Wei; Himmel, Michael E.; Subramanian, Venkataramanan; Linger, Jeffrey; Salvachua, Davinia

doi:10.1073/pnas.2017381118

Title: Intracellular pathways for lignin catabolism in white-rot fungi

Journal Article · Tue Mar 02 00:00:00 EST 2021 · Proceedings of the National Academy of Sciences (PNAS).

DOI:https://doi.org/10.1073/pnas.2017381118· OSTI ID:1778836

Del Cerro, Carlos ^[1]; Erickson, Erika ^[1]; Dong, Tao ^[1]; Wong, Allison R. ^[2]; Eder, Elizabeth ^[2];

^[2]; Mitchell, Hugh D. ^[2]; Weitz, Karl K. ^[2]; Markillie, Lye Meng ^[2]; Burnet, Meagan C. ^[2];

^[2]; Chu, Rosalie K. ^[2]; Cheng, Jan-Feng ^[3]; Ramirez, Kelsey ^[1]; Katahira, Rui ^[1]; Xiong, Wei ^[1]; Himmel, Michael E. ^[4]; Subramanian, Venkataramanan ^[1]; Linger, Jeffrey ^[1]; Salvachua, Davinia ^[1]

National Renewable Energy Laboratory
BATTELLE (PACIFIC NW LAB)
Department of Energy Joint Genome Institute
NREL

White-rot fungi, the most efficient organisms at breaking down lignin from wood in Nature, utilize lignin degradation products as a carbon source. 15 Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at degrading lignin in Nature. While lignin depolymerization by WRF has been exhaustively studied, the possibility that WRF are able to utilize lignin as a carbon source is still a matter of controversy. Here we employ 13C-labeling and systems biology approaches to demonstrate that two WRF, Trametes versicolor and Gelatoporia subvermispora, funnel lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways. These results provide insights into global carbon cycling in 20 soil ecosystems, and furthermore establishes a foundation for employing WRF in simultaneous lignin depolymerization and bioconversion to bioproducts – a key step towards enabling a sustainable bioeconomy.

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Research Organization:: Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1778836

Report Number(s):: PNNL-SA-155713

Journal Information:: Proceedings of the National Academy of Sciences (PNAS)., Vol. 118, Issue 9

Country of Publication:: United States

Language:: English

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