White-rot fungi, the most efficient organisms at breaking down lignin from wood in Nature, utilize lignin degradation products as a carbon source. 15 Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at degrading lignin in Nature. While lignin depolymerization by WRF has been exhaustively studied, the possibility that WRF are able to utilize lignin as a carbon source is still a matter of controversy. Here we employ 13C-labeling and systems biology approaches to demonstrate that two WRF, Trametes versicolor and Gelatoporia subvermispora, funnel lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways. These results provide insights into global carbon cycling in 20 soil ecosystems, and furthermore establishes a foundation for employing WRF in simultaneous lignin depolymerization and bioconversion to bioproducts – a key step towards enabling a sustainable bioeconomy.
Del Cerro, Carlos, et al. "Intracellular pathways for lignin catabolism in white-rot fungi." Proceedings of the National Academy of Sciences (PNAS)., vol. 118, no. 9, Mar. 2021. https://doi.org/10.1073/pnas.2017381118
Del Cerro, Carlos, Erickson, Erika, Dong, Tao, Wong, Allison R., Eder, Elizabeth, Purvine, Samuel O., Mitchell, Hugh D., Weitz, Karl K., Markillie, Lye Meng, Burnet, Meagan C., Hoyt, David W., Chu, Rosalie K., Cheng, Jan-Feng, Ramirez, Kelsey, Katahira, Rui, Xiong, Wei, Himmel, Michael E., Subramanian, Venkataramanan, Linger, Jeffrey, & Salvachua, Davinia (2021). Intracellular pathways for lignin catabolism in white-rot fungi. Proceedings of the National Academy of Sciences (PNAS)., 118(9). https://doi.org/10.1073/pnas.2017381118
Del Cerro, Carlos, Erickson, Erika, Dong, Tao, et al., "Intracellular pathways for lignin catabolism in white-rot fungi," Proceedings of the National Academy of Sciences (PNAS). 118, no. 9 (2021), https://doi.org/10.1073/pnas.2017381118
@article{osti_1778836,
author = {Del Cerro, Carlos and Erickson, Erika and Dong, Tao and Wong, Allison R. and Eder, Elizabeth and Purvine, Samuel O. and Mitchell, Hugh D. and Weitz, Karl K. and Markillie, Lye Meng and Burnet, Meagan C. and others},
title = {Intracellular pathways for lignin catabolism in white-rot fungi},
annote = {White-rot fungi, the most efficient organisms at breaking down lignin from wood in Nature, utilize lignin degradation products as a carbon source. 15 Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at degrading lignin in Nature. While lignin depolymerization by WRF has been exhaustively studied, the possibility that WRF are able to utilize lignin as a carbon source is still a matter of controversy. Here we employ 13C-labeling and systems biology approaches to demonstrate that two WRF, Trametes versicolor and Gelatoporia subvermispora, funnel lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways. These results provide insights into global carbon cycling in 20 soil ecosystems, and furthermore establishes a foundation for employing WRF in simultaneous lignin depolymerization and bioconversion to bioproducts – a key step towards enabling a sustainable bioeconomy.},
doi = {10.1073/pnas.2017381118},
url = {https://www.osti.gov/biblio/1778836},
journal = {Proceedings of the National Academy of Sciences (PNAS).},
number = {9},
volume = {118},
place = {United States},
year = {2021},
month = {03}}
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1778836
Report Number(s):
PNNL-SA-155713
Journal Information:
Proceedings of the National Academy of Sciences (PNAS)., Journal Name: Proceedings of the National Academy of Sciences (PNAS). Journal Issue: 9 Vol. 118