Intracellular pathways for lignin catabolism in white-rot fungi

del Cerro, Carlos; Erickson, Erika; Dong, Tao; Wong, Allison R.; Eder, Elizabeth K.; Purvine, Samuel O.; Mitchell, Hugh D.; Weitz, Karl K.; Markillie, Lye Meng; Burnet, Meagan C.; Hoyt, David W.; Chu, Rosalie K.; Cheng, Jan-Fang; Ramirez, Kelsey J.; Katahira, Rui; Xiong, Wei; Himmel, Michael E.; Subramanian, Venkataramanan; Linger, Jeffrey G.; Salvachúa, Davinia

doi:10.1073/pnas.2017381118

Title: Intracellular pathways for lignin catabolism in white-rot fungi

Journal Article · Tue Feb 23 00:00:00 EST 2021 · Proceedings of the National Academy of Sciences of the United States of America

DOI:https://doi.org/10.1073/pnas.2017381118· OSTI ID:1766898

;

; Weitz, Karl K.;

; Burnet, Meagan C.;

;

; Cheng, Jan-Fang;

; Katahira, Rui; Xiong, Wei; Himmel, Michael E.; Subramanian, Venkataramanan; Linger, Jeffrey G.;

Lignin is a biopolymer found in plant cell walls that accounts for 30% of the organic carbon in the biosphere. White-rot fungi (WRF) are considered the most efficient organisms at degrading lignin in nature. While lignin depolymerization by WRF has been extensively studied, the possibility that WRF are able to utilize lignin as a carbon source is still a matter of controversy. Here, we employ ¹³C-isotope labeling, systems biology approaches, and in vitro enzyme assays to demonstrate that two WRF, Trametes versicolor and Gelatoporia subvermispora, funnel carbon from lignin-derived aromatic compounds into central carbon metabolism via intracellular catabolic pathways. These results provide insights into global carbon cycling in soil ecosystems and furthermore establish a foundation for employing WRF in simultaneous lignin depolymerization and bioconversion to bioproducts—a key step toward enabling a sustainable bioeconomy.

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Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Laboratory Directed Research and Development (LDRD) Program; USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office; USDOE Office of Science (SC), Biological and Environmental Research (BER)

Grant/Contract Number:: AC36-08GO28308; AC02-05CH11231

OSTI ID:: 1766898

Alternate ID(s):: OSTI ID: 1770922

Report Number(s):: NREL/JA-2800-77735; e2017381118

Journal Information:: Proceedings of the National Academy of Sciences of the United States of America, Journal Name: Proceedings of the National Academy of Sciences of the United States of America Vol. 118 Journal Issue: 9; ISSN 0027-8424

Publisher:: National Academy of SciencesCopyright Statement

Country of Publication:: United States

Language:: English

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59 BASIC BIOLOGICAL SCIENCES
lignin
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aromatic compounds
depolymerization
carbon cycling
metabolism
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