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The Polyketide Synthase Gene pks4 of Trichoderma reesei Provides Pigmentation and Stress Resistance

Journal Article · · Eukaryotic Cell
DOI:https://doi.org/10.1128/EC.00103-13· OSTI ID:1776806
 [1];  [2];  [3];  [3];  [2]
  1. Vienna University of Technology
  2. BATTELLE (PACIFIC NW LAB)
  3. Technische Universitat Wien
+ Show Author Affiliations
Species of the fungal genus Trichoderma (Hypocreales, Ascomycota) are well-known for their production of various secondary metabolites. Nonribosomal peptides and polyketides represent a major portion of these products. In a recent phylogenomic investigation of Trichoderma polyketide synthase (PKS)-encoding genes, the pks4 from T. reesei was shown to be an orthologue of pigment-forming PKSs involved in synthesis of aurofusarin and bikaverin in Fusarium spp. In this study, we show that deletion of this gene in T. reesei results in loss of green conidial pigmentation and in pigmentation alteration of teleomorph structures. It also has an impact on conidial cell wall stability and the antagonistic abilities of T. reesei against other fungi, including formation of inhibitory metabolites. In addition, deletion of pks4 significantly influences the expression of other PKS-encoding genes of T. reesei. To our knowledge, this is the first indication that a low-molecular-weight pigment-forming PKS is involved in defense, mechanical stability, and stress resistance in fungi.
Research Organization:
Pacific Northwest National Laboratory (PNNL), Richland, WA (United States)
Sponsoring Organization:
USDOE
DOE Contract Number:
AC05-76RL01830
OSTI ID:
1776806
Report Number(s):
PNNL-SA-100641
Journal Information:
Eukaryotic Cell, Journal Name: Eukaryotic Cell Journal Issue: 11 Vol. 12
Country of Publication:
United States
Language:
English

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