FRET sensor-based quantification of intracellular trehalose in mammalian cells

Kikuta, Shingo; Hou, Bi-Huei; Sato, Ryoichi; Frommer, Wolf B.; Kikawada, Takahiro

doi:10.1080/09168451.2015.1069699

FRET sensor-based quantification of intracellular trehalose in mammalian cells

Journal Article · Sat Jan 02 00:00:00 EST 2016 · Bioscience, Biotechnology, and Biochemistry

DOI:https://doi.org/10.1080/09168451.2015.1069699· OSTI ID:1770459

Kikuta, Shingo ^[1]; Hou, Bi-Huei ^[2]; Sato, Ryoichi ^[3]; Frommer, Wolf B. ^[2]; Kikawada, Takahiro ^[4]

Insect Mimetics Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Japan, Graduate School of Bio-Applications & Systems Engineering (BASE), Tokyo University of Agriculture and Technology, Tokyo, Japan
Department of Plant Biology, Carnegie Institution for Science, Stanford, CA, USA
Graduate School of Bio-Applications & Systems Engineering (BASE), Tokyo University of Agriculture and Technology, Tokyo, Japan
Insect Mimetics Research Unit, National Institute of Agrobiological Sciences, Tsukuba, Japan

Abstract

Trehalose acts as a stress protectant and an autophagy inducer in mammalian cells. The molecular mechanisms of action remain obscure because intracellular trehalose at micromolar level is difficult to quantitate. Here, we show a novel trehalose monitoring technology based on FRET. FLIPsuc90μ∆1Venus sensor expressed in mammalian cells enables to quickly and non-destructively detect an infinitesimal amount of intracellular trehalose.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: FG02-04ER15542

OSTI ID:: 1770459

Journal Information:: Bioscience, Biotechnology, and Biochemistry, Journal Name: Bioscience, Biotechnology, and Biochemistry Journal Issue: 1 Vol. 80; ISSN 0916-8451

Publisher:: Informa UK LimitedCopyright Statement

Country of Publication:: Japan

Language:: English

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