Activity-based protein profiling of chitin catabolism

Zegeye, Elias; Sadler, Natalie C.; Lomas, Gerard X.; Attah, Isaac K.; Jansson, Janet K.; Hofmockel, Kirsten S.; Anderton, Christopher R.; Wright, Aaron T.

doi:10.1002/cbic.202000616

Title: Activity-based protein profiling of chitin catabolism

Journal Article · Mon Feb 15 00:00:00 EST 2021 · Chembiochem

DOI:https://doi.org/10.1002/cbic.202000616· OSTI ID:1770121

Zegeye, Elias ^[1]; Sadler, Natalie C. ^[2]; Lomas, Gerard X. ^[2]; Attah, Isaac K. ^[2]; Jansson, Janet K. ^[2];

^[2]; Anderton, Christopher R. ^[2];

^[2]

WASHINGTON STATE UNIV
BATTELLE (PACIFIC NW LAB)

Microbial catabolism of chitin, a highly abundant component of polymeric organic matter worldwide, is a fundamental cog in the carbon and nitrogen cycles that govern CO2 emissions, nutrient dynamics in aquatic systems, and soil vitality. Despite this critical biogeochemical function, we have a poor understanding of the synergy between various microbial chitinases and key accessory enzymes involved in chitin catabolism. We designed and synthesized activity-based probes (ABPs) for profiling active chitinolytic enzymes by modifying the chitin derivatives N-acetyl glucosamine and chitotriose. The ABPs were used to determine the active complement of chitinolytic enzymes produced over time by the soil bacterium Cellvibrio japonicus treated with various C substrates. This approach allowed us to demonstrate the utility of these ABPs in determining the intricate synergy between chitinases and other glycoside hydrolases that microbes employ to catabolize chitin. The strategy can be used to gain molecular level insights that can be used to better understand microbial roles in soil C and N cycling in the face of a changing climate.

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Research Organization:: Pacific Northwest National Lab. (PNNL), Richland, WA (United States)

Sponsoring Organization:: USDOE

DOE Contract Number:: AC05-76RL01830

OSTI ID:: 1770121

Report Number(s):: PNNL-SA-156004

Journal Information:: Chembiochem, Vol. 22, Issue 4

Country of Publication:: United States

Language:: English

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