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3D diffractive imaging of nanoparticle ensembles using an x-ray laser

Journal Article · · Optica
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  1. Max Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. Hamburg (Germany). Hamburg Center for Ultrafast Imaging
  2. Max Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany); Univ. Hamburg (Germany). Hamburg Center for Ultrafast Imaging; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  3. European X-ray Free-Electron Laser (XFEL), Schenefeld (Germany)
  4. National Univ. of Singapore (Singapore). Center for BioImaging Sciences
  5. Hamburg Center for Ultrafast Imaging; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  6. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  7. Uppsala Univ., Uppsala (Sweden)
  8. SLAC National Accelerator Lab., Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)
  9. Arizona State Univ., Tempe, AZ (United States)
  10. Univ. Hamburg (Germany). Hamburg Center for Ultrafast Imaging, and Inst. of Physical Chemistry
  11. Univ. Hamburg (Germany). Hamburg Center for Ultrafast Imaging; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  12. Univ. of Melbourne, VIC (Australia)
  13. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Univ. Hamburg (Germany)
  14. European XFEL, Schenefeld (Germany)
  15. Univ. Hamburg (Germany). Inst. of Physical Chemistry
  16. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; European X-ray Free-Electron Laser (XFEL), Schenefeld (Germany)
  17. Max Planck Inst. for the Structure and Dynamics of Matter, Hamburg (Germany); Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science
  18. Uppsala Univ. (Sweden); Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States). National Energy Research Scientific Computing Center (NERSC)
  19. Univ. Hamburg (Germany). Hamburg Center for Ultrafast Imaging; Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany). Center for Free-Electron Laser Science; Radboud Univ., Nijmegen (Netherlands). Inst. for Molecules and Materials
  20. European XFEL, Schenefeld (Germany); La Trobe Univ., Melbourne, VIC (Australia). La Trobe Inst. for Molecular Science
+ Show Author Affiliations
Single particle imaging at x-ray free electron lasers (XFELs) has the potential to determine the structure and dynamics of single biomolecules at room temperature. Two major hurdles have prevented this potential from being reached, namely, the collection of sufficient high-quality diffraction patterns and robust computational purification to overcome structural heterogeneity. We report the breaking of both of these barriers using gold nanoparticle test samples, recording around 10 million diffraction patterns at the European XFEL and structurally and orientationally sorting the patterns to obtain better than 3-nm-resolution 3D reconstructions for each of four samples. With these new developments, integrating advancements in x-ray sources, fast-framing detectors, efficient sample delivery, and data analysis algorithms, we illuminate the path towards sub-nanometer biomolecular imaging. The methods developed here can also be extended to characterize ensembles that are inherently diverse to obtain their full structural landscape.
Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
Carl Tryggers Foundation for Scientific Research; European Research Council (ERC); German Research Foundation (DFG); Helmholtz Association; Röntgen-Ångström Cluster; Swedish Research Council (SRC); USDOE Office of Science (SC), Basic Energy Sciences (BES)
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1768221
Journal Information:
Optica, Journal Name: Optica Journal Issue: 1 Vol. 8; ISSN 2334-2536
Publisher:
Optical Society of AmericaCopyright Statement
Country of Publication:
United States
Language:
English

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3D diffractive imaging of nanoparticle ensembles using an x-ray laser dataset January 2024

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