Atypical chemoreceptor arrays accommodate high membrane curvature

Muok, Alise R.; Ortega, Davi R.; Kurniyati, Kurni; Yang, Wen; Maschmann, Zachary A.; Sidi Mabrouk, Adam; Li, Chunhao; Crane, Brian R.; Briegel, Ariane

doi:10.1038/s41467-020-19628-6

Title: Atypical chemoreceptor arrays accommodate high membrane curvature

Journal Article · Fri Nov 13 00:00:00 EST 2020 · Nature Communications

DOI:https://doi.org/10.1038/s41467-020-19628-6· OSTI ID:1767227

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Leiden Univ. (Netherlands)
California Institute of Technology (CalTech), Pasadena, CA (United States)
Virginia Commonwealth Univ., Richmond, VA (United States)
Cornell Univ., Ithaca, NY (United States)

The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended array. Here, we report an alternative symmetry (P2) of the chemotaxis apparatus that emerges from a strict linear organization of the histidine kinase CheA in Treponema denticola cells, which possesses arrays with the highest native curvature investigated thus far. Using cryo-ET, we reveal that Td chemoreceptor arrays assume an unusual arrangement of the supra-molecular protein assembly that has likely evolved to accommodate the high membrane curvature. The arrays have several atypical features, such as an extended dimerization domain of CheA and a variant CheW-CheR-like fusion protein that is critical for maintaining an ordered chemosensory apparatus. Furthermore, the previously characterized Td oxygen sensor ODP influences CheA ordering. These results suggest a greater diversity of the chemotaxis signaling system than previously thought.

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Research Organization:: Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)

Sponsoring Organization:: USDOE Office of Science (SC); National Institutes of Health (NIH); Dutch Research Council (NWO); European Union (EU); National Institute of General Medical Sciences (NIGMS)

Grant/Contract Number:: R35GM122535; R01AI078958; R01DE023080; P30 GM124165; S10 RR029205

OSTI ID:: 1767227

Alternate ID(s):: OSTI ID: 1770424

Journal Information:: Nature Communications, Vol. 11, Issue 1; ISSN 2041-1723

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: ENGLISH

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