Evolutionary genomics suggests that CheV is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex

Ortega, Davi R.; Zhulin, Igor B.; Punta, Marco

doi:10.1371/journal.pcbi.1004723

Evolutionary genomics suggests that CheV is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex

Journal Article · Thu Feb 04 04:00:00 EST 2016 · PLoS Computational Biology (Online)

DOI:https://doi.org/10.1371/journal.pcbi.1004723· OSTI ID:1327747

Ortega, Davi R. ^[1]; Zhulin, Igor B. ^[2]; Punta, Marco ^[3]

California Inst. of Technology (CalTech), Pasadena, CA (United States); Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States); Univ. of Tennessee, Knoxville, TN (United States)
Pierre and Marie Curie Univ. (UPMC), Paris (France)

Escherichia coli and Salmonella enterica are models for many experiments in molecular biology including chemotaxis, and most of the results obtained with one organism have been generalized to another. While most components of the chemotaxis pathway are strongly conserved between the two species, Salmonella genomes contain some chemoreceptors and an additional protein, CheV, that are not found in E. coli. The role of CheV was examined in distantly related species Bacillus subtilis and Helicobacter pylori, but its role in bacterial chemotaxis is still not well understood. We tested a hypothesis that in enterobacteria CheV functions as an additional adaptor linking the CheA kinase to certain types of chemoreceptors that cannot be effectively accommodated by the universal adaptor CheW. Phylogenetic profiling, genomic context and comparative protein sequence analyses suggested that CheV interacts with specific domains of CheA and chemoreceptors from an orthologous group exemplified by the Salmonella McpC protein. Structural consideration of the conservation patterns suggests that CheV and CheW share the same binding spot on the chemoreceptor structure, but have some affinity bias towards chemoreceptors from different orthologous groups. Finally, published experimental results and data newly obtained via comparative genomics support the idea that CheV functions as a "phosphate sink" possibly to off-set the over-stimulation of the kinase by certain types of chemoreceptors. Altogether, our results strongly suggest that CheV is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex.

View Accepted Manuscript (DOE)

Research Organization:: Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)

Sponsoring Organization:: USDOE

Grant/Contract Number:: AC05-00OR22725

OSTI ID:: 1327747

Journal Information:: PLoS Computational Biology (Online), Journal Name: PLoS Computational Biology (Online) Journal Issue: 2 Vol. 12; ISSN 1553-7358

Publisher:: Public Library of ScienceCopyright Statement

Country of Publication:: United States

Language:: English

References (77)

Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis Harayama, Shigeaki; Palva, E. Tapio; Hazelbauer, Gerald L. Molecular and General Genetics MGG, Vol. 171, Issue 2 https://doi.org/10.1007/bf00270005	journal	January 1979
Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli Li, You; Hu, Yunfei; Fu, Wenyu Biochemical and Biophysical Research Communications, Vol. 360, Issue 4 https://doi.org/10.1016/j.bbrc.2007.06.146	journal	September 2007
An Infection-Relevant Transcriptomic Compendium for Salmonella enterica Serovar Typhimurium Kröger, Carsten; Colgan, Aoife; Srikumar, Shabarinath Cell Host & Microbe, Vol. 14, Issue 6 https://doi.org/10.1016/j.chom.2013.11.010	journal	December 2013
Direct Evidence for Coupling between Bacterial Chemoreceptors Vaknin, Ady; Berg, Howard C. Journal of Molecular Biology, Vol. 382, Issue 3 https://doi.org/10.1016/j.jmb.2008.07.026	journal	October 2008
Protein Footprinting in a Complex Milieu: Identifying the Interaction Surfaces of the Chemotaxis Adaptor Protein CheW Underbakke, Eric S.; Zhu, Yimin; Kiessling, Laura L. Journal of Molecular Biology, Vol. 409, Issue 4 https://doi.org/10.1016/j.jmb.2011.03.040	journal	June 2011
Bacterial chemoreceptors: high-performance signaling in networked arrays Hazelbauer, Gerald L.; Falke, Joseph J.; Parkinson, John S. Trends in Biochemical Sciences, Vol. 33, Issue 1 https://doi.org/10.1016/j.tibs.2007.09.014	journal	January 2008
CheV: CheW-like coupling proteins at the core of the chemotaxis signaling network Alexander, Roger P.; Lowenthal, Andrew C.; Harshey, Rasika M. Trends in Microbiology, Vol. 18, Issue 11 https://doi.org/10.1016/j.tim.2010.07.004	journal	November 2010
Solution Structure of a Complex of the Histidine Autokinase CheA with Its Substrate CheY Mo, Guoya; Zhou, Hongjun; Kawamura, Tetsuya Biochemistry, Vol. 51, Issue 18 https://doi.org/10.1021/bi300147m	journal	April 2012
The 3.2 Å Resolution Structure of a Receptor:CheA:CheW Signaling Complex Defines Overlapping Binding Sites and Key Residue Interactions within Bacterial Chemosensory Arrays Li, Xiaoxiao; Fleetwood, Aaron D.; Bayas, Camille Biochemistry, Vol. 52, Issue 22 https://doi.org/10.1021/bi400383e	journal	May 2013
New Insights into Bacterial Chemoreceptor Array Structure and Assembly from Electron Cryotomography Briegel, Ariane; Wong, Margaret L.; Hodges, Heather L. Biochemistry, Vol. 53, Issue 10 https://doi.org/10.1021/bi5000614	journal	March 2014
Phosphotransfer between CheA, CheY1, and CheY2 in the Chemotaxis Signal Transduction Chain of Rhizobium meliloti ^† Sourjik, Victor; Schmitt, Rüdiger Biochemistry, Vol. 37, Issue 8 https://doi.org/10.1021/bi972330a	journal	February 1998
Four-helical-bundle structure of the cytoplasmic domain of a serine chemotaxis receptor Kim, Kyeong Kyu; Yokota, Hisao; Kim, Sung-Hou Nature, Vol. 400, Issue 6746 https://doi.org/10.1038/23512	journal	August 1999
Model is as model does Manson, Michael D.; Cantwell, Brian J. Nature Cell Biology, Vol. 2, Issue 11 https://doi.org/10.1038/35041118	journal	November 2000
Making sense of it all: bacterial chemotaxis Wadhams, George H.; Armitage, Judith P. Nature Reviews Molecular Cell Biology, Vol. 5, Issue 12 https://doi.org/10.1038/nrm1524	journal	December 2004
Ancient chemoreceptors retain their flexibility Parkinson, John S. Proceedings of the National Academy of Sciences, Vol. 104, Issue 8 https://doi.org/10.1073/pnas.0700278104	journal	February 2007
Universal architecture of bacterial chemoreceptor arrays Briegel, A.; Ortega, D. R.; Tocheva, E. I. Proceedings of the National Academy of Sciences, Vol. 106, Issue 40 https://doi.org/10.1073/pnas.0905181106	journal	September 2009
Bacterial chemoreceptor arrays are hexagonally packed trimers of receptor dimers networked by rings of kinase and coupling proteins Briegel, A.; Li, X.; Bilwes, A. M. Proceedings of the National Academy of Sciences, Vol. 109, Issue 10 https://doi.org/10.1073/pnas.1115719109	journal	February 2012
Cooperativity between bacterial chemotaxis receptors Falke, Joseph J. Proceedings of the National Academy of Sciences, Vol. 99, Issue 10 https://doi.org/10.1073/pnas.112214199	journal	May 2002
Sensory transduction in Escherichia coli: two complementary pathways of information processing that involve methylated proteins. Springer, M. S.; Goy, M. F.; Adler, J. Proceedings of the National Academy of Sciences, Vol. 74, Issue 8 https://doi.org/10.1073/pnas.74.8.3312	journal	August 1977
Cloning and characterization of the Salmonella typhimurium-specific chemoreceptor Tcp for taxis to citrate and from phenol. Yamamoto, K.; Imae, Y. Proceedings of the National Academy of Sciences, Vol. 90, Issue 1 https://doi.org/10.1073/pnas.90.1.217	journal	January 1993
Assigning protein functions by comparative genome analysis: Protein phylogenetic profiles Pellegrini, M.; Marcotte, E. M.; Thompson, M. J. Proceedings of the National Academy of Sciences, Vol. 96, Issue 8 https://doi.org/10.1073/pnas.96.8.4285	journal	April 1999
Phosphorylation of the Response Regulator CheV Is Required for Adaptation to Attractants during Bacillus subtilis Chemotaxis Karatan, Ece; Saulmon, Michael M.; Bunn, Michael W. Journal of Biological Chemistry, Vol. 276, Issue 47 https://doi.org/10.1074/jbc.m104955200	journal	September 2001
A Simple, Fast, and Accurate Algorithm to Estimate Large Phylogenies by Maximum Likelihood Guindon, Stéphane; Gascuel, Olivier Systematic Biology, Vol. 52, Issue 5 https://doi.org/10.1080/10635150390235520	journal	October 2003
The MiST2 database: a comprehensive genomics resource on microbial signal transduction Ulrich, Luke E.; Zhulin, Igor B. Nucleic Acids Research, Vol. 38, Issue suppl_1 https://doi.org/10.1093/nar/gkp940	journal	November 2009
Chemotaxis in the human gastric pathogen Helicobacter pylori: different roles for CheW and the three CheV paralogues, and evidence for CheV2 phosphorylation Pittman, Marc S.; Goodwin, Matthew; Kelly, David J. Microbiology, Vol. 147, Issue 9 https://doi.org/10.1099/00221287-147-9-2493	journal	September 2001
A fixed-time diffusion analysis method determines that the three cheV genes of Helicobacter pylori differentially affect motility Lowenthal, Andrew C.; Simon, Christopher; Fair, Amber S. Microbiology, Vol. 155, Issue 4 https://doi.org/10.1099/mic.0.021857-0	journal	April 2009
Aer and Tsr guide Escherichia coli in spatial gradients of oxidizable substrates Greer-Phillips, Suzanne E.; Alexandre, Gladys; Taylor, Barry L. Microbiology, Vol. 149, Issue 9 https://doi.org/10.1099/mic.0.26304-0	journal	September 2003
Predicting Protein Function by Genomic Context: Quantitative Evaluation and Qualitative Inferences Huynen, M. Genome Research, Vol. 10, Issue 8 https://doi.org/10.1101/gr.10.8.1204	journal	August 2000
Identification and characterization of the aspartate chemosensory receptor of Campylobacter jejuni: The aspartate receptor of Campylobacter jejuni Hartley-Tassell, Lauren E.; Shewell, Lucy K.; Day, Christopher J. Molecular Microbiology, Vol. 75, Issue 3 https://doi.org/10.1111/j.1365-2958.2009.07010.x	journal	December 2009
Salmonella chemoreceptors McpB and McpC mediate a repellent response to L-cystine: a potential mechanism to avoid oxidative conditions: McpB and McpC mediate a repellent response to L-cystine Lazova, Milena D.; Butler, Mitchell T.; Shimizu, Thomas S. Molecular Microbiology, Vol. 84, Issue 4 https://doi.org/10.1111/j.1365-2958.2012.08051.x	journal	April 2012
The Binding of Maltose to 'Virgin' Maltose-Binding Protein is Biphasic Hazelbauer, Gerald L. European Journal of Biochemistry, Vol. 60, Issue 2 https://doi.org/10.1111/j.1432-1033.1975.tb21022.x	journal	December 1975
Interactions among the three adaptation systems ofBacillus subtilischemotaxis as revealed by anin vitroreceptor-kinase assay Walukiewicz, Hanna E.; Tohidifar, Payman; Ordal, George W. Molecular Microbiology https://doi.org/10.1111/mmi.12721	journal	August 2014
Signalling-dependent interactions between the kinase-coupling protein CheW and chemoreceptors in living cells Pedetta, Andrea; Parkinson, John S.; Studdert, Claudia A. Molecular Microbiology https://doi.org/10.1111/mmi.12727	journal	August 2014
A Genomic Perspective on Protein Families Tatusov, R. L. Science, Vol. 278, Issue 5338 https://doi.org/10.1126/science.278.5338.631	journal	October 1997
Origins and Diversification of a Complex Signal Transduction System in Prokaryotes Wuichet, K.; Zhulin, I. B. Science Signaling, Vol. 3, Issue 128 https://doi.org/10.1126/scisignal.2000724	journal	June 2010
Uncovering a Large Set of Genes That Affect Surface Motility in Salmonella enterica Serovar Typhimurium Wang, Qingfeng; Mariconda, Susana; Suzuki, Asaka Journal of Bacteriology, Vol. 188, Issue 22 https://doi.org/10.1128/jb.00852-06	journal	September 2006
Negative Chemotaxis in Escherichia coli Tso, Wung-Wai; Adler, Julius Journal of Bacteriology, Vol. 118, Issue 2 https://doi.org/10.1128/jb.118.2.560-576.1974	journal	January 1974
Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium. DeFranco, A. L.; Parkinson, J. S.; Koshland, D. E. Journal of Bacteriology, Vol. 139, Issue 1 https://doi.org/10.1128/jb.139.1.107-114.1979	journal	January 1979
Identification of the tip-encoded receptor in bacterial sensing. Russo, A. F.; Koshland, D. E. Journal of Bacteriology, Vol. 165, Issue 1 https://doi.org/10.1128/jb.165.1.276-282.1986	journal	January 1986
A signal transducer for aerotaxis in Escherichia coli. Bibikov, S. I.; Biran, R.; Rudd, K. E. Journal of Bacteriology, Vol. 179, Issue 12 https://doi.org/10.1128/jb.179.12.4075-4079.1997	journal	June 1997
Different Evolutionary Constraints on Chemotaxis Proteins CheW and CheY Revealed by Heterologous Expression Studies and Protein Sequence Analysis Alexandre, Gladys; Zhulin, Igor B. Journal of Bacteriology, Vol. 185, Issue 2 https://doi.org/10.1128/jb.185.2.544-552.2003	journal	January 2003
Identification of New Flagellar Genes of Salmonella enterica Serovar Typhimurium Frye, Jonathan; Karlinsey, Joyce E.; Felise, Heather R. Journal of Bacteriology, Vol. 188, Issue 6 https://doi.org/10.1128/jb.188.6.2233-2243.2006	journal	March 2006
ggplot2 Wickham, Hadley Springer New York, NY https://doi.org/10.1007/978-0-387-98141-3	book	January 2009
Transposon-insertion mutants of Escherichia coli K12 defective in a component common to galactose and ribose chemotaxis Harayama, Shigeaki; Palva, E. Tapio; Hazelbauer, Gerald L. Molecular and General Genetics MGG, Vol. 171, Issue 2 https://doi.org/10.1007/BF00270005	journal	January 1979
Basic local alignment search tool Altschul, Stephen F.; Gish, Warren; Miller, Webb Journal of Molecular Biology, Vol. 215, Issue 3, p. 403-410 https://doi.org/10.1016/S0022-2836(05)80360-2	journal	October 1990
Structure of CheA, a Signal-Transducing Histidine Kinase Bilwes, Alexandrine M.; Alex, Lisa A.; Crane, Brian R. Cell, Vol. 96, Issue 1 https://doi.org/10.1016/S0092-8674(00)80966-6	journal	January 1999
A perspective on non-catalytic Src homology (SH) adaptor signalling proteins Reebye, Vikash; Frilling, Andrea; Hajitou, Amin Cellular Signalling, Vol. 24, Issue 2 https://doi.org/10.1016/j.cellsig.2011.10.003	journal	February 2012
Bacterial chemoreceptors: providing enhanced features to two-component signaling Hazelbauer, Gerald L.; Lai, Wing-Cheung Current Opinion in Microbiology, Vol. 13, Issue 2 https://doi.org/10.1016/j.mib.2009.12.014	journal	April 2010
Conserved Glycine Residues in the Cytoplasmic Domain of the Aspartate Receptor Play Essential Roles in Kinase Coupling and On−Off Switching ^† Coleman, Matthew D.; Bass, Randal B.; Mehan, Ryan S. Biochemistry, Vol. 44, Issue 21 https://doi.org/10.1021/bi0501479	journal	May 2005
Identification of a Site Critical for Kinase Regulation on the Central Processing Unit (CPU) Helix of the Aspartate Receptor ^† Trammell, Matthew A.; Falke, Joseph J. Biochemistry, Vol. 38, Issue 1 https://doi.org/10.1021/bi981964u	journal	January 1999
Peptide chemotaxis in E. coli involves the Tap signal transducer and the dipeptide permease Manson, Michael D.; Blank, Volker; Brade, Gabriele Nature, Vol. 321, Issue 6067 https://doi.org/10.1038/321253a0	journal	May 1986
Molecular model of a lattice of signalling proteins involved in bacterial chemotaxis Shimizu, Thomas S.; Le Novère, Nicolas; Levin, Matthew D. Nature Cell Biology, Vol. 2, Issue 11 https://doi.org/10.1038/35041030	journal	October 2000
A phenylalanine rotameric switch for signal-state control in bacterial chemoreceptors Ortega, Davi R.; Yang, Chen; Ames, Peter Nature Communications, Vol. 4, Issue 1 https://doi.org/10.1038/ncomms3881	journal	December 2013
Evolutionary genomics reveals conserved structural determinants of signaling and adaptation in microbial chemoreceptors Alexander, R. P.; Zhulin, I. B. Proceedings of the National Academy of Sciences, Vol. 104, Issue 8 https://doi.org/10.1073/pnas.0609359104	journal	February 2007
Collaborative signaling by mixed chemoreceptor teams in Escherichia coli Ames, P.; Studdert, C. A.; Reiser, R. H. Proceedings of the National Academy of Sciences, Vol. 99, Issue 10 https://doi.org/10.1073/pnas.092071899	journal	April 2002
The Aer protein and the serine chemoreceptor Tsr independently sense intracellular energy levels and transduce oxygen, redox, and energy signals for Escherichia coli behavior Rebbapragada, A.; Johnson, M. S.; Harding, G. P. Proceedings of the National Academy of Sciences, Vol. 94, Issue 20 https://doi.org/10.1073/pnas.94.20.10541	journal	September 1997
Phosphorylation of the Response Regulator CheV Is Required for Adaptation to Attractants during Bacillus subtilis Chemotaxis Karatan, Ece; Saulmon, Michael M.; Bunn, Michael W. Journal of Biological Chemistry, Vol. 276, Issue 47 https://doi.org/10.1074/jbc.M104955200	journal	September 2001
CheW Binding Interactions with CheA and Tar: IMPORTANCE FOR CHEMOTAXIS SIGNALING IN ESCHERICHIA COLI Boukhvalova, Marina S.; Dahlquist, Frederick W.; Stewart, Richard C. Journal of Biological Chemistry, Vol. 277, Issue 25 https://doi.org/10.1074/jbc.M110908200	journal	March 2002
Differential Recognition of Citrate and a Metal-Citrate Complex by the Bacterial Chemoreceptor Tcp Iwama, Tomonori; Ito, Yasuaki; Aoki, Hisaaki Journal of Biological Chemistry, Vol. 281, Issue 26 https://doi.org/10.1074/jbc.M601038200	journal	April 2006
Recent developments in the MAFFT multiple sequence alignment program Katoh, K.; Toh, H. Briefings in Bioinformatics, Vol. 9, Issue 4 https://doi.org/10.1093/bib/bbn013	journal	March 2008
Profile hidden Markov models Eddy, S. R. Bioinformatics, Vol. 14, Issue 9 https://doi.org/10.1093/bioinformatics/14.9.755	journal	October 1998
Protein robustness promotes evolutionary innovations on large evolutionary time-scales Ferrada, Evandro; Wagner, Andreas Proceedings of the Royal Society B: Biological Sciences, Vol. 275, Issue 1643 https://doi.org/10.1098/rspb.2007.1617	journal	April 2008
WebLogo: A Sequence Logo Generator Crooks, Gavin E.; Hon, Gary; Chandonia, John-Marc Genome Research, Vol. 14, Issue 6, p. 1188-1190 https://doi.org/10.1101/gr.849004	journal	May 2004
Python for Scientific Computing Oliphant, Travis E. Computing in Science & Engineering, Vol. 9, Issue 3 https://doi.org/10.1109/MCSE.2007.58	journal	January 2007
Protein Dynamism and Evolvability Tokuriki, N.; Tawfik, D. S. Science, Vol. 324, Issue 5924 https://doi.org/10.1126/science.1169375	journal	April 2009
Chemoreceptor Gene Loss and Acquisition via Horizontal Gene Transfer in Escherichia coli Borziak, K.; Fleetwood, A. D.; Zhulin, I. B. Journal of Bacteriology, Vol. 195, Issue 16 https://doi.org/10.1128/JB.00421-13	journal	June 2013
Uncovering a Large Set of Genes That Affect Surface Motility in Salmonella enterica Serovar Typhimurium Wang, Qingfeng; Mariconda, Susana; Suzuki, Asaka Journal of Bacteriology, Vol. 188, Issue 22 https://doi.org/10.1128/JB.00852-06	journal	September 2006
Chemotaxis to the Quorum-Sensing Signal AI-2 Requires the Tsr Chemoreceptor and the Periplasmic LsrB AI-2-Binding Protein Hegde, M.; Englert, D. L.; Schrock, S. Journal of Bacteriology, Vol. 193, Issue 3 https://doi.org/10.1128/JB.01196-10	journal	November 2010
Chemotaxis of Escherichia coli to Pyrimidines: a New Role for the Signal Transducer Tap Liu, Xianxian; Parales, Rebecca E. Journal of Bacteriology, Vol. 190, Issue 3 https://doi.org/10.1128/JB.01590-07	journal	December 2007
Negative Chemotaxis in Escherichia coli Tso, Wung-Wai; Adler, Julius Journal of Bacteriology, Vol. 118, Issue 2 https://doi.org/10.1128/JB.118.2.560-576.1974	journal	January 1974
Functional homology of chemotaxis genes in Escherichia coli and Salmonella typhimurium. DeFranco, A. L.; Parkinson, J. S.; Koshland, D. E. Journal of Bacteriology, Vol. 139, Issue 1 https://doi.org/10.1128/JB.139.1.107-114.1979	journal	January 1979
Different Evolutionary Constraints on Chemotaxis Proteins CheW and CheY Revealed by Heterologous Expression Studies and Protein Sequence Analysis Alexandre, Gladys; Zhulin, Igor B. Journal of Bacteriology, Vol. 185, Issue 2 https://doi.org/10.1128/JB.185.2.544-552.2003	journal	January 2003
Identification of New Flagellar Genes of Salmonella enterica Serovar Typhimurium Frye, Jonathan; Karlinsey, Joyce E.; Felise, Heather R. Journal of Bacteriology, Vol. 188, Issue 6 https://doi.org/10.1128/JB.188.6.2233-2243.2006	journal	March 2006
Accelerated Profile HMM Searches Eddy, Sean R. PLoS Computational Biology, Vol. 7, Issue 10 https://doi.org/10.1371/journal.pcbi.1002195	journal	October 2011
Conformational Coupling between Receptor and Kinase Binding Sites through a Conserved Salt Bridge in a Signaling Complex Scaffold Protein Ortega, Davi R.; Mo, Guoya; Lee, Kwangwoon PLoS Computational Biology, Vol. 9, Issue 11 https://doi.org/10.1371/journal.pcbi.1003337	journal	November 2013
Azospirillum Genomes Reveal Transition of Bacteria from Aquatic to Terrestrial Environments Wisniewski-Dyé, Florence; Borziak, Kirill; Khalsa-Moyers, Gurusahai PLoS Genetics, Vol. 7, Issue 12 https://doi.org/10.1371/journal.pgen.1002430	journal	December 2011
Structure of bacterial cytoplasmic chemoreceptor arrays and implications for chemotactic signaling Briegel, Ariane; Ladinsky, Mark S.; Oikonomou, Catherine eLife, Vol. 3 https://doi.org/10.7554/eLife.02151	journal	March 2014

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genomic databases
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Evolutionary genomics suggests that CheV is an additional adaptor for accommodating specific chemoreceptors within the chemotaxis signaling complex

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