Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance

Pang, Na; Bergeron, Andre David; Gu, Xiangyu; Fu, Xiao; Dong, Tao; Yao, Yiqing; Chen, Shulin

doi:10.1021/acs.est.0c02833

Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance

Journal Article · Sun Nov 15 00:00:00 EST 2020 · Environmental Science and Technology

DOI:https://doi.org/10.1021/acs.est.0c02833· OSTI ID:1755727

^[1]; Bergeron, Andre David ^[1]; Gu, Xiangyu ^[1]; Fu, Xiao ^[1]; ^[2]; Yao, Yiqing ^[3]; Chen, Shulin ^[1]

Washington State Univ., Pullman, WA (United States)
National Renewable Energy Lab. (NREL), Golden, CO (United States)
Northwest A & F Univ., Shaanxi (China)

This study explored the possibility of incorporating extremophilic algal cultivation into dairy wastewater treatment by characterizing a unique algal strain. Results showed that extremophilic microalgae Chlorella vulgaris CA1 newly isolated from dairy wastewater tolerated a high level of ammonia nitrogen (2.7 g/L), which was over 20 times the ammonia nitrogen that regular Chlorella sp. could tolerate. The isolate was mixotrophically cultured in dairy effluent treated by anaerobic digestion (AD) for recycling nutrients and polishing the wastewater. The highest biomass content of 13.3 g/L and protein content of 43.4% were achieved in the culture in AD effluent. Up to 96% of the total nitrogen and 79% of the total phosphorus were removed from the dairy AD effluent. The ability of the algae to tolerate a high level of ammonia nitrogen suggests the potential for direct nutrient recycling from dairy wastewater while producing algal biomass and high value bioproducts.

View Accepted Manuscript (DOE)

Research Organization:: National Renewable Energy Laboratory (NREL), Golden, CO (United States)

Sponsoring Organization:: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Bioenergy Technologies Office (EE-3B)

Grant/Contract Number:: AC36-08GO28308

OSTI ID:: 1755727

Report Number(s):: NREL/JA--2800-78276; MainId:32193; UUID:39a7df61-863d-4f5c-9880-dac8c48a4774; MainAdminID:19052

Journal Information:: Environmental Science and Technology, Journal Name: Environmental Science and Technology Journal Issue: 23 Vol. 54; ISSN 0013-936X

Publisher:: American Chemical Society (ACS)Copyright Statement

Country of Publication:: United States

Language:: English

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54 ENVIRONMENTAL SCIENCES
Chlorella vulgaris CA1
ammonia nitrogen tolerance
dairy wastewater treatment
mixotrophic culture
nutrient removal
protein-rich biomass

Recycling of Nutrients from Dairy Wastewater by Extremophilic Microalgae with High Ammonia Tolerance

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