Measuring the dynamic response of the thylakoid architecture in plant leaves by electron microscopy

Li, Meng; Mukhopadhyay, Roma; Svoboda, Václav; Oung, Hui Olivia; Mullendore, Daniel L.; Kirchhoff, Helmut

doi:10.1002/pld3.280

Measuring the dynamic response of the thylakoid architecture in plant leaves by electron microscopy

Journal Article · Thu Nov 05 00:00:00 EST 2020 · Plant Direct

DOI:https://doi.org/10.1002/pld3.280· OSTI ID:1735366

^[1]; Mukhopadhyay, Roma ^[2]; ^[2]; ^[2]; Mullendore, Daniel L. ^[3]; ^[2]

Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry; Washington State University
Washington State Univ., Pullman, WA (United States). Inst. of Biological Chemistry
Washington State Univ., Pullman, WA (United States). Franceschi Microscopy and Imaging Center

The performance of the photosynthesis machinery in plants, including light harvesting, electron transport, and protein repair is controlled by structural changes of the thylakoid membrane system inside the chloroplasts. In particular, the structure of the stacked grana area of thylakoid membranes is highly dynamic, changing in response to different environmental cues such as light intensity. For example, the aqueous thylakoid lumen enclosed by thylakoid membranes in grana has been documented to swell in the presence of light. However, light-induced alteration of the stromal gap in the stacked grana (partition gap) and of the unstacked stroma lamellae have not been well characterized. Light-induced changes of the entire thylakoid membrane system including the lumen in both stacked and unstacked domains as well as the partition gap are presented here, and the functional implications are discussed. This structural analysis was made possible by development of a robust semi-automated image analysis method combined with optimized plant tissue fixation techniques for transmission electron microscopy generating quantitative structural results for the analysis of thylakoid ultrastructure.

View Accepted Manuscript (DOE)

Research Organization:: Washington State Univ., Pullman, WA (United States)

Sponsoring Organization:: National Science Foundation (NSF); USDA; USDOE Office of Science (SC), Basic Energy Sciences (BES)

Grant/Contract Number:: SC0017160

OSTI ID:: 1735366

Alternate ID(s):: OSTI ID: 1735373

Journal Information:: Plant Direct, Journal Name: Plant Direct Journal Issue: 11 Vol. 4; ISSN 2475-4455

Publisher:: Wiley and American Society of Plant Biologists and Society for Experimental BiologyCopyright Statement

Country of Publication:: United States

Language:: English

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