Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

Li, Haoyuan; Nazari, Reza; Abbey, Brian; Alvarez, Roberto; Aquila, Andrew; Ayyer, Kartik; Barty, Anton; Berntsen, Peter; Bielecki, Johan; Pietrini, Alberto; Bucher, Maximilian; Carini, Gabriella; Chapman, Henry N.; Contreras, Alice; Daurer, Benedikt J.; DeMirci, Hasan; Flűckiger, Leonie; Frank, Matthias; Hajdu, Janos; Hantke, Max F.; Hogue, Brenda G.; Hosseinizadeh, Ahmad; Hunter, Mark S.; Jönsson, H. Olof; Kirian, Richard A.; Kurta, Ruslan P.; Loh, Duane; Maia, Filipe C.; Mancuso, Adrian P.; Morgan, Andrew J.; McFadden, Matthew; Muehlig, Kerstin; Munke, Anna; Reddy, Hemanth Narayana; Nettelblad, Carl; Ourmazd, Abbas; Rose, Max; Schwander, Peter; Marvin Seibert, M.; Sellberg, Jonas A.; Sierra, Raymond G.; Sun, Zhibin; Svenda, Martin; Vartanyants, Ivan A.; Walter, Peter; Westphal, Daniel; Williams, Garth; Xavier, P. Lourdu; Yoon, Chun Hong; Zaare, Sahba

doi:10.1038/s41597-020-00745-2

Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source

Journal Article · Wed Nov 18 23:00:00 EST 2020 · Scientific Data

DOI:https://doi.org/10.1038/s41597-020-00745-2· OSTI ID:1696907

^[1]; Nazari, Reza; ; ; Aquila, Andrew; ; Barty, Anton; ; Bielecki, Johan; Pietrini, Alberto; ; Carini, Gabriella; ; Contreras, Alice; Daurer, Benedikt J.; ; Flűckiger, Leonie; ; Hajdu, Janos; Hantke, Max F. more »

SLAC National Accelerator Laboratory (SLAC)

Single Particle Imaging (SPI) with intense coherent X-ray pulses from X-ray free-electron lasers (XFELs) has the potential to produce molecular structures without the need for crystallization or freezing. Here we present a dataset of 285,944 diffraction patterns from aerosolized Coliphage PR772 virus particles injected into the femtosecond X-ray pulses of the Linac Coherent Light Source (LCLS). Additional exposures with background information are also deposited. The diffraction data were collected at the Atomic, Molecular and Optical Science Instrument (AMO) of the LCLS in 4 experimental beam times during a period of four years. The photon energy was either 1.2 or 1.7 keV and the pulse energy was between 2 and 4 mJ in a focal spot of about 1.3 μm x 1.7 μm full width at half maximum (FWHM). The X-ray laser pulses captured the particles in random orientations. The data offer insight into aerosolised virus particles in the gas phase, contain information relevant to improving experimental parameters, and provide a basis for developing algorithms for image analysis and reconstruction.

View Accepted Manuscript (DOE)

Research Organization:: SLAC National Accelerator Laboratory, Menlo Park, CA (United States). Linac Coherent Light Source (LCLS)

Sponsoring Organization:: USDOE Office of Science (SC), Basic Energy Sciences (BES); National Science Foundation (NSF); Deutsche Forschungsgemeinschaft (DFG); National Institutes of Health (NIH); Helmholtz Association; Russian Science Foundation (RSF); European Union’s Horizon 2020; Swedish Research Council (VR); Knut and Alice Wallenberg Foundation; European Research Council (ERC); European Regional Development Fund (ERDF); Australian Research Council (ARC)

Contributing Organization:: SPI Team

Grant/Contract Number:: AC02-76SF00515; SC0002164

OSTI ID:: 1696907

Alternate ID(s):: OSTI ID: 1768618

Journal Information:: Scientific Data, Journal Name: Scientific Data Journal Issue: 1 Vol. 7; ISSN 2052-4463

Publisher:: Nature Publishing GroupCopyright Statement

Country of Publication:: United States

Language:: English

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Metadata record for: Diffraction data from aerosolized Coliphage PR772 virus particles imaged with the Linac Coherent Light Source Team, Scientific Data Curation figshare https://doi.org/10.6084/m9.figshare.13070546.v1	dataset	January 2020

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