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Coherent soft X-ray diffraction imaging of coliphage PR772 at the Linac coherent light source

Journal Article · · Scientific Data
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  1. Uppsala Univ., Uppsala (Sweden)
  2. SLAC National Accelerator Lab., Menlo Park, CA (United States)
  3. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany)
  4. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany)
  5. La Trobe Univ., Melbourne (Australia)
  6. National Research Centre 'Kurchatov Institute', Moscow (Russia)
  7. SLAC National Accelerator Lab., Menlo Park, CA (United States); Argonne National Lab. (ANL),Lemont, IL (United States); Technische Univ. Berlin, Berlin (Germany)
  8. SLAC National Accelerator Lab., Menlo Park, CA (United States); California Lutheran Univ., Thousand Oaks, CA (United States)
  9. Arizona State Univ., Tempe, AZ (United States)
  10. Uppsala Univ., Uppsala (Sweden); Institute of Physics AS CR, Prague 8 (Czech Republic)
  11. Univ. of Wisconsin, Milwaukee, WI (United States)
  12. Univ. of Science and Technology, Pohang (Korea)
  13. European X-ray Free Electron Laser GmbH, Schenefeld (Germany)
  14. National Univ. of Singapore (Singapore)
  15. Pohang Univ. of Science and Technology, Pohang (Korea)
  16. Uppsala Univ., Uppsala (Sweden); AlbaNova Univ. Center, Stockholm (Sweden)
  17. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); National Research Nuclear Univ. MEPhI (Moscow Engineering Physics Institute), Moscow (Russia)
  18. Brookhaven National Lab. (BNL), Upton, NY (United States)
  19. Deutsches Elektronen-Synchrotron (DESY), Hamburg (Germany); Univ. of Hamburg, Hamburg (Germany); Max-Planck Institute for the Structure and Dynamics of Matter, Hamburg (Germany)
+ Show Author Affiliations
Single-particle diffraction from X-ray Free Electron Lasers offers the potential for molecular structure determination without the need for crystallization. In an effort to further develop the technique, we present a dataset of coherent soft X-ray diffraction images of Coliphage PR772 virus, collected at the Atomic Molecular Optics (AMO) beamline with pnCCD detectors in the LAMP instrument at the Linac Coherent Light Source. The diameter of PR772 ranges from 65–70 nm, which is considerably smaller than the previously reported ~600 nm diameter Mimivirus. This reflects continued progress in XFEL-based single-particle imaging towards the single molecular imaging regime. As a result, the data set contains significantly more single particle hits than collected in previous experiments, enabling the development of improved statistical analysis, reconstruction algorithms, and quantitative metrics to determine resolution and self-consistency.
Research Organization:
SLAC National Accelerator Laboratory (SLAC), Menlo Park, CA (United States)
Sponsoring Organization:
USDOE
Grant/Contract Number:
AC02-76SF00515
OSTI ID:
1369312
Report Number(s):
SLAC-PUB-16952; sdata201779
Journal Information:
Scientific Data, Journal Name: Scientific Data Vol. 4; ISSN 2052-4463
Publisher:
Nature Publishing GroupCopyright Statement
Country of Publication:
United States
Language:
English

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dataset January 2016
Coherent Soft X-ray Diffraction Imaging of Coliphage PR772 at the Linac Coherent Light Source
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dataset January 2016
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Coherent Soft X-ray Diffraction Imaging of Coliphage PR772 at the Linac Coherent Light Source
  • Reddy, Hemanth
  • Coherent X-ray Imaging Data Bank (Lawrence Berkeley National Laboratory); Uppsala University, SLAC National Accelerator Laboratory, CFEL, University of Hamburg, Arizona State University, University of Wisconsin, La Trobe University https://doi.org/10.11577/1349664
dataset January 2017
Coherent diffraction of single Rice Dwarf virus particles using hard X-rays at the Linac Coherent Light Source text January 2016
Coherent diffraction of single Rice Dwarf Virus particles using hard X-rays at the Linac Coherent Light Source dataset July 2016

Cited By (28)

Fluctuation X-ray scattering journal December 2018
Parameter optimization for 3D-reconstruction from XFEL diffraction patterns based on Fourier slice matching journal January 2019
Conformational landscape of a virus by single-particle X-ray scattering journal August 2017
Megahertz single-particle imaging at the European XFEL journal May 2020
Ab initio structure determination from experimental fluctuation X-ray scattering data journal October 2018
Correlations in Scattered X-Ray Laser Pulses Reveal Nanoscale Structural Features of Viruses journal October 2017
Single-particle XFEL 3D reconstruction of ribosome-size particles based on Fourier slice matching: requirements to reach subnanometer resolution journal May 2018
Single-particle XFEL 3D reconstruction of ribosome-size particles based on Fourier slice matching: requirements to reach subnanometer resolution text January 2018
Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams journal September 2018
Rayleigh-scattering microscopy for tracking and sizing nanoparticles in focused aerosol beams text January 2018
Single-particle imaging without symmetry constraints at an X-ray free-electron laser journal September 2018
Biological single-particle imaging using XFELs – towards the next resolution revolution journal October 2018
Evaluation of the performance of classification algorithms for XFEL single-particle imaging data journal February 2019
Evaluation of the performance of classification algorithms for XFEL single-particle imaging data text January 2019
Experimental 3D coherent diffractive imaging from photon-sparse random projections journal March 2019
A guide to sample delivery systems for serial crystallography journal August 2019
Quantitative ptychographic bio-imaging in the water window journal January 2018
Supervised classification methods for flash X-ray single particle diffraction imaging journal January 2019
Low-signal limit of X-ray single particle diffractive imaging journal January 2019
Quantitative ptychographic bio-imaging in the water window text January 2018
Single-particle imaging without symmetry constraints at an X-ray free-electron laser text January 2018
Biological single-particle imaging using XFELs – towards the next resolution revolution text January 2018
Experimental 3D coherent diffractive imaging from photon-sparse random projections text January 2019
Evaluation of the performance of classification algorithms for XFEL single-particle imaging data text January 2019
Low-signal limit of X-ray single particle diffractive imaging text January 2019
Megahertz single-particle imaging at the European XFEL text January 2020
The Potential of EuPRAXIA@SPARC_LAB for Radiation Based Techniques journal March 2019
Electron cryo-microscopy of bacteriophage PR772 reveals the elusive vertex complex and the capsid architecture journal September 2019

Figures / Tables (5)

Figure 1(p. 2)figure Figure 1
Figure 2(p. 3)figure Figure 2
Figure 3(p. 4)figure Figure 3
Figure 4(p. 5)figure Figure 4
Table 1(p. 6)table Table 1

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