Converting 2D Nanofiber Membranes to 3D Hierarchical Assemblies with Structural and Compositional Gradients Regulates Cell Behavior

Chen, Shixuan; McCarthy, Alec; John, Johnson V.; Su, Yajuan; Xie, Jingwei

doi:10.1002/adma.202003754

Converting 2D Nanofiber Membranes to 3D Hierarchical Assemblies with Structural and Compositional Gradients Regulates Cell Behavior

Journal Article · Fri Sep 18 00:00:00 EDT 2020 · Advanced Materials

DOI:https://doi.org/10.1002/adma.202003754· OSTI ID:1686243

Chen, Shixuan ^[1]; McCarthy, Alec ^[1]; John, Johnson V. ^[1]; Su, Yajuan ^[1]; ^[2]

Department of Surgery‐Transplant and Mary &, Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha NE 68198 USA
Department of Surgery‐Transplant and Mary &, Dick Holland Regenerative Medicine Program University of Nebraska Medical Center Omaha NE 68198 USA, Department of Mechanical and Materials Engineering College of Engineering University of Nebraska‐Lincoln Lincoln NE 68588 USA

Abstract

New methods are described for converting 2D electrospun nanofiber membranes to 3D hierarchical assemblies with structural and compositional gradients. Pore‐size gradients are generated by tuning the expansion of 2D membranes in different layers with incorporation of various amounts of a surfactant during the gas‐foaming process. The gradient in fiber organizations is formed by expanding 2D nanofiber membranes composed of multiple regions collected by varying rotating speeds of mandrel. A compositional gradient on 3D assemblies consisting of radially aligned nanofibers is prepared by dripping, diffusion, and crosslinking. Bone mesenchymal stem cells (BMSCs) on the 3D nanofiber assemblies with smaller pore size show significantly higher expression of hypoxia‐related markers and enhanced chondrogenic differentiation compared to BMSCs cultured on the assemblies with larger pore size. The basic fibroblast growth factor gradient can accelerate fibroblast migration from the surrounding area to the center in an in vitro wound healing model. Taken together, 3D nanofiber assemblies with gradients in pore sizes, fiber organizations, and contents of signaling molecules can be used to engineer tissue constructs for tissue repair and build biomimetic disease models for studying disease biology and screening drugs, in particular, for interface tissue engineering and modeling.

View Accepted Manuscript (Publisher)

Sponsoring Organization:: USDOE

OSTI ID:: 1686243

Journal Information:: Advanced Materials, Journal Name: Advanced Materials Journal Issue: 43 Vol. 32; ISSN 0935-9648

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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