An automated platform for in situ serial crystallography at room temperature
Direct observation of functional motions in protein structures is highly desirable for understanding how these nanomachineries of life operate at the molecular level. Because cryogenic temperatures are non-physiological and may prohibit or even alter protein structural dynamics, it is necessary to develop robust X-ray diffraction methods that enable routine data collection at room temperature. We recently reported a crystal-on-crystal device to facilitate in situ diffraction of protein crystals at room temperature devoid of any sample manipulation. Here an automated serial crystallography platform based on this crystal-on-crystal technology is presented. A hardware and software prototype has been implemented, and protocols have been established that allow users to image, recognize and rank hundreds to thousands of protein crystals grown on a chip in optical scanning mode prior to serial introduction of these crystals to an X-ray beam in a programmable and high-throughput manner. This platform has been tested extensively using fragile protein crystals. We demonstrate that with affordable sample consumption, this in situ serial crystallography technology could give rise to room-temperature protein structures of higher resolution and superior map quality for those protein crystals that encounter difficulties during freezing. This serial data collection platform is compatible with both monochromatic oscillation and Laue methods for X-ray diffraction and presents a widely applicable approach for static and dynamic crystallographic studies at room temperature.
- Research Organization:
- Argonne National Lab. (ANL), Argonne, IL (United States). Advanced Photon Source (APS)
- Sponsoring Organization:
- USDOE Office of Science (SC), Basic Energy Sciences (BES). Scientific User Facilities Division; National Institutes of Health (NIH); National Science Foundation (NSF); Michigan Economic Development Corporation and Michigan Technology Tri-Corridor
- Grant/Contract Number:
- AC02-06CH11357; R01EY024363; MCB2017274; R24GM111072; 085P1000817
- OSTI ID:
- 1664540
- Alternate ID(s):
- OSTI ID: 1701779
- Journal Information:
- IUCrJ, Journal Name: IUCrJ Vol. 7 Journal Issue: 6; ISSN 2052-2525
- Publisher:
- International Union of Crystallography (IUCr)Copyright Statement
- Country of Publication:
- United Kingdom
- Language:
- English
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