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Insight into a highly polymorphic endophyte isolated from the roots of the halophytic seepweed Suaeda salsa: Laburnicola rhizohalophila sp. nov. (Didymosphaeriaceae, Pleosporales)

Journal Article · · Fungal Biology
 [1];  [2];  [3];  [3];  [1];  [4]
  1. Chinese Academy of Forestry, Beijing (China); Chinese Academy of Forestry, Hangzhou (China)
  2. Nanjing Agricultural Univ. (China)
  3. Chinese Academy of Forestry, Hangzhou (China)
  4. Oak Ridge National Lab. (ORNL), Oak Ridge, TN (United States)
+ Show Author Affiliations
In this work, we surveyed root endophytic fungi of the coastal halophyte Suaeda salsa and detected a population of a novel species that we described here as Laburnicola rhizohalophila sp. nov. No sexual sporulating structure was observed. Instead, it produced a large amount of thalloconidia, 0–1 transverse septa, hyaline to darkly pigmented, often peanut-shaped and sometimes dumbbell-shaped, both ends enlarged with numerous oil droplets inside the hyphal cells. Surprisingly, a high degree of phenotypic and physiological intraspecific variation (e.g., salinity tolerance, growth under different carbon:nitrogen ratios, and carbon utilization pattern) was recorded. The inoculation test indicated that the isolates could successfully infect host roots and form microsclerotia-like structures in cortical cells, a typical trait of dark septate endophytes (DSEs). Furthermore, most isolates were shown to promote host seedling growth. To evaluate conspecificity and infer its phylogenetic affinity, multiloci data including nuclear rRNA loci (ITS1 and 2, partial 28S), partial RNA Polymerase II second-largest subunit (rpb2), and partial translation elongation factor-1α (tef1) were characterized. Genealogical concordance phylogenetic species recognition (GCPSR) detected a genetically isolated clade of L. rhizohalophila within the Pleosporales in the Didymosphaeriaceae. Maximum likelihood phylogenetic reconstruction revealed that the endophytic fungus was genetically close to Laburnicola dactylidis but separated by a relatively long genetic distance. Our work highlights that the pleosporalean taxa might represent an underexplored reservoir of root DSEs.
Research Organization:
Oak Ridge National Laboratory (ORNL), Oak Ridge, TN (United States)
Sponsoring Organization:
Coordenaçao de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP); National Natural Science Foundation of China (NSFC); USDOE; USDOE Office of Science (SC), Biological and Environmental Research (BER) (SC-23)
Grant/Contract Number:
AC05-00OR22725
OSTI ID:
1648877
Alternate ID(s):
OSTI ID: 1780043
Journal Information:
Fungal Biology, Journal Name: Fungal Biology Journal Issue: 5 Vol. 124; ISSN 1878-6146
Publisher:
ElsevierCopyright Statement
Country of Publication:
United States
Language:
English

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Cited By (1)

Molecular phylogeny and diversity of Laburnicola (Didymosphaeriaceae): a new species from Uzbekistan journal December 2021

Figures / Tables (12)

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Related Subjects

59 BASIC BIOLOGICAL SCIENCES
Carbon utilization
Dark septate endophytes
Intraspecific polymorphism
Root-fungal mutualism