Elektroenzymatische Stickstofffixierung unter Verwendung eines MoFe‐Proteinsystems immobilisiert in einem organischen Redoxpolymer

Lee, Yoo Seok; Ruff, Adrian; Cai, Rong; Lim, Koun; Schuhmann, Wolfgang; Minteer, Shelley D.

doi:10.1002/ange.202007198

Elektroenzymatische Stickstofffixierung unter Verwendung eines MoFe‐Proteinsystems immobilisiert in einem organischen Redoxpolymer

Journal Article · Wed Jul 22 00:00:00 EDT 2020 · Angewandte Chemie

DOI:https://doi.org/10.1002/ange.202007198· OSTI ID:1641852

Lee, Yoo Seok ^[1]; ^[2]; Cai, Rong ^[3]; Lim, Koun ^[1]; ^[2]; Minteer, Shelley D. ^[1]

Department of Chemistry University of Utah 315 S 1400 E Salt Lake City Utah 84112 USA
Analytical Chemistry – Center for Electrochemical Sciences (CES) Faculty of Chemistry and Biochemistry Ruhr University Bochum Universitätsstr. 150 44780 Bochum Deutschland
Department of Chemistry University of Utah 315 S 1400 E Salt Lake City Utah 84112 USA, Department of Chemistry University of California Berkeley California 94720 USA

Abstract

Wir berichten über ein auf einem Polymer basierendes, elektroenzymatisches Stickstofffixierungssystem unter Verwendung eines metallfreien Redoxpolymers – mit Neutralrot modifiziertes Poly(glycidylmethacrylat‐ co ‐methylmethacrylat‐ co ‐poly(ethylenglycol)methacrylat), das ein niedriges Redoxpotential von −0.58 V vs. SCE besitzt. Die stabile und effiziente elektrische Kontaktierung der Nitrogenase innerhalb der Redoxpolymermatrix ermöglicht eine mediierte Bioelektrokatalyse von N ₃ ⁻ , NO ₂ ⁻ und N ₂ zu NH ₃ , die durch das MoFe‐Protein über die polymergebundenen Redoxeinheiten, die in der Polymermatrix verteilt sind, katalysiert wird. Elektrolyse produzierte 209±30 nmol NH ₃ nmol MoFe ⁻¹ h ⁻¹ durch N ₂ ‐Reduktion. Die biosynthetische N ₂ ‐Reduktion zu NH ₃ wurde durch ¹⁵ N ₂ ‐Markierungsexperimente und NMR‐Analysen bestätigt.

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Sponsoring Organization:: USDOE

Grant/Contract Number:: SC0017845

OSTI ID:: 1641852

Alternate ID(s):: OSTI ID: 1641855

Journal Information:: Angewandte Chemie, Journal Name: Angewandte Chemie Journal Issue: 38 Vol. 132; ISSN 0044-8249

Publisher:: Wiley Blackwell (John Wiley & Sons)Copyright Statement

Country of Publication:: Germany

Language:: English

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