Development of a Novel Bioprocess for Synthesizing and Purifying Adipic Acid

Technical Report · Thu Jun 18 04:00:00 EDT 2020

DOI:https://doi.org/10.2172/1633787· OSTI ID:1633787

Tanjore, Deepti ^[1]

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1. Lawrence Berkeley National Lab. (LBNL), Berkeley, CA (United States)

Through DOE’s Bioenergy Technologies Office and the Energy Efficiency and Renewable Energy Small Business Voucher (SBV) Pilot, ZymoChem, Inc received support to work with the ABPDU to accelerate the development of a novel adipic acid (AA) bioprocess by evaluating technologies to demonstrate the following: 1) Process development of AA via ZymoChem’s carbon-conserving (C²) pathway in 2L bioreactors, using industrial feedstocks, and as a salt with hexamethylenediamine (HMDA), i.e., the industrially desired form of AA as AA-HMDA is the precursor for nylon 6,6 synthesis. 2) Pilot scale aerobic fermentation at the 300 L scale (stage 1) followed by a high cell density (HCD) anaerobic resting cell biotransformation (RCB) at the 50 L scale (stage 2). 3) Demonstration of a viable recovery process at the 50 L scale for purifying the product (i.e., AA-HMDA salt) and deliver multiple kg-quantity batches of purified product for further testing.

• Research Organization: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)
• Sponsoring Organization: USDOE Office of Energy Efficiency and Renewable Energy (EERE), Transportation Office. Bioenergy Technologies Office
• Contributing Organization: ZymoChem Inc., Alameda, CA (United States)
• DOE Contract Number: AC02-05CH11231
• OSTI ID: 1633787
• Report Number(s): LBNL--2001338; AWD00001946
• Country of Publication: United States
• Language: English

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