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Metribuzin resistance via enhanced metabolism in a multiple herbicide resistant Lolium rigidum population

Journal Article · · Pest Management Science
DOI:https://doi.org/10.1002/ps.5929· OSTI ID:1633706
 [1];  [2];  [2];  [2];  [2]
  1. College of Plant Science &, Technology Huazhong Agricultural University Wuhan P. R. China, Australian Herbicide Resistance Initiative (AHRI), School of Agriculture and Environment The University of Western Australia Crawley WA Australia
  2. Australian Herbicide Resistance Initiative (AHRI), School of Agriculture and Environment The University of Western Australia Crawley WA Australia
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Abstract BACKGROUND

The photosystem II (PSII)‐inhibiting herbicides are important for Australian farmers to control Lolium rigidum Gaud. and other weed species in trazine tolerant (TT)‐canola fields. A L. rigidum population (R) collected from a TT‐canola field from Western Australia showed multiple resistance to PSII, acetyl‐coenzyme A carboxylase (ACCase) and acetolactate synthase (ALS) inhibitors. The mechanisms of multiple resistance in this R population were determined.

RESULTS

The R population showed a low‐level (about 3.0‐fold) resistance to the PSII‐inhibiting herbicides metribuzin and atrazine. Sequencing of the psbA gene revealed no differences between the R and susceptible (S) sequences. Furthermore, [ 14 C]‐metribuzin experiments found no significant difference in metribuzin foliar uptake and translocation between the R and S plants. However, [ 14 C]‐metribuzin metabolism in R plants was 2.3‐fold greater than in S plants. The cytochrome P450 monooxygenase inhibitor piperonyl butoxide (PBO) enhanced plant mortality response to metribuzin and atrazine in both R and S populations. In addition, multiple resistance to ALS and ACCase inhibitors are due to known resistance mutations in ALS and ACCase genes.

CONCLUSION

The results demonstrate that enhanced metribuzin metabolism likely involving cytochrome P450 monooxygenase contributes to metribuzin resistance in Lolium rigidum. This is the first report of metabolic resistance to the PSII‐inhibiting herbicide metribuzin in Australian Lolium rigidum . © 2020 Society of Chemical Industry

Sponsoring Organization:
USDOE
OSTI ID:
1633706
Journal Information:
Pest Management Science, Journal Name: Pest Management Science Journal Issue: 11 Vol. 76; ISSN 1526-498X
Publisher:
Wiley Blackwell (John Wiley & Sons)Copyright Statement
Country of Publication:
United Kingdom
Language:
English

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