Effect of cytochrome P450 arachidonate metabolites on ion transport in rabbit kidney loop of Henle

Journal Article · Thu Feb 14 23:00:00 EST 1991 · Science (Washington, D.C.); (United States)

DOI:https://doi.org/10.1126/science.1846705· OSTI ID:5820347

Escalante, B; McGiff, J C ^[1]; Erlij, D ^[2]; Falck, J R ^[3]

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1. New York Medical College, Valhalla (United States)
2. State Univ. of New York, Brooklyn (United States)
3. Univ. of Texas, Dallas (United States)

In the medullary segment of the thick ascending limb of the loop of Henle (mTALH), arachidonic acid (AA) is metabolized by a cytochrome P450-dependent monooxygenase to products that affect ion transport. The linkage between changes in ion transport and AA metabolism in isolated cells of the mTALH was examined. AA produced a concentration-dependent inhibition of {sup 86}Rb uptake-an effect that was prevented by selective blockade of cytochrome P450 monooxygenases. Inhibition by cytochrome 450 blockade of the effect of AA on {sup 86}Rb uptake could be circumvented by addition of the principal products of AA metabolism in the mTALH.

• OSTI ID: 5820347
• Journal Information: Science (Washington, D.C.); (United States), Journal Name: Science (Washington, D.C.); (United States) Vol. 251:4995; ISSN SCIEA; ISSN 0036-8075
• Country of Publication: United States
• Language: English

Related Subjects

550501* -- Metabolism-- Tracer Techniques
59 BASIC BIOLOGICAL SCIENCES
ANIMALS
ARACHIDONIC ACID
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CATIONS
CHARGED PARTICLES
DAYS LIVING RADIOISOTOPES
DOSE-RESPONSE RELATIONSHIPS
ELECTRON CAPTURE RADIOISOTOPES
ENZYME ACTIVITY
ENZYMES
INHIBITION
INTERMEDIATE MASS NUCLEI
IONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
KIDNEYS
MAMMALS
MEMBRANE TRANSPORT
METABOLISM
METABOLITES
MINUTES LIVING RADIOISOTOPES
MIXED-FUNCTION OXIDASES
MONOCARBOXYLIC ACIDS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
OXIDOREDUCTASES
OXYGENASES
PROTEINS
RABBITS
RADIOISOTOPES
RUBIDIUM 86
RUBIDIUM ISOTOP
TRACER TECHNIQUES
VERTEBRATES