At the nexus of three kingdoms: the genome of the mycorrhizal fungus Gigaspora margarita provides insights into plant, endobacterial and fungal interactions

Venice, Francesco; Ghignone, Stefano; Salvioli di Fossalunga, Alessandra; Amselem, Joëlle; Novero, Mara; Xianan, Xie; Sędzielewska Toro, Kinga; Morin, Emmanuelle; Lipzen, Anna; Grigoriev, Igor V.; Henrissat, Bernard; Martin, Francis M.; Bonfante, Paola

doi:10.1111/1462-2920.14827

At the nexus of three kingdoms: the genome of the mycorrhizal fungus Gigaspora margarita provides insights into plant, endobacterial and fungal interactions

Journal Article · Wed Oct 16 00:00:00 EDT 2019 · Environmental Microbiology

DOI:https://doi.org/10.1111/1462-2920.14827· OSTI ID:1630616

Venice, Francesco ^[1]; ^[2]; Salvioli di Fossalunga, Alessandra ^[1]; Amselem, Joëlle ^[3]; Novero, Mara ^[1]; Xianan, Xie ^[4]; Sędzielewska Toro, Kinga ^[5]; Morin, Emmanuelle ^[6]; Lipzen, Anna ^[7]; Grigoriev, Igor V. ^[8]; Henrissat, Bernard ^[9]; Martin, Francis M. ^[6]; ^[1]

Univ. of Turin (Italy)
Inst. for Sustainable Plant Protection, Turin (Italy)
Univ. Paris‐Saclay, Versailles (France)
South China Agricultural Univ., Guangzhou (China)
Ludwig‐Maximilians‐Univ. Munich, Planegg‐Martinsried (Germany)
Inst. National de la Recherche Agronomique (INRA), Champenoux (France)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States); Univ. of California, Berkeley, CA (United States)
USDOE Joint Genome Institute (JGI), Walnut Creek, CA (United States)
Aix‐Marseille Univ., Marseille (France); Institut National de la Recherche Agronomique (INRA), Marseille (France); King Abdulaziz Univ., Jeddah (Saudi Arabia)

As members of the plant microbiota, arbuscular mycorrhizal fungi (AMF, Glomeromycotina) symbiotically colonize plant roots. AMF also possess their own microbiota, hosting some uncultivable endobacteria. Ongoing research has revealed the genetics underlying plant responses to colonization by AMF, but the fungal side of the relationship remains in the dark. Here, we sequenced the genome of Gigaspora margarita, a member of the Gigasporaceae in an early diverging group of the Glomeromycotina. In contrast to other AMF, G. margarita may host distinct endobacterial populations and possesses the largest fungal genome so far annotated (773.104 Mbp), with more than 64% transposable elements. Other unique traits of the G. margarita genome include the expansion of genes for inorganic phosphate metabolism, the presence of genes for production of secondary metabolites and a considerable number of potential horizontal gene transfer events. Finally, the sequencing of G. margarita genome reveals the importance of its immune system, shedding light on the evolutionary pathways that allowed early diverging fungi to interact with both plants and bacteria.

View Accepted Manuscript (DOE)

Research Organization:: Lawrence Berkeley National Laboratory (LBNL), Berkeley, CA (United States)

Sponsoring Organization:: USDOE Office of Science (SC)

Grant/Contract Number:: AC02-05CH11231

OSTI ID:: 1630616

Journal Information:: Environmental Microbiology, Journal Name: Environmental Microbiology Journal Issue: 1 Vol. 22; ISSN 1462-2912

Publisher:: WileyCopyright Statement

Country of Publication:: United States

Language:: English

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Additional file 1 of Evolutionary history of arbuscular mycorrhizal fungi and genomic signatures of obligate symbiosis Rosling, Anna; Eshghi Sahraei, Shadi; Kalsoom Khan, Faheema figshare https://doi.org/10.6084/m9.figshare.25929209	dataset	January 2024